Effects of Acute Aerobic Exercise on Rats Serum Extracellular Vesicles Diameter, Concentration and Small RNAs Content
Physical exercise stimulates organs, mainly the skeletal muscle, to release a broad range of molecules, recently dubbed exerkines. Among them, RNAs, such as miRNAs, piRNAs, and tRNAs loaded in extracellular vesicles (EVs) have the potential to play a significant role in the way muscle and other orga...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-05-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphys.2018.00532/full |
| _version_ | 1819261776750968832 |
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| author | Getúlio P. Oliveira William F. Porto Cintia C. Palu Cintia C. Palu Lydyane M. Pereira Bernardo Petriz Bernardo Petriz Jeeser A. Almeida Juliane Viana Juliane Viana Nezio N. A. Filho Octavio L. Franco Octavio L. Franco Octavio L. Franco Octavio L. Franco Rinaldo W. Pereira Rinaldo W. Pereira Rinaldo W. Pereira |
| author_facet | Getúlio P. Oliveira William F. Porto Cintia C. Palu Cintia C. Palu Lydyane M. Pereira Bernardo Petriz Bernardo Petriz Jeeser A. Almeida Juliane Viana Juliane Viana Nezio N. A. Filho Octavio L. Franco Octavio L. Franco Octavio L. Franco Octavio L. Franco Rinaldo W. Pereira Rinaldo W. Pereira Rinaldo W. Pereira |
| author_sort | Getúlio P. Oliveira |
| collection | DOAJ |
| description | Physical exercise stimulates organs, mainly the skeletal muscle, to release a broad range of molecules, recently dubbed exerkines. Among them, RNAs, such as miRNAs, piRNAs, and tRNAs loaded in extracellular vesicles (EVs) have the potential to play a significant role in the way muscle and other organs communicate to translate exercise into health. Low, moderate and high intensity treadmill protocols were applied to rat groups, aiming to investigate the impact of exercise on serum EVs and their associated small RNA molecules. Transmission electron microscopy, resistive pulse sensing, and western blotting were used to investigate EVs morphology, size distribution, concentration and EVs marker proteins. Small RNA libraries from EVs RNA were sequenced. Exercise did not change EVs size, while increased EVs concentration. Twelve miRNAs were found differentially expressed after exercise: rno-miR-128-3p, 103-3p, 330-5p, 148a-3p, 191a-5p, 10b-5p, 93-5p, 25-3p, 142-5p, 3068-3p, 142-3p, and 410-3p. No piRNA was found differentially expressed, and one tRNA, trna8336, was found down-regulated after exercise. The differentially expressed miRNAs were predicted to target genes involved in the MAPK pathway. A single bout of exercise impacts EVs and their small RNA load, reinforcing the need for a more detailed investigation into EVs and their load as mediators of health-promoting exercise. |
| first_indexed | 2024-12-23T19:47:11Z |
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| id | doaj.art-3af63eb17d7341bba5388572d4a35a7b |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-23T19:47:11Z |
| publishDate | 2018-05-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Physiology |
| spelling | doaj.art-3af63eb17d7341bba5388572d4a35a7b2022-12-21T17:33:30ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2018-05-01910.3389/fphys.2018.00532348942Effects of Acute Aerobic Exercise on Rats Serum Extracellular Vesicles Diameter, Concentration and Small RNAs ContentGetúlio P. Oliveira0William F. Porto1Cintia C. Palu2Cintia C. Palu3Lydyane M. Pereira4Bernardo Petriz5Bernardo Petriz6Jeeser A. Almeida7Juliane Viana8Juliane Viana9Nezio N. A. Filho10Octavio L. Franco11Octavio L. Franco12Octavio L. Franco13Octavio L. Franco14Rinaldo W. Pereira15Rinaldo W. Pereira16Rinaldo W. Pereira17Programa de Pós-Graduação em Patologia Molecular, Universidade de Brasília, Brasília, BrazilS-Inova Biotech, Pós-Graduação em Biotecnologia, Universidade Católica Dom Bosco, Campo Grande, BrazilBioinformatics, NSilico Life Science Ltd., Cork, IrelandUniversity College Cork, Cork, IrelandPrograma de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília, BrazilPrograma de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília, BrazilCentro Universitário UDF, Brasília, BrazilPrograma de Pós-Graduação em Saúde e Desenvolvimento na Região Centro Oeste, Universidade Federal de Mato Grosso do Sul, Campo Grande, BrazilPrograma de Pós-Graduação em Patologia Molecular, Universidade de Brasília, Brasília, BrazilPrograma de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília, BrazilPrograma de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília, BrazilPrograma de Pós-Graduação em Patologia Molecular, Universidade de Brasília, Brasília, BrazilS-Inova Biotech, Pós-Graduação em Biotecnologia, Universidade Católica Dom Bosco, Campo Grande, BrazilPrograma de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília, BrazilPrograma de Pós-Graduação em Educação Física, Universidade Católica de Brasília, Brasília, BrazilPrograma de Pós-Graduação em Patologia Molecular, Universidade de Brasília, Brasília, BrazilPrograma de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília, BrazilPrograma de Pós-Graduação em Educação Física, Universidade Católica de Brasília, Brasília, BrazilPhysical exercise stimulates organs, mainly the skeletal muscle, to release a broad range of molecules, recently dubbed exerkines. Among them, RNAs, such as miRNAs, piRNAs, and tRNAs loaded in extracellular vesicles (EVs) have the potential to play a significant role in the way muscle and other organs communicate to translate exercise into health. Low, moderate and high intensity treadmill protocols were applied to rat groups, aiming to investigate the impact of exercise on serum EVs and their associated small RNA molecules. Transmission electron microscopy, resistive pulse sensing, and western blotting were used to investigate EVs morphology, size distribution, concentration and EVs marker proteins. Small RNA libraries from EVs RNA were sequenced. Exercise did not change EVs size, while increased EVs concentration. Twelve miRNAs were found differentially expressed after exercise: rno-miR-128-3p, 103-3p, 330-5p, 148a-3p, 191a-5p, 10b-5p, 93-5p, 25-3p, 142-5p, 3068-3p, 142-3p, and 410-3p. No piRNA was found differentially expressed, and one tRNA, trna8336, was found down-regulated after exercise. The differentially expressed miRNAs were predicted to target genes involved in the MAPK pathway. A single bout of exercise impacts EVs and their small RNA load, reinforcing the need for a more detailed investigation into EVs and their load as mediators of health-promoting exercise.https://www.frontiersin.org/article/10.3389/fphys.2018.00532/fullextracellular vesiclessmall RNA (smallRNA)aerobic exerciseNextGeneedgeR |
| spellingShingle | Getúlio P. Oliveira William F. Porto Cintia C. Palu Cintia C. Palu Lydyane M. Pereira Bernardo Petriz Bernardo Petriz Jeeser A. Almeida Juliane Viana Juliane Viana Nezio N. A. Filho Octavio L. Franco Octavio L. Franco Octavio L. Franco Octavio L. Franco Rinaldo W. Pereira Rinaldo W. Pereira Rinaldo W. Pereira Effects of Acute Aerobic Exercise on Rats Serum Extracellular Vesicles Diameter, Concentration and Small RNAs Content Frontiers in Physiology extracellular vesicles small RNA (smallRNA) aerobic exercise NextGene edgeR |
| title | Effects of Acute Aerobic Exercise on Rats Serum Extracellular Vesicles Diameter, Concentration and Small RNAs Content |
| title_full | Effects of Acute Aerobic Exercise on Rats Serum Extracellular Vesicles Diameter, Concentration and Small RNAs Content |
| title_fullStr | Effects of Acute Aerobic Exercise on Rats Serum Extracellular Vesicles Diameter, Concentration and Small RNAs Content |
| title_full_unstemmed | Effects of Acute Aerobic Exercise on Rats Serum Extracellular Vesicles Diameter, Concentration and Small RNAs Content |
| title_short | Effects of Acute Aerobic Exercise on Rats Serum Extracellular Vesicles Diameter, Concentration and Small RNAs Content |
| title_sort | effects of acute aerobic exercise on rats serum extracellular vesicles diameter concentration and small rnas content |
| topic | extracellular vesicles small RNA (smallRNA) aerobic exercise NextGene edgeR |
| url | https://www.frontiersin.org/article/10.3389/fphys.2018.00532/full |
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