Analyses of <i>Leishmania</i>-LRV Co-Phylogenetic Patterns and Evolutionary Variability of Viral Proteins
<i>Leishmania</i> spp. are important pathogens causing a vector-borne disease with a broad range of clinical manifestations from self-healing ulcers to the life-threatening visceral forms. Presence of <i>Leishmania RNA virus</i> (LRV) confers survival advantage to these paras...
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MDPI AG
2021-11-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/13/11/2305 |
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| author | Alexei Y. Kostygov Danyil Grybchuk Yulia Kleschenko Daniil S. Chistyakov Alexander N. Lukashev Evgeny S. Gerasimov Vyacheslav Yurchenko |
| author_facet | Alexei Y. Kostygov Danyil Grybchuk Yulia Kleschenko Daniil S. Chistyakov Alexander N. Lukashev Evgeny S. Gerasimov Vyacheslav Yurchenko |
| author_sort | Alexei Y. Kostygov |
| collection | DOAJ |
| description | <i>Leishmania</i> spp. are important pathogens causing a vector-borne disease with a broad range of clinical manifestations from self-healing ulcers to the life-threatening visceral forms. Presence of <i>Leishmania RNA virus</i> (LRV) confers survival advantage to these parasites by suppressing anti-leishmanial immunity in the vertebrate host. The two viral species, LRV1 and LRV2 infect species of the subgenera <i>Viannia and Leishmania,</i> respectively. In this work we investigated co-phylogenetic patterns of leishmaniae and their viruses on a small scale (LRV2 in <i>L. major</i>) and demonstrated their predominant coevolution, occasionally broken by intraspecific host switches. Our analysis of the two viral genes, encoding the capsid and RNA-dependent RNA polymerase (RDRP), revealed them to be under the pressure of purifying selection, which was considerably stronger for the former gene across the whole tree. The selective pressure also differs between the LRV clades and correlates with the frequency of interspecific host switches. In addition, using experimental (capsid) and predicted (RDRP) models we demonstrated that the evolutionary variability across the structure is strikingly different in these two viral proteins. |
| first_indexed | 2024-03-10T04:58:58Z |
| format | Article |
| id | doaj.art-3ae7c7acd0394007bbb285053d753acf |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T04:58:58Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-3ae7c7acd0394007bbb285053d753acf2023-11-23T01:58:30ZengMDPI AGViruses1999-49152021-11-011311230510.3390/v13112305Analyses of <i>Leishmania</i>-LRV Co-Phylogenetic Patterns and Evolutionary Variability of Viral ProteinsAlexei Y. Kostygov0Danyil Grybchuk1Yulia Kleschenko2Daniil S. Chistyakov3Alexander N. Lukashev4Evgeny S. Gerasimov5Vyacheslav Yurchenko6Life Science Research Centre, Faculty of Science, University of Ostrava, 71000 Ostrava, Czech RepublicLife Science Research Centre, Faculty of Science, University of Ostrava, 71000 Ostrava, Czech RepublicMartsinovsky Institute of Medical Parasitology, Sechenov University, 119435 Moscow, RussiaMartsinovsky Institute of Medical Parasitology, Sechenov University, 119435 Moscow, RussiaMartsinovsky Institute of Medical Parasitology, Sechenov University, 119435 Moscow, RussiaFaculty of Biology, M.V. Lomonosov Moscow State University, 119991 Moscow, RussiaLife Science Research Centre, Faculty of Science, University of Ostrava, 71000 Ostrava, Czech Republic<i>Leishmania</i> spp. are important pathogens causing a vector-borne disease with a broad range of clinical manifestations from self-healing ulcers to the life-threatening visceral forms. Presence of <i>Leishmania RNA virus</i> (LRV) confers survival advantage to these parasites by suppressing anti-leishmanial immunity in the vertebrate host. The two viral species, LRV1 and LRV2 infect species of the subgenera <i>Viannia and Leishmania,</i> respectively. In this work we investigated co-phylogenetic patterns of leishmaniae and their viruses on a small scale (LRV2 in <i>L. major</i>) and demonstrated their predominant coevolution, occasionally broken by intraspecific host switches. Our analysis of the two viral genes, encoding the capsid and RNA-dependent RNA polymerase (RDRP), revealed them to be under the pressure of purifying selection, which was considerably stronger for the former gene across the whole tree. The selective pressure also differs between the LRV clades and correlates with the frequency of interspecific host switches. In addition, using experimental (capsid) and predicted (RDRP) models we demonstrated that the evolutionary variability across the structure is strikingly different in these two viral proteins.https://www.mdpi.com/1999-4915/13/11/2305<i>Leishmaniavirus</i>coevolutionphylogenomics |
| spellingShingle | Alexei Y. Kostygov Danyil Grybchuk Yulia Kleschenko Daniil S. Chistyakov Alexander N. Lukashev Evgeny S. Gerasimov Vyacheslav Yurchenko Analyses of <i>Leishmania</i>-LRV Co-Phylogenetic Patterns and Evolutionary Variability of Viral Proteins Viruses <i>Leishmaniavirus</i> coevolution phylogenomics |
| title | Analyses of <i>Leishmania</i>-LRV Co-Phylogenetic Patterns and Evolutionary Variability of Viral Proteins |
| title_full | Analyses of <i>Leishmania</i>-LRV Co-Phylogenetic Patterns and Evolutionary Variability of Viral Proteins |
| title_fullStr | Analyses of <i>Leishmania</i>-LRV Co-Phylogenetic Patterns and Evolutionary Variability of Viral Proteins |
| title_full_unstemmed | Analyses of <i>Leishmania</i>-LRV Co-Phylogenetic Patterns and Evolutionary Variability of Viral Proteins |
| title_short | Analyses of <i>Leishmania</i>-LRV Co-Phylogenetic Patterns and Evolutionary Variability of Viral Proteins |
| title_sort | analyses of i leishmania i lrv co phylogenetic patterns and evolutionary variability of viral proteins |
| topic | <i>Leishmaniavirus</i> coevolution phylogenomics |
| url | https://www.mdpi.com/1999-4915/13/11/2305 |
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