Silk–Inorganic Nanoparticle Hybrid Hydrogel as an Injectable Bone Repairing Biomaterial
Silk fibroin is regarded as a promising biomaterial in various areas, including bone tissue regeneration. Herein, Laponite<sup>®</sup> (LAP), which can promote osteogenic differentiation, was introduced into regenerated silk fibroin (RSF) to prepare an RSF/LAP hybrid hydrogel. This thixo...
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| Format: | Article |
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MDPI AG
2023-02-01
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| Series: | Journal of Functional Biomaterials |
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| Online Access: | https://www.mdpi.com/2079-4983/14/2/86 |
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| author | Liangyan Sun Minqi Lu Ling Chen Bingjiao Zhao Jinrong Yao Zhengzhong Shao Xin Chen Yuehua Liu |
| author_facet | Liangyan Sun Minqi Lu Ling Chen Bingjiao Zhao Jinrong Yao Zhengzhong Shao Xin Chen Yuehua Liu |
| author_sort | Liangyan Sun |
| collection | DOAJ |
| description | Silk fibroin is regarded as a promising biomaterial in various areas, including bone tissue regeneration. Herein, Laponite<sup>®</sup> (LAP), which can promote osteogenic differentiation, was introduced into regenerated silk fibroin (RSF) to prepare an RSF/LAP hybrid hydrogel. This thixotropic hydrogel is injectable during the operation process, which is favorable for repairing bone defects. Our previous work demonstrated that the RSF/LAP hydrogel greatly promoted the osteogenic differentiation of osteoblasts in vitro. In the present study, the RSF/LAP hydrogel was found to have excellent biocompatibility and significantly improved new bone formation in a standard rat calvarial defect model in vivo. Additionally, the underlying biological mechanism of the RSF/LAP hydrogel in promoting osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) was extensively explored. The results indicate that the RSF/LAP hydrogels provide suitable conditions for the adhesion and proliferation of BMSCs, showing good biocompatibility in vitro. With the increase in LAP content, the alkaline phosphatase (ALP) activity and mRNA and protein expression of the osteogenic markers of BMSCs improved significantly. Protein kinase B (AKT) pathway activation was found to be responsible for the inherent osteogenic properties of the RSF/LAP hybrid hydrogel. Therefore, the results shown in this study firmly suggest such an injectable RSF/LAP hydrogel with good biocompatibility (both in vitro and in vivo) would have good application prospects in the field of bone regeneration. |
| first_indexed | 2024-03-11T08:37:02Z |
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| institution | Directory Open Access Journal |
| issn | 2079-4983 |
| language | English |
| last_indexed | 2024-03-11T08:37:02Z |
| publishDate | 2023-02-01 |
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| series | Journal of Functional Biomaterials |
| spelling | doaj.art-3ae666c107404f7f97575d73e89bf3b72023-11-16T21:24:28ZengMDPI AGJournal of Functional Biomaterials2079-49832023-02-011428610.3390/jfb14020086Silk–Inorganic Nanoparticle Hybrid Hydrogel as an Injectable Bone Repairing BiomaterialLiangyan Sun0Minqi Lu1Ling Chen2Bingjiao Zhao3Jinrong Yao4Zhengzhong Shao5Xin Chen6Yuehua Liu7Department of Orthodontics, Shanghai Stomatological Hospital & School of Stomatology, Department of Macromolecular Science, Fudan University, Shanghai 200433, ChinaDepartment of Orthodontics, Shanghai Stomatological Hospital & School of Stomatology, Department of Macromolecular Science, Fudan University, Shanghai 200433, ChinaDepartment of Orthodontics, Shanghai Stomatological Hospital & School of Stomatology, Department of Macromolecular Science, Fudan University, Shanghai 200433, ChinaDepartment of Orthodontics, Shanghai Stomatological Hospital & School of Stomatology, Department of Macromolecular Science, Fudan University, Shanghai 200433, ChinaDepartment of Orthodontics, Shanghai Stomatological Hospital & School of Stomatology, Department of Macromolecular Science, Fudan University, Shanghai 200433, ChinaDepartment of Orthodontics, Shanghai Stomatological Hospital & School of Stomatology, Department of Macromolecular Science, Fudan University, Shanghai 200433, ChinaDepartment of Orthodontics, Shanghai Stomatological Hospital & School of Stomatology, Department of Macromolecular Science, Fudan University, Shanghai 200433, ChinaDepartment of Orthodontics, Shanghai Stomatological Hospital & School of Stomatology, Department of Macromolecular Science, Fudan University, Shanghai 200433, ChinaSilk fibroin is regarded as a promising biomaterial in various areas, including bone tissue regeneration. Herein, Laponite<sup>®</sup> (LAP), which can promote osteogenic differentiation, was introduced into regenerated silk fibroin (RSF) to prepare an RSF/LAP hybrid hydrogel. This thixotropic hydrogel is injectable during the operation process, which is favorable for repairing bone defects. Our previous work demonstrated that the RSF/LAP hydrogel greatly promoted the osteogenic differentiation of osteoblasts in vitro. In the present study, the RSF/LAP hydrogel was found to have excellent biocompatibility and significantly improved new bone formation in a standard rat calvarial defect model in vivo. Additionally, the underlying biological mechanism of the RSF/LAP hydrogel in promoting osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) was extensively explored. The results indicate that the RSF/LAP hydrogels provide suitable conditions for the adhesion and proliferation of BMSCs, showing good biocompatibility in vitro. With the increase in LAP content, the alkaline phosphatase (ALP) activity and mRNA and protein expression of the osteogenic markers of BMSCs improved significantly. Protein kinase B (AKT) pathway activation was found to be responsible for the inherent osteogenic properties of the RSF/LAP hybrid hydrogel. Therefore, the results shown in this study firmly suggest such an injectable RSF/LAP hydrogel with good biocompatibility (both in vitro and in vivo) would have good application prospects in the field of bone regeneration.https://www.mdpi.com/2079-4983/14/2/86silk fibroinnanoparticlesinjectable hydrogelbone regenerationosteogenesis |
| spellingShingle | Liangyan Sun Minqi Lu Ling Chen Bingjiao Zhao Jinrong Yao Zhengzhong Shao Xin Chen Yuehua Liu Silk–Inorganic Nanoparticle Hybrid Hydrogel as an Injectable Bone Repairing Biomaterial Journal of Functional Biomaterials silk fibroin nanoparticles injectable hydrogel bone regeneration osteogenesis |
| title | Silk–Inorganic Nanoparticle Hybrid Hydrogel as an Injectable Bone Repairing Biomaterial |
| title_full | Silk–Inorganic Nanoparticle Hybrid Hydrogel as an Injectable Bone Repairing Biomaterial |
| title_fullStr | Silk–Inorganic Nanoparticle Hybrid Hydrogel as an Injectable Bone Repairing Biomaterial |
| title_full_unstemmed | Silk–Inorganic Nanoparticle Hybrid Hydrogel as an Injectable Bone Repairing Biomaterial |
| title_short | Silk–Inorganic Nanoparticle Hybrid Hydrogel as an Injectable Bone Repairing Biomaterial |
| title_sort | silk inorganic nanoparticle hybrid hydrogel as an injectable bone repairing biomaterial |
| topic | silk fibroin nanoparticles injectable hydrogel bone regeneration osteogenesis |
| url | https://www.mdpi.com/2079-4983/14/2/86 |
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