HPLC-DAD assay of flavonoids and evaluation of antioxidant activity of some herbal mixtures
Medicinal plants and their combinations can influence various links of the pathogenetic mechanism of diabetes mellitus type 2 and its complications, due to the wide range of biologically active substance that they accumulate. Flavonoids deserve particular attention through their antioxidant properti...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Pensoft Publishers
2022-09-01
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| Series: | Pharmacia |
| Online Access: | https://pharmacia.pensoft.net/article/86486/download/pdf/ |
| _version_ | 1798031798078275584 |
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| author | Alona Savych Svitlana Marchyshyn Olha Polonets Olga Mala Iryna Shcherba Liubov Morozova |
| author_facet | Alona Savych Svitlana Marchyshyn Olha Polonets Olga Mala Iryna Shcherba Liubov Morozova |
| author_sort | Alona Savych |
| collection | DOAJ |
| description | Medicinal plants and their combinations can influence various links of the pathogenetic mechanism of diabetes mellitus type 2 and its complications, due to the wide range of biologically active substance that they accumulate. Flavonoids deserve particular attention through their antioxidant properties. Three samples of herbal mixtures (sample 1 – Inula helenium rhizome with roots, Helichrysum arenarium flowers, Zea mays columns with stigmas, Origanum vulgare herb, Rosa majalis fruits, Taraxacum officinale roots; sample 2 – Cichorium intybus roots, Elymus repens rhizome, Helichrysum arenarium flowers, Rosa smajalis fruits, Zea mays columns with stigmas; sample 3 – Urtica dioica leaf, Taraxacum officinale roots, Vaccinium myrtillus leaf, Rosa majalis fruits, Mentha x Menthapiperita herb) were tested for flavonoid content and antioxidant properties. Using HPLC-DAD analysis the content of flavonoids was evaluated and an antioxidant activity by DPPH-radicals scavenging, ferrous ion chelating capacity and ferric reducing power were established for the herbal mixtures. Rutin prevails in sample 3, its content was 2745.66±0.21 μg/g; luteolin – in samples 1 and 2, its content was 371.31±0.07 μg/g and 313.48±0.13 μg/g, respectively. Flavonoids attribute to the antioxidant activity of the herbal mixtures, which was confirmed by DPPH radical scavenging assay, ferric reducing power assay and ferrous ion chelating assay. The highest antioxidant capacity was found for sample 3 – IC50 of inhibition of DPPH radicals was 301.65±2.67 µg/mL compared to control – ascorbic acid (119.24±2.35 µg/mL), the ferric reducing power was 0.382 at 100 µg/mL compared to ascorbic acid (0.412 at 100 µg/mL) and IC50 of chelating capacity was 206.59±2.48 µg/mL compared to EDTA-Na2 (110.55±1.93 µg/mL). |
| first_indexed | 2024-04-11T20:02:16Z |
| format | Article |
| id | doaj.art-3ae133e0d705488a834acdff51264b43 |
| institution | Directory Open Access Journal |
| issn | 2603-557X |
| language | English |
| last_indexed | 2024-04-11T20:02:16Z |
| publishDate | 2022-09-01 |
| publisher | Pensoft Publishers |
| record_format | Article |
| series | Pharmacia |
| spelling | doaj.art-3ae133e0d705488a834acdff51264b432022-12-22T04:05:30ZengPensoft PublishersPharmacia2603-557X2022-09-0169387388110.3897/pharmacia.69.e8648686486HPLC-DAD assay of flavonoids and evaluation of antioxidant activity of some herbal mixturesAlona Savych0Svitlana Marchyshyn1Olha Polonets2Olga Mala3Iryna Shcherba4Liubov Morozova5I. Horbachevsky Ternopil National Medical UniversityI. Horbachevsky Ternopil National Medical UniversityNational Pirogov Memorial Medical UniversityNational University of PharmacyNational Pirogov Memorial Medical UniversityVinnytsia National Agrarian UniversityMedicinal plants and their combinations can influence various links of the pathogenetic mechanism of diabetes mellitus type 2 and its complications, due to the wide range of biologically active substance that they accumulate. Flavonoids deserve particular attention through their antioxidant properties. Three samples of herbal mixtures (sample 1 – Inula helenium rhizome with roots, Helichrysum arenarium flowers, Zea mays columns with stigmas, Origanum vulgare herb, Rosa majalis fruits, Taraxacum officinale roots; sample 2 – Cichorium intybus roots, Elymus repens rhizome, Helichrysum arenarium flowers, Rosa smajalis fruits, Zea mays columns with stigmas; sample 3 – Urtica dioica leaf, Taraxacum officinale roots, Vaccinium myrtillus leaf, Rosa majalis fruits, Mentha x Menthapiperita herb) were tested for flavonoid content and antioxidant properties. Using HPLC-DAD analysis the content of flavonoids was evaluated and an antioxidant activity by DPPH-radicals scavenging, ferrous ion chelating capacity and ferric reducing power were established for the herbal mixtures. Rutin prevails in sample 3, its content was 2745.66±0.21 μg/g; luteolin – in samples 1 and 2, its content was 371.31±0.07 μg/g and 313.48±0.13 μg/g, respectively. Flavonoids attribute to the antioxidant activity of the herbal mixtures, which was confirmed by DPPH radical scavenging assay, ferric reducing power assay and ferrous ion chelating assay. The highest antioxidant capacity was found for sample 3 – IC50 of inhibition of DPPH radicals was 301.65±2.67 µg/mL compared to control – ascorbic acid (119.24±2.35 µg/mL), the ferric reducing power was 0.382 at 100 µg/mL compared to ascorbic acid (0.412 at 100 µg/mL) and IC50 of chelating capacity was 206.59±2.48 µg/mL compared to EDTA-Na2 (110.55±1.93 µg/mL).https://pharmacia.pensoft.net/article/86486/download/pdf/ |
| spellingShingle | Alona Savych Svitlana Marchyshyn Olha Polonets Olga Mala Iryna Shcherba Liubov Morozova HPLC-DAD assay of flavonoids and evaluation of antioxidant activity of some herbal mixtures Pharmacia |
| title | HPLC-DAD assay of flavonoids and evaluation of antioxidant activity of some herbal mixtures |
| title_full | HPLC-DAD assay of flavonoids and evaluation of antioxidant activity of some herbal mixtures |
| title_fullStr | HPLC-DAD assay of flavonoids and evaluation of antioxidant activity of some herbal mixtures |
| title_full_unstemmed | HPLC-DAD assay of flavonoids and evaluation of antioxidant activity of some herbal mixtures |
| title_short | HPLC-DAD assay of flavonoids and evaluation of antioxidant activity of some herbal mixtures |
| title_sort | hplc dad assay of flavonoids and evaluation of antioxidant activity of some herbal mixtures |
| url | https://pharmacia.pensoft.net/article/86486/download/pdf/ |
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