Functional analysis of hemolin gene from silkworm, Bombyx mori - immune and development
Hemolin, an immunoglobulin-like protein, is involved in the immunity and development of Lepi-doptera, but its exact functions are still unclear. In this work, the quantitative PCR and Western blot analysis were used to study the effects of exogenous substances on hemolin transcription and transla-ti...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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University of Modena and Reggio Emilia
2017-08-01
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| Series: | Invertebrate Survival Journal |
| Subjects: | |
| Online Access: | https://isj02.unimore.it/index.php/ISJ/article/view/58 |
| _version_ | 1819279709355114496 |
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| author | X Liu J Li Q Wang H Xia K Chen |
| author_facet | X Liu J Li Q Wang H Xia K Chen |
| author_sort | X Liu |
| collection | DOAJ |
| description | Hemolin, an immunoglobulin-like protein, is involved in the immunity and development of Lepi-doptera, but its exact functions are still unclear. In this work, the quantitative PCR and Western blot analysis were used to study the effects of exogenous substances on hemolin transcription and transla-tion in the silkworm midgut. Also, RNA interference was used to examine the effects of hemolin on wing disc development of silkworm. we found that the transcription and expression of hemolin gene could be up-regulated effectively after injection of inactive Escherichia coli and cell wall components (LPS and PGN) into silkworm midgut. However, no any changes in transcription and expression of hemolin were observed by the injection of BmNPV (Bombyx mori nuclear polyhydrosis virus). The protein-protein interaction between hemolin and yippee was verified by Co-immunoprecipitation (Co-IP) in silkworm wing discs. In addition, the silence of hemolin gene by RNA interference could lead to adult wing hy-poplasia in most hatched moths. Our study provides some useful information regarding the functions of hemolin associated with the immune and developmental mechanisms in silkworm. |
| first_indexed | 2024-12-24T00:32:13Z |
| format | Article |
| id | doaj.art-3ae0ef7a8d6845fd8f3bf78a58a92285 |
| institution | Directory Open Access Journal |
| issn | 1824-307X |
| language | English |
| last_indexed | 2024-12-24T00:32:13Z |
| publishDate | 2017-08-01 |
| publisher | University of Modena and Reggio Emilia |
| record_format | Article |
| series | Invertebrate Survival Journal |
| spelling | doaj.art-3ae0ef7a8d6845fd8f3bf78a58a922852022-12-21T17:24:12ZengUniversity of Modena and Reggio EmiliaInvertebrate Survival Journal1824-307X2017-08-01141Functional analysis of hemolin gene from silkworm, Bombyx mori - immune and developmentX Liu0J Li1Q Wang2H Xia3K Chen4Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, P.R. ChinaInstitute of Life Sciences, Jiangsu University, Zhenjiang 212013, P.R. ChinaInstitute of Life Sciences, Jiangsu University, Zhenjiang 212013, P.R. ChinaInstitute of Life Sciences, Jiangsu University, Zhenjiang 212013, P.R. ChinaInstitute of Life Sciences, Jiangsu University, Zhenjiang 212013, P.R. ChinaHemolin, an immunoglobulin-like protein, is involved in the immunity and development of Lepi-doptera, but its exact functions are still unclear. In this work, the quantitative PCR and Western blot analysis were used to study the effects of exogenous substances on hemolin transcription and transla-tion in the silkworm midgut. Also, RNA interference was used to examine the effects of hemolin on wing disc development of silkworm. we found that the transcription and expression of hemolin gene could be up-regulated effectively after injection of inactive Escherichia coli and cell wall components (LPS and PGN) into silkworm midgut. However, no any changes in transcription and expression of hemolin were observed by the injection of BmNPV (Bombyx mori nuclear polyhydrosis virus). The protein-protein interaction between hemolin and yippee was verified by Co-immunoprecipitation (Co-IP) in silkworm wing discs. In addition, the silence of hemolin gene by RNA interference could lead to adult wing hy-poplasia in most hatched moths. Our study provides some useful information regarding the functions of hemolin associated with the immune and developmental mechanisms in silkworm.https://isj02.unimore.it/index.php/ISJ/article/view/58Bombyx moriimmunitydevelopmenthemolinyippee |
| spellingShingle | X Liu J Li Q Wang H Xia K Chen Functional analysis of hemolin gene from silkworm, Bombyx mori - immune and development Invertebrate Survival Journal Bombyx mori immunity development hemolin yippee |
| title | Functional analysis of hemolin gene from silkworm, Bombyx mori - immune and development |
| title_full | Functional analysis of hemolin gene from silkworm, Bombyx mori - immune and development |
| title_fullStr | Functional analysis of hemolin gene from silkworm, Bombyx mori - immune and development |
| title_full_unstemmed | Functional analysis of hemolin gene from silkworm, Bombyx mori - immune and development |
| title_short | Functional analysis of hemolin gene from silkworm, Bombyx mori - immune and development |
| title_sort | functional analysis of hemolin gene from silkworm bombyx mori immune and development |
| topic | Bombyx mori immunity development hemolin yippee |
| url | https://isj02.unimore.it/index.php/ISJ/article/view/58 |
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