Effects of fish oil on glomerular function in rats with diabetes mellitus.
The mechanisms responsible for hyperfiltration in diabetes mellitus (DM) as well as for the initiation and progression of diabetic nephropathy are not fully elucidated. Enhanced prostaglandin E2 (PGE2) production has been invoked in the former and thromboxane (TXB2) and hyperlipidemia in the latter....
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
1990-07-01
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| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520426306 |
| _version_ | 1819058187623464960 |
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| author | AK Sinha LA Scharschmidt R Neuwirth H Holthofer N Gibbons CM Arbeeny D Schlondorff |
| author_facet | AK Sinha LA Scharschmidt R Neuwirth H Holthofer N Gibbons CM Arbeeny D Schlondorff |
| author_sort | AK Sinha |
| collection | DOAJ |
| description | The mechanisms responsible for hyperfiltration in diabetes mellitus (DM) as well as for the initiation and progression of diabetic nephropathy are not fully elucidated. Enhanced prostaglandin E2 (PGE2) production has been invoked in the former and thromboxane (TXB2) and hyperlipidemia in the latter. Fish oil (FO)-enriched diets can favorably alter eicosanoid synthesis and serum lipid profiles. We therefore examined the effects of a FO-enriched diet on glomerular filtration (GFR), proteinuria, glomerular eicosanoid production, and serum lipids in rats with streptozotocin-induced DM (STZ-DM). Groups of 5-8 rats with STZ-DM were maintained on low insulin and then pair-fed with isocaloric diets enriched with either FO (20% w/w) or beef tallow (BT; 20% w/w). GFR was determined in the same animals at onset of diet and after 8 and 20 weeks on the respective diets by [14C]inulin clearance using implanted osmotic minipumps each time. Significant hyperfiltration was present initially and GFR did not change on either diet for 20 weeks, in spite of a significant and greater than 50% decrease in all prostaglandins (PGE2, TXB2, PGF2 alpha, 6-keto, PGF1 alpha) produced by glomeruli isolated from DM/FO as compared to DM/BT or control rats. FO diet completely corrected the hypertriglyceridemia of diabetes and significantly reduced the mild and early proteinuria of DM. The decrease in proteinuria and the correction of hyperlipidemia of DM by a FO-enriched diet may be beneficial in the long term not only for the development of diabetic glomerulopathy, but also for the accelerated atherosclerosis of DM. |
| first_indexed | 2024-12-21T13:51:13Z |
| format | Article |
| id | doaj.art-3aec7db6d85d48f6a74e30835b4d9fcf |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-21T13:51:13Z |
| publishDate | 1990-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-3aec7db6d85d48f6a74e30835b4d9fcf2022-12-21T19:01:41ZengElsevierJournal of Lipid Research0022-22751990-07-0131712191228Effects of fish oil on glomerular function in rats with diabetes mellitus.AK Sinha0LA Scharschmidt1R Neuwirth2H Holthofer3N Gibbons4CM Arbeeny5D Schlondorff6Department of Medicine, Albert Einstein College of Medicine, Bronx, NY 10467.Department of Medicine, Albert Einstein College of Medicine, Bronx, NY 10467.Department of Medicine, Albert Einstein College of Medicine, Bronx, NY 10467.Department of Medicine, Albert Einstein College of Medicine, Bronx, NY 10467.Department of Medicine, Albert Einstein College of Medicine, Bronx, NY 10467.Department of Medicine, Albert Einstein College of Medicine, Bronx, NY 10467.Department of Medicine, Albert Einstein College of Medicine, Bronx, NY 10467.The mechanisms responsible for hyperfiltration in diabetes mellitus (DM) as well as for the initiation and progression of diabetic nephropathy are not fully elucidated. Enhanced prostaglandin E2 (PGE2) production has been invoked in the former and thromboxane (TXB2) and hyperlipidemia in the latter. Fish oil (FO)-enriched diets can favorably alter eicosanoid synthesis and serum lipid profiles. We therefore examined the effects of a FO-enriched diet on glomerular filtration (GFR), proteinuria, glomerular eicosanoid production, and serum lipids in rats with streptozotocin-induced DM (STZ-DM). Groups of 5-8 rats with STZ-DM were maintained on low insulin and then pair-fed with isocaloric diets enriched with either FO (20% w/w) or beef tallow (BT; 20% w/w). GFR was determined in the same animals at onset of diet and after 8 and 20 weeks on the respective diets by [14C]inulin clearance using implanted osmotic minipumps each time. Significant hyperfiltration was present initially and GFR did not change on either diet for 20 weeks, in spite of a significant and greater than 50% decrease in all prostaglandins (PGE2, TXB2, PGF2 alpha, 6-keto, PGF1 alpha) produced by glomeruli isolated from DM/FO as compared to DM/BT or control rats. FO diet completely corrected the hypertriglyceridemia of diabetes and significantly reduced the mild and early proteinuria of DM. The decrease in proteinuria and the correction of hyperlipidemia of DM by a FO-enriched diet may be beneficial in the long term not only for the development of diabetic glomerulopathy, but also for the accelerated atherosclerosis of DM.http://www.sciencedirect.com/science/article/pii/S0022227520426306 |
| spellingShingle | AK Sinha LA Scharschmidt R Neuwirth H Holthofer N Gibbons CM Arbeeny D Schlondorff Effects of fish oil on glomerular function in rats with diabetes mellitus. Journal of Lipid Research |
| title | Effects of fish oil on glomerular function in rats with diabetes mellitus. |
| title_full | Effects of fish oil on glomerular function in rats with diabetes mellitus. |
| title_fullStr | Effects of fish oil on glomerular function in rats with diabetes mellitus. |
| title_full_unstemmed | Effects of fish oil on glomerular function in rats with diabetes mellitus. |
| title_short | Effects of fish oil on glomerular function in rats with diabetes mellitus. |
| title_sort | effects of fish oil on glomerular function in rats with diabetes mellitus |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520426306 |
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