The change of metabotropic glutamate receptor 5 expression level in rats with late-stage traumatic brain injury and the therapeutic effect of taurine
<p><strong>Objective </strong> To investigate the change of metabotropic glutamate receptor 5 (mGluR5) expression level in rats with late-stage (the 7th day) traumatic brain injury (TBI) and the role of taurine.<strong> Methods</strong> The left cerebral TBI rat model...
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| Format: | Article |
| Language: | English |
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Tianjin Huanhu Hospital
2016-08-01
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| Series: | Chinese Journal of Contemporary Neurology and Neurosurgery |
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| Online Access: | http://www.cjcnn.org/index.php/cjcnn/article/view/1443 |
| _version_ | 1811319215191228416 |
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| author | Ying CAI Hui-ling HUANG Wei-jia FAN Qiao-li WU |
| author_facet | Ying CAI Hui-ling HUANG Wei-jia FAN Qiao-li WU |
| author_sort | Ying CAI |
| collection | DOAJ |
| description | <p><strong>Objective </strong> To investigate the change of metabotropic glutamate receptor 5 (mGluR5) expression level in rats with late-stage (the 7th day) traumatic brain injury (TBI) and the role of taurine.<strong> Methods</strong> The left cerebral TBI rat models were made by using lateral fluid percussion method. A total of 30 specific pathogen free (SPF) male Sprague-Dawley (SD) rats were randomly divided into 3 groups: sham operation group (control group), TBI model group (TBI group) and taurine treatment group (taurine group). Wet and dry weight method was used to measure the brain water content. Real-time fluorescent quantitative polymerase chain reaction (PCR) and Western blotting were used to detect the change of mRNA and protein expression of aquaporin 4 (AQP4) and mGluR5 in each group. <strong>Results</strong> Compared with control group, the brain water content (<em>t</em> = 4.893, <em>P</em> = 0.002), AQP4 mRNA (<em>t</em> = 6.523, <em>P</em> = 0.000) and protein (<em>t</em> = 4.366, <em>P</em> = 0.008) expression were upregulated, while mGluR5 mRNA (<em>t</em> = 5.776, <em>P</em> = 0.001) and protein (<em>t</em> = 3.945, <em>P</em> = 0.014) expression were downregulated in TBI group. After taurine treatment, the brain water content (<em>t</em> = 2.151, <em>P</em> = 0.140), AQP4 mRNA (<em>t</em> = 1.144，<em>P</em> = 0.432) and protein (<em>t</em> = 0.367, <em>P</em> = 0.804) decreased to normal, while mGluR5 mRNA (<em>t</em> = 1.824, <em>P</em> = 0.216) and protein (<em>t</em> = 1.185, <em>P</em> = 0.414) increased to normal. Correlation analysis showed brain water content was negatively correlated with mGluR5 mRNA (<em>r</em> = -0.617, <em>P</em> = 0.014) and mGluR5 protein (<em>r</em> = -0.665, <em>P</em> = 0.007), while it was positively correlated with AQP4 protein (<em>r</em> = 0.658, <em>P</em> = 0.008). <strong>Conclusions</strong> Taurine can significantly increase the mGluR5 expression level of brain issue in the late-stage (the 7th day) of TBI and decline brain edema and brain water content. It may be a potential protective agent as an inhibitory neurotransmitter.</p><p> </p><p><strong>DOI: </strong>10.3969/j.issn.1672-6731.2016.08.008</p> |
| first_indexed | 2024-04-13T12:37:55Z |
| format | Article |
| id | doaj.art-3ae92cc74ae448409642ee7f88468e7c |
| institution | Directory Open Access Journal |
| issn | 1672-6731 |
| language | English |
| last_indexed | 2024-04-13T12:37:55Z |
| publishDate | 2016-08-01 |
| publisher | Tianjin Huanhu Hospital |
| record_format | Article |
| series | Chinese Journal of Contemporary Neurology and Neurosurgery |
| spelling | doaj.art-3ae92cc74ae448409642ee7f88468e7c2022-12-22T02:46:35ZengTianjin Huanhu HospitalChinese Journal of Contemporary Neurology and Neurosurgery1672-67312016-08-011685035081421The change of metabotropic glutamate receptor 5 expression level in rats with late-stage traumatic brain injury and the therapeutic effect of taurineYing CAI0Hui-ling HUANG1Wei-jia FAN2Qiao-li WU3Tianjin Huanhu Hospital; Tianjin Neurosurgery Institute; Tianjin Key Laboratory of Cerebrovascular and Neurodegenerative Diseases, Tianjin 300350, ChinaTianjin Huanhu Hospital; Tianjin Neurosurgery Institute; Tianjin Key Laboratory of Cerebrovascular and Neurodegenerative Diseases, Tianjin 300350, ChinaTianjin Huanhu Hospital; Tianjin Neurosurgery Institute; Tianjin Key Laboratory of Cerebrovascular and Neurodegenerative Diseases, Tianjin 300350, ChinaTianjin Huanhu Hospital; Tianjin Neurosurgery Institute; Tianjin Key Laboratory of Cerebrovascular and Neurodegenerative Diseases, Tianjin 300350, China<p><strong>Objective </strong> To investigate the change of metabotropic glutamate receptor 5 (mGluR5) expression level in rats with late-stage (the 7th day) traumatic brain injury (TBI) and the role of taurine.<strong> Methods</strong> The left cerebral TBI rat models were made by using lateral fluid percussion method. A total of 30 specific pathogen free (SPF) male Sprague-Dawley (SD) rats were randomly divided into 3 groups: sham operation group (control group), TBI model group (TBI group) and taurine treatment group (taurine group). Wet and dry weight method was used to measure the brain water content. Real-time fluorescent quantitative polymerase chain reaction (PCR) and Western blotting were used to detect the change of mRNA and protein expression of aquaporin 4 (AQP4) and mGluR5 in each group. <strong>Results</strong> Compared with control group, the brain water content (<em>t</em> = 4.893, <em>P</em> = 0.002), AQP4 mRNA (<em>t</em> = 6.523, <em>P</em> = 0.000) and protein (<em>t</em> = 4.366, <em>P</em> = 0.008) expression were upregulated, while mGluR5 mRNA (<em>t</em> = 5.776, <em>P</em> = 0.001) and protein (<em>t</em> = 3.945, <em>P</em> = 0.014) expression were downregulated in TBI group. After taurine treatment, the brain water content (<em>t</em> = 2.151, <em>P</em> = 0.140), AQP4 mRNA (<em>t</em> = 1.144，<em>P</em> = 0.432) and protein (<em>t</em> = 0.367, <em>P</em> = 0.804) decreased to normal, while mGluR5 mRNA (<em>t</em> = 1.824, <em>P</em> = 0.216) and protein (<em>t</em> = 1.185, <em>P</em> = 0.414) increased to normal. Correlation analysis showed brain water content was negatively correlated with mGluR5 mRNA (<em>r</em> = -0.617, <em>P</em> = 0.014) and mGluR5 protein (<em>r</em> = -0.665, <em>P</em> = 0.007), while it was positively correlated with AQP4 protein (<em>r</em> = 0.658, <em>P</em> = 0.008). <strong>Conclusions</strong> Taurine can significantly increase the mGluR5 expression level of brain issue in the late-stage (the 7th day) of TBI and decline brain edema and brain water content. It may be a potential protective agent as an inhibitory neurotransmitter.</p><p> </p><p><strong>DOI: </strong>10.3969/j.issn.1672-6731.2016.08.008</p>http://www.cjcnn.org/index.php/cjcnn/article/view/1443Craniocerebral traumaReceptors, glutamateTaurineDisease models, animal |
| spellingShingle | Ying CAI Hui-ling HUANG Wei-jia FAN Qiao-li WU The change of metabotropic glutamate receptor 5 expression level in rats with late-stage traumatic brain injury and the therapeutic effect of taurine Chinese Journal of Contemporary Neurology and Neurosurgery Craniocerebral trauma Receptors, glutamate Taurine Disease models, animal |
| title | The change of metabotropic glutamate receptor 5 expression level in rats with late-stage traumatic brain injury and the therapeutic effect of taurine |
| title_full | The change of metabotropic glutamate receptor 5 expression level in rats with late-stage traumatic brain injury and the therapeutic effect of taurine |
| title_fullStr | The change of metabotropic glutamate receptor 5 expression level in rats with late-stage traumatic brain injury and the therapeutic effect of taurine |
| title_full_unstemmed | The change of metabotropic glutamate receptor 5 expression level in rats with late-stage traumatic brain injury and the therapeutic effect of taurine |
| title_short | The change of metabotropic glutamate receptor 5 expression level in rats with late-stage traumatic brain injury and the therapeutic effect of taurine |
| title_sort | change of metabotropic glutamate receptor 5 expression level in rats with late stage traumatic brain injury and the therapeutic effect of taurine |
| topic | Craniocerebral trauma Receptors, glutamate Taurine Disease models, animal |
| url | http://www.cjcnn.org/index.php/cjcnn/article/view/1443 |
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