A Novel Genotype and First Record of <i>Trypanosoma lainsoni</i> in Argentina
Trypanosomes are a group of parasitic flagellates with medical and veterinary importance. Despite many species having been described in this genus, little is known about many of them. Here, we report a genetic and morphological characterization of trypanosomatids isolated from wild mammals from the...
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MDPI AG
2020-09-01
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| Series: | Pathogens |
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| author | Anahí G. Díaz Paula G. Ragone Fanny Rusman Noelia Floridia-Yapur Rubén M. Barquez M. Mónica Díaz Nicolás Tomasini Patricio Diosque |
| author_facet | Anahí G. Díaz Paula G. Ragone Fanny Rusman Noelia Floridia-Yapur Rubén M. Barquez M. Mónica Díaz Nicolás Tomasini Patricio Diosque |
| author_sort | Anahí G. Díaz |
| collection | DOAJ |
| description | Trypanosomes are a group of parasitic flagellates with medical and veterinary importance. Despite many species having been described in this genus, little is known about many of them. Here, we report a genetic and morphological characterization of trypanosomatids isolated from wild mammals from the Argentine Chaco region. Parasites were morphologically and ultrastructurally characterized by light microscopy and transmission electron microscopy. Additionally, 18s rRNA and gGAPDH genes were sequenced and analyzed using maximum likelihood and Bayesian inference. Morphological characterization showed clear characteristics associated with the <i>Trypanosoma</i> genus. The genetic characterization demonstrates that the studied isolates have identical sequences and a pairwise identity of 99% with <i>Trypanosoma lainsoni</i>, which belongs to the clade of lizards and snakes/rodents and marsupials. To date, this species had only been found in the Amazon region. Our finding represents the second report of <i>T. lainsoni</i> and the first record for the Chaco region. Furthermore, we ultrastructurally described for the first time the species. Finally, the host range of <i>T. lainsoni</i> was expanded (<i>Leopardus geoffroyi,</i> Carenivora, Felidae; and <i>Calomys</i> sp., Rodentia, Cricetidae), showing a wide host range for this species. |
| first_indexed | 2024-03-10T16:32:39Z |
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| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T16:32:39Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-3ae905a179034dd98abf399de9e709622023-11-20T12:40:12ZengMDPI AGPathogens2076-08172020-09-019973110.3390/pathogens9090731A Novel Genotype and First Record of <i>Trypanosoma lainsoni</i> in ArgentinaAnahí G. Díaz0Paula G. Ragone1Fanny Rusman2Noelia Floridia-Yapur3Rubén M. Barquez4M. Mónica Díaz5Nicolás Tomasini6Patricio Diosque7Unidad de Epidemiología Molecular (UEM), Instituto de Patología Experimental, Universidad Nacional de Salta-CONICET, 4400 Salta, Salta, ArgentinaUnidad de Epidemiología Molecular (UEM), Instituto de Patología Experimental, Universidad Nacional de Salta-CONICET, 4400 Salta, Salta, ArgentinaUnidad de Epidemiología Molecular (UEM), Instituto de Patología Experimental, Universidad Nacional de Salta-CONICET, 4400 Salta, Salta, ArgentinaUnidad de Epidemiología Molecular (UEM), Instituto de Patología Experimental, Universidad Nacional de Salta-CONICET, 4400 Salta, Salta, ArgentinaPrograma de Investigación de Biodiversidad Argentina (PIDBA), Facultad de Ciencias Naturales e Instituto Miguel Lillo, Universidad Nacional de Tucumán-CONICET, 4000 San Miguel de Tucumán, Tucumán, ArgentinaPrograma de Investigación de Biodiversidad Argentina (PIDBA), Facultad de Ciencias Naturales e Instituto Miguel Lillo, Universidad Nacional de Tucumán-CONICET, 4000 San Miguel de Tucumán, Tucumán, ArgentinaUnidad de Epidemiología Molecular (UEM), Instituto de Patología Experimental, Universidad Nacional de Salta-CONICET, 4400 Salta, Salta, ArgentinaUnidad de Epidemiología Molecular (UEM), Instituto de Patología Experimental, Universidad Nacional de Salta-CONICET, 4400 Salta, Salta, ArgentinaTrypanosomes are a group of parasitic flagellates with medical and veterinary importance. Despite many species having been described in this genus, little is known about many of them. Here, we report a genetic and morphological characterization of trypanosomatids isolated from wild mammals from the Argentine Chaco region. Parasites were morphologically and ultrastructurally characterized by light microscopy and transmission electron microscopy. Additionally, 18s rRNA and gGAPDH genes were sequenced and analyzed using maximum likelihood and Bayesian inference. Morphological characterization showed clear characteristics associated with the <i>Trypanosoma</i> genus. The genetic characterization demonstrates that the studied isolates have identical sequences and a pairwise identity of 99% with <i>Trypanosoma lainsoni</i>, which belongs to the clade of lizards and snakes/rodents and marsupials. To date, this species had only been found in the Amazon region. Our finding represents the second report of <i>T. lainsoni</i> and the first record for the Chaco region. Furthermore, we ultrastructurally described for the first time the species. Finally, the host range of <i>T. lainsoni</i> was expanded (<i>Leopardus geoffroyi,</i> Carenivora, Felidae; and <i>Calomys</i> sp., Rodentia, Cricetidae), showing a wide host range for this species.https://www.mdpi.com/2076-0817/9/9/731<i>Trypanosoma lainsoni</i><i>Leopardus geoffroyi</i><i>Calomys</i> spp.18S rDNA genesgGAPDH genesmorphology |
| spellingShingle | Anahí G. Díaz Paula G. Ragone Fanny Rusman Noelia Floridia-Yapur Rubén M. Barquez M. Mónica Díaz Nicolás Tomasini Patricio Diosque A Novel Genotype and First Record of <i>Trypanosoma lainsoni</i> in Argentina Pathogens <i>Trypanosoma lainsoni</i> <i>Leopardus geoffroyi</i> <i>Calomys</i> spp. 18S rDNA genes gGAPDH genes morphology |
| title | A Novel Genotype and First Record of <i>Trypanosoma lainsoni</i> in Argentina |
| title_full | A Novel Genotype and First Record of <i>Trypanosoma lainsoni</i> in Argentina |
| title_fullStr | A Novel Genotype and First Record of <i>Trypanosoma lainsoni</i> in Argentina |
| title_full_unstemmed | A Novel Genotype and First Record of <i>Trypanosoma lainsoni</i> in Argentina |
| title_short | A Novel Genotype and First Record of <i>Trypanosoma lainsoni</i> in Argentina |
| title_sort | novel genotype and first record of i trypanosoma lainsoni i in argentina |
| topic | <i>Trypanosoma lainsoni</i> <i>Leopardus geoffroyi</i> <i>Calomys</i> spp. 18S rDNA genes gGAPDH genes morphology |
| url | https://www.mdpi.com/2076-0817/9/9/731 |
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