Chitosan Inhibits <i>Helicobacter pylori</i> Growth and Urease Production and Prevents Its Infection of Human Gastric Carcinoma Cells
This study investigated the effects of shrimp chitosan with 95% degree of deacetylation (DD95) in combination with clinical antibiotics on the growth and urease production of <i>Helicobacter pylori</i>. The inhibitory effect of DD95 on the adherence of <i>H. pylori</i> to the...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-10-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/18/11/542 |
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| author | Shun-Hsien Chang Pei-Ling Hsieh Guo-Jane Tsai |
| author_facet | Shun-Hsien Chang Pei-Ling Hsieh Guo-Jane Tsai |
| author_sort | Shun-Hsien Chang |
| collection | DOAJ |
| description | This study investigated the effects of shrimp chitosan with 95% degree of deacetylation (DD95) in combination with clinical antibiotics on the growth and urease production of <i>Helicobacter pylori</i>. The inhibitory effect of DD95 on the adherence of <i>H. pylori</i> to the human intestinal carcinoma cells (TSGH9201) was also investigated. Five strains of <i>H. pylori</i>, including three standard strains and two strains of clinical isolates were used as the test strains. The inhibitory effects of DD95 on growth and urease production of various strains of <i>H. pylori</i> increased with increasing DD95 concentration and decreasing pH values from pH 6.0 to pH 2.0. Urease activity of <i>H. pylori</i> at pH 2.0 in the presence of 4000 μg/mL of DD95 decreased by 37.86% to 46.53%. In the presence of 50 μg/mL antibiotics of amoxicillin, tetracycline, or metronidazole at pH 6.0 and pH 2.0, <i>H. pylori</i> counts were decreased by 1.51–3.19, and 1.47–2.82 Log CFU/mL, respectively. Following the addition of 4000 μg/mL DD95 into the 50 μg/mL antibiotic-containing culture medium with pH 6.0 and pH 2.0, overall <i>H. pylori</i> counts were strongly decreased by 3.67–7.61 and 6.61–6.70 Log CFU/mL, respectively. Further, DD95 could inhibit the adherence of <i>H. pylori</i> on TSGH 9201 cells, as evidenced by fluorescent microscopy and thus may potentially protect against <i>H. pylori</i> infection. |
| first_indexed | 2024-03-10T15:14:41Z |
| format | Article |
| id | doaj.art-3aeba5101f3048c9b7a72a4906eb7881 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-10T15:14:41Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-3aeba5101f3048c9b7a72a4906eb78812023-11-20T19:02:24ZengMDPI AGMarine Drugs1660-33972020-10-01181154210.3390/md18110542Chitosan Inhibits <i>Helicobacter pylori</i> Growth and Urease Production and Prevents Its Infection of Human Gastric Carcinoma CellsShun-Hsien Chang0Pei-Ling Hsieh1Guo-Jane Tsai2Institute of Food Safety and Risk Management, National Taiwan Ocean University, Keelung 20224, TaiwanDepartment of Food Science, National Taiwan Ocean University, Keelung 20224, TaiwanDepartment of Food Science, National Taiwan Ocean University, Keelung 20224, TaiwanThis study investigated the effects of shrimp chitosan with 95% degree of deacetylation (DD95) in combination with clinical antibiotics on the growth and urease production of <i>Helicobacter pylori</i>. The inhibitory effect of DD95 on the adherence of <i>H. pylori</i> to the human intestinal carcinoma cells (TSGH9201) was also investigated. Five strains of <i>H. pylori</i>, including three standard strains and two strains of clinical isolates were used as the test strains. The inhibitory effects of DD95 on growth and urease production of various strains of <i>H. pylori</i> increased with increasing DD95 concentration and decreasing pH values from pH 6.0 to pH 2.0. Urease activity of <i>H. pylori</i> at pH 2.0 in the presence of 4000 μg/mL of DD95 decreased by 37.86% to 46.53%. In the presence of 50 μg/mL antibiotics of amoxicillin, tetracycline, or metronidazole at pH 6.0 and pH 2.0, <i>H. pylori</i> counts were decreased by 1.51–3.19, and 1.47–2.82 Log CFU/mL, respectively. Following the addition of 4000 μg/mL DD95 into the 50 μg/mL antibiotic-containing culture medium with pH 6.0 and pH 2.0, overall <i>H. pylori</i> counts were strongly decreased by 3.67–7.61 and 6.61–6.70 Log CFU/mL, respectively. Further, DD95 could inhibit the adherence of <i>H. pylori</i> on TSGH 9201 cells, as evidenced by fluorescent microscopy and thus may potentially protect against <i>H. pylori</i> infection.https://www.mdpi.com/1660-3397/18/11/542chitosanantibacterial activity<i>Helicobacter pylori</i>urease activityTSGH9201 |
| spellingShingle | Shun-Hsien Chang Pei-Ling Hsieh Guo-Jane Tsai Chitosan Inhibits <i>Helicobacter pylori</i> Growth and Urease Production and Prevents Its Infection of Human Gastric Carcinoma Cells Marine Drugs chitosan antibacterial activity <i>Helicobacter pylori</i> urease activity TSGH9201 |
| title | Chitosan Inhibits <i>Helicobacter pylori</i> Growth and Urease Production and Prevents Its Infection of Human Gastric Carcinoma Cells |
| title_full | Chitosan Inhibits <i>Helicobacter pylori</i> Growth and Urease Production and Prevents Its Infection of Human Gastric Carcinoma Cells |
| title_fullStr | Chitosan Inhibits <i>Helicobacter pylori</i> Growth and Urease Production and Prevents Its Infection of Human Gastric Carcinoma Cells |
| title_full_unstemmed | Chitosan Inhibits <i>Helicobacter pylori</i> Growth and Urease Production and Prevents Its Infection of Human Gastric Carcinoma Cells |
| title_short | Chitosan Inhibits <i>Helicobacter pylori</i> Growth and Urease Production and Prevents Its Infection of Human Gastric Carcinoma Cells |
| title_sort | chitosan inhibits i helicobacter pylori i growth and urease production and prevents its infection of human gastric carcinoma cells |
| topic | chitosan antibacterial activity <i>Helicobacter pylori</i> urease activity TSGH9201 |
| url | https://www.mdpi.com/1660-3397/18/11/542 |
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