Convergent evolution of argonaute-2 slicer antagonism in two distinct insect RNA viruses.
RNA interference (RNAi) is a major antiviral pathway that shapes evolution of RNA viruses. We show here that Nora virus, a natural Drosophila pathogen, is both a target and suppressor of RNAi. We detected viral small RNAs with a signature of Dicer-2 dependent small interfering RNAs in Nora virus inf...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS Pathogens |
| Online Access: | http://europepmc.org/articles/PMC3420963?pdf=render |
| _version_ | 1818900035136389120 |
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| author | Joël T van Mierlo Alfred W Bronkhorst Gijs J Overheul Sajna A Sadanandan Jens-Ola Ekström Marco Heestermans Dan Hultmark Christophe Antoniewski Ronald P van Rij |
| author_facet | Joël T van Mierlo Alfred W Bronkhorst Gijs J Overheul Sajna A Sadanandan Jens-Ola Ekström Marco Heestermans Dan Hultmark Christophe Antoniewski Ronald P van Rij |
| author_sort | Joël T van Mierlo |
| collection | DOAJ |
| description | RNA interference (RNAi) is a major antiviral pathway that shapes evolution of RNA viruses. We show here that Nora virus, a natural Drosophila pathogen, is both a target and suppressor of RNAi. We detected viral small RNAs with a signature of Dicer-2 dependent small interfering RNAs in Nora virus infected Drosophila. Furthermore, we demonstrate that the Nora virus VP1 protein contains RNAi suppressive activity in vitro and in vivo that enhances pathogenicity of recombinant Sindbis virus in an RNAi dependent manner. Nora virus VP1 and the viral suppressor of RNAi of Cricket paralysis virus (1A) antagonized Argonaute-2 (AGO2) Slicer activity of RNA induced silencing complexes pre-loaded with a methylated single-stranded guide strand. The convergent evolution of AGO2 suppression in two unrelated insect RNA viruses highlights the importance of AGO2 in antiviral defense. |
| first_indexed | 2024-12-19T19:57:27Z |
| format | Article |
| id | doaj.art-3ae122e55e5e4e25b6ea0cc96229f328 |
| institution | Directory Open Access Journal |
| issn | 1553-7366 1553-7374 |
| language | English |
| last_indexed | 2024-12-19T19:57:27Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj.art-3ae122e55e5e4e25b6ea0cc96229f3282022-12-21T20:07:45ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742012-01-0188e100287210.1371/journal.ppat.1002872Convergent evolution of argonaute-2 slicer antagonism in two distinct insect RNA viruses.Joël T van MierloAlfred W BronkhorstGijs J OverheulSajna A SadanandanJens-Ola EkströmMarco HeestermansDan HultmarkChristophe AntoniewskiRonald P van RijRNA interference (RNAi) is a major antiviral pathway that shapes evolution of RNA viruses. We show here that Nora virus, a natural Drosophila pathogen, is both a target and suppressor of RNAi. We detected viral small RNAs with a signature of Dicer-2 dependent small interfering RNAs in Nora virus infected Drosophila. Furthermore, we demonstrate that the Nora virus VP1 protein contains RNAi suppressive activity in vitro and in vivo that enhances pathogenicity of recombinant Sindbis virus in an RNAi dependent manner. Nora virus VP1 and the viral suppressor of RNAi of Cricket paralysis virus (1A) antagonized Argonaute-2 (AGO2) Slicer activity of RNA induced silencing complexes pre-loaded with a methylated single-stranded guide strand. The convergent evolution of AGO2 suppression in two unrelated insect RNA viruses highlights the importance of AGO2 in antiviral defense.http://europepmc.org/articles/PMC3420963?pdf=render |
| spellingShingle | Joël T van Mierlo Alfred W Bronkhorst Gijs J Overheul Sajna A Sadanandan Jens-Ola Ekström Marco Heestermans Dan Hultmark Christophe Antoniewski Ronald P van Rij Convergent evolution of argonaute-2 slicer antagonism in two distinct insect RNA viruses. PLoS Pathogens |
| title | Convergent evolution of argonaute-2 slicer antagonism in two distinct insect RNA viruses. |
| title_full | Convergent evolution of argonaute-2 slicer antagonism in two distinct insect RNA viruses. |
| title_fullStr | Convergent evolution of argonaute-2 slicer antagonism in two distinct insect RNA viruses. |
| title_full_unstemmed | Convergent evolution of argonaute-2 slicer antagonism in two distinct insect RNA viruses. |
| title_short | Convergent evolution of argonaute-2 slicer antagonism in two distinct insect RNA viruses. |
| title_sort | convergent evolution of argonaute 2 slicer antagonism in two distinct insect rna viruses |
| url | http://europepmc.org/articles/PMC3420963?pdf=render |
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