Alginate Hydrogels Coated with Chitosan for Wound Dressing
In this work, a coating of chitosan onto alginate hydrogels was realized using the water-soluble hydrochloride form of chitosan (CH-Cl), with the dual purpose of imparting antibacterial activity and delaying the release of hydrophilic molecules from the alginate matrix. Alginate hydrogels with diffe...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2015-05-01
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| Series: | Marine Drugs |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1660-3397/13/5/2890 |
| _version_ | 1798002608334438400 |
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| author | Maria Cristina Straccia Giovanna Gomez d'Ayala Ida Romano Adriana Oliva Paola Laurienzo |
| author_facet | Maria Cristina Straccia Giovanna Gomez d'Ayala Ida Romano Adriana Oliva Paola Laurienzo |
| author_sort | Maria Cristina Straccia |
| collection | DOAJ |
| description | In this work, a coating of chitosan onto alginate hydrogels was realized using the water-soluble hydrochloride form of chitosan (CH-Cl), with the dual purpose of imparting antibacterial activity and delaying the release of hydrophilic molecules from the alginate matrix. Alginate hydrogels with different calcium contents were prepared by the internal setting method and coated by immersion in a CH-Cl solution. Structural analysis by cryo-scanning electron microscopy was carried out to highlight morphological alterations due to the coating layer. Tests in vitro with human mesenchymal stromal cells (MSC) were assessed to check the absence of toxicity of CH-Cl. Swelling, stability in physiological solution and release characteristics using rhodamine B as the hydrophilic model drug were compared to those of relative uncoated hydrogels. Finally, antibacterial activity against Escherichia coli was tested. Results show that alginate hydrogels coated with chitosan hydrochloride described here can be proposed as a novel medicated dressing by associating intrinsic antimicrobial activity with improved sustained release characteristics. |
| first_indexed | 2024-04-11T11:55:02Z |
| format | Article |
| id | doaj.art-3af0b25902614cd2a9198343bec737de |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-11T11:55:02Z |
| publishDate | 2015-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-3af0b25902614cd2a9198343bec737de2022-12-22T04:25:11ZengMDPI AGMarine Drugs1660-33972015-05-011352890290810.3390/md13052890md13052890Alginate Hydrogels Coated with Chitosan for Wound DressingMaria Cristina Straccia0Giovanna Gomez d'Ayala1Ida Romano2Adriana Oliva3Paola Laurienzo4Institute for Polymers, Composites and Biomaterials (IPCB), CNR, via Campi Flegrei 34, Pozzuoli 80078, ItalyInstitute for Polymers, Composites and Biomaterials (IPCB), CNR, via Campi Flegrei 34, Pozzuoli 80078, ItalyInstitute of Biomolecular Chemistry, CNR, via Campi Flegrei 34, Pozzuoli 80078, ItalyDepartment of Biochemistry, Biophysics and General Pathology, Second University of Naples, via L. De Crecchio 7, Naples 80138, ItalyInstitute for Polymers, Composites and Biomaterials (IPCB), CNR, via Campi Flegrei 34, Pozzuoli 80078, ItalyIn this work, a coating of chitosan onto alginate hydrogels was realized using the water-soluble hydrochloride form of chitosan (CH-Cl), with the dual purpose of imparting antibacterial activity and delaying the release of hydrophilic molecules from the alginate matrix. Alginate hydrogels with different calcium contents were prepared by the internal setting method and coated by immersion in a CH-Cl solution. Structural analysis by cryo-scanning electron microscopy was carried out to highlight morphological alterations due to the coating layer. Tests in vitro with human mesenchymal stromal cells (MSC) were assessed to check the absence of toxicity of CH-Cl. Swelling, stability in physiological solution and release characteristics using rhodamine B as the hydrophilic model drug were compared to those of relative uncoated hydrogels. Finally, antibacterial activity against Escherichia coli was tested. Results show that alginate hydrogels coated with chitosan hydrochloride described here can be proposed as a novel medicated dressing by associating intrinsic antimicrobial activity with improved sustained release characteristics.http://www.mdpi.com/1660-3397/13/5/2890chitosan hydrochloridealginatehydrogelsantibacterial activitysustained releasewound dressing |
| spellingShingle | Maria Cristina Straccia Giovanna Gomez d'Ayala Ida Romano Adriana Oliva Paola Laurienzo Alginate Hydrogels Coated with Chitosan for Wound Dressing Marine Drugs chitosan hydrochloride alginate hydrogels antibacterial activity sustained release wound dressing |
| title | Alginate Hydrogels Coated with Chitosan for Wound Dressing |
| title_full | Alginate Hydrogels Coated with Chitosan for Wound Dressing |
| title_fullStr | Alginate Hydrogels Coated with Chitosan for Wound Dressing |
| title_full_unstemmed | Alginate Hydrogels Coated with Chitosan for Wound Dressing |
| title_short | Alginate Hydrogels Coated with Chitosan for Wound Dressing |
| title_sort | alginate hydrogels coated with chitosan for wound dressing |
| topic | chitosan hydrochloride alginate hydrogels antibacterial activity sustained release wound dressing |
| url | http://www.mdpi.com/1660-3397/13/5/2890 |
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