Microplastic particles are phagocytosed in gill cells of deep-sea and coastal mussels
It is becoming obvious that the abundance of microplastics is increasing in worldwide oceans, raising concerns about their impact on marine ecosystems. Tiny plastic particles enter the body of marine organisms not only via oral ingestion but also through the body surface (e.g., gills or epidermis),...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Marine Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2022.1034950/full |
| _version_ | 1811235071172018176 |
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| author | Tetsuro Ikuta Akihiro Tame Tomoko Takahashi Hidetaka Nomaki Ryota Nakajima |
| author_facet | Tetsuro Ikuta Akihiro Tame Tomoko Takahashi Hidetaka Nomaki Ryota Nakajima |
| author_sort | Tetsuro Ikuta |
| collection | DOAJ |
| description | It is becoming obvious that the abundance of microplastics is increasing in worldwide oceans, raising concerns about their impact on marine ecosystems. Tiny plastic particles enter the body of marine organisms not only via oral ingestion but also through the body surface (e.g., gills or epidermis), but the mechanism of internalization into cells is poorly understood. In this study, we conducted experiments using deep-sea chemosynthetic mussels with limited feeding by exposing their gills to fluorescently labeled microplastic beads. We identified the gill cell types that preferentially internalized the beads and demonstrated the inhibitory effect of phagocytosis inhibitors on bead uptake. Furthermore, using correlative light-electron microscopy, we microhistologically verified that beads were enclosed within membrane-bound vacuoles. Our results indicated that microplastic particles were internalized into gill cells of deep-sea and coastal mussels by phagocytosis. This study highlights the need for further research on plastic contamination via the body surface to conserve the highly endemic and vulnerable deep-sea fauna and mitigate human health risks from consuming coastal bivalves. |
| first_indexed | 2024-04-12T11:45:59Z |
| format | Article |
| id | doaj.art-3adef6a7d2594c81a5cfc516341e2669 |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-04-12T11:45:59Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj.art-3adef6a7d2594c81a5cfc516341e26692022-12-22T03:34:22ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452022-10-01910.3389/fmars.2022.10349501034950Microplastic particles are phagocytosed in gill cells of deep-sea and coastal musselsTetsuro Ikuta0Akihiro Tame1Tomoko Takahashi2Hidetaka Nomaki3Ryota Nakajima4Marine Biodiversity and Environmental Assessment Research Center (BioEnv), Research Institute for Global Change (RIGC), Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokosuka, JapanDepartment of Marine and Earth Sciences, Marine Works Japan Ltd., Yokosuka, JapanMarine Biodiversity and Environmental Assessment Research Center (BioEnv), Research Institute for Global Change (RIGC), Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokosuka, JapanInstitute for Extra-cutting-edge Science and Technology Avant-garde Research (X-star), Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokosuka, JapanMarine Biodiversity and Environmental Assessment Research Center (BioEnv), Research Institute for Global Change (RIGC), Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokosuka, JapanIt is becoming obvious that the abundance of microplastics is increasing in worldwide oceans, raising concerns about their impact on marine ecosystems. Tiny plastic particles enter the body of marine organisms not only via oral ingestion but also through the body surface (e.g., gills or epidermis), but the mechanism of internalization into cells is poorly understood. In this study, we conducted experiments using deep-sea chemosynthetic mussels with limited feeding by exposing their gills to fluorescently labeled microplastic beads. We identified the gill cell types that preferentially internalized the beads and demonstrated the inhibitory effect of phagocytosis inhibitors on bead uptake. Furthermore, using correlative light-electron microscopy, we microhistologically verified that beads were enclosed within membrane-bound vacuoles. Our results indicated that microplastic particles were internalized into gill cells of deep-sea and coastal mussels by phagocytosis. This study highlights the need for further research on plastic contamination via the body surface to conserve the highly endemic and vulnerable deep-sea fauna and mitigate human health risks from consuming coastal bivalves.https://www.frontiersin.org/articles/10.3389/fmars.2022.1034950/fullmicroplasticphagocytosisgill epithelial celldeep-sea musselcoastal musselcorrelative light-electron microscopy |
| spellingShingle | Tetsuro Ikuta Akihiro Tame Tomoko Takahashi Hidetaka Nomaki Ryota Nakajima Microplastic particles are phagocytosed in gill cells of deep-sea and coastal mussels Frontiers in Marine Science microplastic phagocytosis gill epithelial cell deep-sea mussel coastal mussel correlative light-electron microscopy |
| title | Microplastic particles are phagocytosed in gill cells of deep-sea and coastal mussels |
| title_full | Microplastic particles are phagocytosed in gill cells of deep-sea and coastal mussels |
| title_fullStr | Microplastic particles are phagocytosed in gill cells of deep-sea and coastal mussels |
| title_full_unstemmed | Microplastic particles are phagocytosed in gill cells of deep-sea and coastal mussels |
| title_short | Microplastic particles are phagocytosed in gill cells of deep-sea and coastal mussels |
| title_sort | microplastic particles are phagocytosed in gill cells of deep sea and coastal mussels |
| topic | microplastic phagocytosis gill epithelial cell deep-sea mussel coastal mussel correlative light-electron microscopy |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2022.1034950/full |
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