Upcycling of poly(ethylene terephthalate) to produce high-value bio-products
Summary: More than 70 million tons of poly(ethylene terephthalate) (PET) are manufactured worldwide every year. The accumulation of PET waste has become a global pollution concern, motivating the urgent development of technologies to valorize post-consumer PET. The development of chemocatalytic and...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-01-01
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| Series: | Cell Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124722018071 |
| _version_ | 1797961105924947968 |
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| author | Jinjin Diao Yifeng Hu Yuxin Tian Rhiannon Carr Tae Seok Moon |
| author_facet | Jinjin Diao Yifeng Hu Yuxin Tian Rhiannon Carr Tae Seok Moon |
| author_sort | Jinjin Diao |
| collection | DOAJ |
| description | Summary: More than 70 million tons of poly(ethylene terephthalate) (PET) are manufactured worldwide every year. The accumulation of PET waste has become a global pollution concern, motivating the urgent development of technologies to valorize post-consumer PET. The development of chemocatalytic and enzymatic approaches for depolymerizing PET to its corresponding monomers opens up new opportunities for PET upcycling through biological transformation. Here, we identify Rhodococcus jostii strain PET (RPET) that can directly use PET hydrolysate as a sole carbon source. We also investigate the potential of RPET to upcycle PET into value-added chemicals, using lycopene as a proof-of-concept product. Through rational metabolic engineering, we improve lycopene production by more than 500-fold over that of the wild type. In addition, we demonstrate the production of approximately 1,300 μg/L lycopene from PET by cascading this strain with PET alkaline hydrolysis. This work highlights the great potential of biological conversion as a means of achieving PET upcycling. |
| first_indexed | 2024-04-11T00:54:13Z |
| format | Article |
| id | doaj.art-3ae8fb0a3bb142afb2533a3104d21c49 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-04-11T00:54:13Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-3ae8fb0a3bb142afb2533a3104d21c492023-01-05T06:23:58ZengElsevierCell Reports2211-12472023-01-01421111908Upcycling of poly(ethylene terephthalate) to produce high-value bio-productsJinjin Diao0Yifeng Hu1Yuxin Tian2Rhiannon Carr3Tae Seok Moon4Department of Energy, Environmental and Chemical Engineering, Washington University in St. Louis, One Brookings Drive, Box 1180, St. Louis, MO 63130, USADepartment of Energy, Environmental and Chemical Engineering, Washington University in St. Louis, One Brookings Drive, Box 1180, St. Louis, MO 63130, USADepartment of Energy, Environmental and Chemical Engineering, Washington University in St. Louis, One Brookings Drive, Box 1180, St. Louis, MO 63130, USADepartment of Energy, Environmental and Chemical Engineering, Washington University in St. Louis, One Brookings Drive, Box 1180, St. Louis, MO 63130, USADepartment of Energy, Environmental and Chemical Engineering, Washington University in St. Louis, One Brookings Drive, Box 1180, St. Louis, MO 63130, USA; Division of Biology and Biomedical Sciences, Washington University in St. Louis, One Brookings Drive, Box 1180, St. Louis, MO 63130, USA; Corresponding authorSummary: More than 70 million tons of poly(ethylene terephthalate) (PET) are manufactured worldwide every year. The accumulation of PET waste has become a global pollution concern, motivating the urgent development of technologies to valorize post-consumer PET. The development of chemocatalytic and enzymatic approaches for depolymerizing PET to its corresponding monomers opens up new opportunities for PET upcycling through biological transformation. Here, we identify Rhodococcus jostii strain PET (RPET) that can directly use PET hydrolysate as a sole carbon source. We also investigate the potential of RPET to upcycle PET into value-added chemicals, using lycopene as a proof-of-concept product. Through rational metabolic engineering, we improve lycopene production by more than 500-fold over that of the wild type. In addition, we demonstrate the production of approximately 1,300 μg/L lycopene from PET by cascading this strain with PET alkaline hydrolysis. This work highlights the great potential of biological conversion as a means of achieving PET upcycling.http://www.sciencedirect.com/science/article/pii/S2211124722018071CP: Microbiology |
| spellingShingle | Jinjin Diao Yifeng Hu Yuxin Tian Rhiannon Carr Tae Seok Moon Upcycling of poly(ethylene terephthalate) to produce high-value bio-products Cell Reports CP: Microbiology |
| title | Upcycling of poly(ethylene terephthalate) to produce high-value bio-products |
| title_full | Upcycling of poly(ethylene terephthalate) to produce high-value bio-products |
| title_fullStr | Upcycling of poly(ethylene terephthalate) to produce high-value bio-products |
| title_full_unstemmed | Upcycling of poly(ethylene terephthalate) to produce high-value bio-products |
| title_short | Upcycling of poly(ethylene terephthalate) to produce high-value bio-products |
| title_sort | upcycling of poly ethylene terephthalate to produce high value bio products |
| topic | CP: Microbiology |
| url | http://www.sciencedirect.com/science/article/pii/S2211124722018071 |
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