Molecular Identification of Mudskipper Fish (<i>Periophthalmus</i> spp.) from Baros Beach, Bantul, Yogyakarta
Mudskipper fish is amphibious fish belonging to the family Gobiidae. Coastal communities widely consume mudskipper to meet their animal protein needs. Mudskipper is primarily cryptic species that are morphologically difficult to identify and distinguish from other mudskipper fish species. Consequent...
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| Format: | Article |
| Language: | English |
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Universitas Gadjah Mada
2021-10-01
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| Series: | Journal of Tropical Biodiversity and Biotechnology |
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| Online Access: | https://jurnal.ugm.ac.id/jtbb/article/view/66391 |
| _version_ | 1818918316229525504 |
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| author | Katon Waskito Aji Tuty Arisuryanti |
| author_facet | Katon Waskito Aji Tuty Arisuryanti |
| author_sort | Katon Waskito Aji |
| collection | DOAJ |
| description | Mudskipper fish is amphibious fish belonging to the family Gobiidae. Coastal communities widely consume mudskipper to meet their animal protein needs. Mudskipper is primarily cryptic species that are morphologically difficult to identify and distinguish from other mudskipper fish species. Consequently, it can be confused with the naming of mudskipper fish species and can affect the conservation efforts of the fish in their habitat. One of the molecular approaches that can be used to identify the fish species quickly and accurately is DNA barcoding using the COI mitochondrial gene. However, the research on the identification of mudskipper fish in Indonesia is still very limited. Therefore, this study aimed to identify 26 mudskipper fish from Baros Beach, Bantul, Yogyakarta, using COI mitochondrial gene as a molecular marker for DNA barcoding. The method used in this study was a PCR method with universal primers, FishF2 and FishR2. The data obtained were then analyzed using GeneStudio, DNASTAR, BLAST, Identification Engine, Mesquite, MEGAX, and BEAST. The analysis was conducted to obtain similarity, genetic distance and reconstruct a phylogenetic tree. The result revealed that all 26 samples of mudskippers collected from Baros Beach were identified in one genus, namely Periophthalmus, and consisted of 3 species, namely P. kalolo (16 samples), P. argentilineatus (9 samples), and P. novemradiatus (1 sample). Furthermore, this study also discovered a suspected cryptic species in P. argentilineatus with a genetic distance of 5.46-5.96% between clade E, F compared with clade G. Further morphological studies are needed to confirm the species status of these three clades before solidly proclaim that they are cryptic species. |
| first_indexed | 2024-12-20T00:48:01Z |
| format | Article |
| id | doaj.art-3aeacdca84c64dfd806cdb90350b9008 |
| institution | Directory Open Access Journal |
| issn | 2540-9573 2540-9581 |
| language | English |
| last_indexed | 2024-12-20T00:48:01Z |
| publishDate | 2021-10-01 |
| publisher | Universitas Gadjah Mada |
| record_format | Article |
| series | Journal of Tropical Biodiversity and Biotechnology |
| spelling | doaj.art-3aeacdca84c64dfd806cdb90350b90082022-12-21T19:59:21ZengUniversitas Gadjah MadaJournal of Tropical Biodiversity and Biotechnology2540-95732540-95812021-10-016310.22146/jtbb.6639130690Molecular Identification of Mudskipper Fish (<i>Periophthalmus</i> spp.) from Baros Beach, Bantul, YogyakartaKaton Waskito Aji0Tuty Arisuryanti1Laboratory of Genetics and Breeding, Faculty of Biology, Universitas Gadjah MadaLaboratory of Genetics and Breeding, Faculty of Biology, Universitas Gadjah MadaMudskipper fish is amphibious fish belonging to the family Gobiidae. Coastal communities widely consume mudskipper to meet their animal protein needs. Mudskipper is primarily cryptic species that are morphologically difficult to identify and distinguish from other mudskipper fish species. Consequently, it can be confused with the naming of mudskipper fish species and can affect the conservation efforts of the fish in their habitat. One of the molecular approaches that can be used to identify the fish species quickly and accurately is DNA barcoding using the COI mitochondrial gene. However, the research on the identification of mudskipper fish in Indonesia is still very limited. Therefore, this study aimed to identify 26 mudskipper fish from Baros Beach, Bantul, Yogyakarta, using COI mitochondrial gene as a molecular marker for DNA barcoding. The method used in this study was a PCR method with universal primers, FishF2 and FishR2. The data obtained were then analyzed using GeneStudio, DNASTAR, BLAST, Identification Engine, Mesquite, MEGAX, and BEAST. The analysis was conducted to obtain similarity, genetic distance and reconstruct a phylogenetic tree. The result revealed that all 26 samples of mudskippers collected from Baros Beach were identified in one genus, namely Periophthalmus, and consisted of 3 species, namely P. kalolo (16 samples), P. argentilineatus (9 samples), and P. novemradiatus (1 sample). Furthermore, this study also discovered a suspected cryptic species in P. argentilineatus with a genetic distance of 5.46-5.96% between clade E, F compared with clade G. Further morphological studies are needed to confirm the species status of these three clades before solidly proclaim that they are cryptic species.https://jurnal.ugm.ac.id/jtbb/article/view/66391coicryptic speciesdna barcodingmudskipper |
| spellingShingle | Katon Waskito Aji Tuty Arisuryanti Molecular Identification of Mudskipper Fish (<i>Periophthalmus</i> spp.) from Baros Beach, Bantul, Yogyakarta Journal of Tropical Biodiversity and Biotechnology coi cryptic species dna barcoding mudskipper |
| title | Molecular Identification of Mudskipper Fish (<i>Periophthalmus</i> spp.) from Baros Beach, Bantul, Yogyakarta |
| title_full | Molecular Identification of Mudskipper Fish (<i>Periophthalmus</i> spp.) from Baros Beach, Bantul, Yogyakarta |
| title_fullStr | Molecular Identification of Mudskipper Fish (<i>Periophthalmus</i> spp.) from Baros Beach, Bantul, Yogyakarta |
| title_full_unstemmed | Molecular Identification of Mudskipper Fish (<i>Periophthalmus</i> spp.) from Baros Beach, Bantul, Yogyakarta |
| title_short | Molecular Identification of Mudskipper Fish (<i>Periophthalmus</i> spp.) from Baros Beach, Bantul, Yogyakarta |
| title_sort | molecular identification of mudskipper fish i periophthalmus i spp from baros beach bantul yogyakarta |
| topic | coi cryptic species dna barcoding mudskipper |
| url | https://jurnal.ugm.ac.id/jtbb/article/view/66391 |
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