MiR-219-5p inhibits growth and metastasis of ovarian cancer cells by targeting HMGA2
Abstract Background Accumulating studies have demonstrated that high-mobility group A2 (HMGA2), an oncofetal protein, plays a role in tumor development and progression. However, the molecular role of HMGA2 in ovarian carcinoma is yet to be established. MicroRNAs (miRNAs), a group of small noncoding...
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| Format: | Article |
| Language: | English |
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BMC
2018-11-01
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| Series: | Biological Research |
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| Online Access: | http://link.springer.com/article/10.1186/s40659-018-0199-y |
| _version_ | 1819155014568902656 |
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| author | Feng Xing Zhijiao Song Yuanying He |
| author_facet | Feng Xing Zhijiao Song Yuanying He |
| author_sort | Feng Xing |
| collection | DOAJ |
| description | Abstract Background Accumulating studies have demonstrated that high-mobility group A2 (HMGA2), an oncofetal protein, plays a role in tumor development and progression. However, the molecular role of HMGA2 in ovarian carcinoma is yet to be established. MicroRNAs (miRNAs), a group of small noncoding RNAs, negatively regulate gene expression and their dysregulation has been implicated in tumorigenesis. The aim of this study was to investigate the potential involvement of a specific miRNA, miR-219-5p, in HMGA2-induced ovarian cancer. Methods The ovarian cancer cell line, SKOV3, was employed, and miR-219-5p and HMGA2 overexpression vectors constructed. The CCK-8 kit was used to determine cell proliferation and the Transwell® assay used to measure cell invasion and migration. RT-PCR and western blot analyses were applied to analyze the expression of miR-219-5p and HMGA2, and the luciferase reporter assay used to examine the interactions between miR-219-5p and HMGA2. Nude mice were employed to characterize in vivo tumor growth regulation. Results Expression of miR-219-5p led to suppression of proliferation, invasion and migration of the ovarian cancer cell line, SKOV3, by targeting HMGA2. The inhibitory effects of miR-219-5p were reversed upon overexpression of HMGA2. Data from the luciferase reporter assay showed that miR-219-5p downregulates HMGA2 via direct integration with its 3′-UTR. Consistent with in vitro findings, expression of miR-219-5p led to significant inhibition of tumor growth in vivo. Conclusion Our results collectively suggest that miR-219-5p inhibits tumor growth and metastasis by targeting HMGA2. |
| first_indexed | 2024-12-22T15:30:14Z |
| format | Article |
| id | doaj.art-3af6593045b14bb3adac4130c1bd6dbf |
| institution | Directory Open Access Journal |
| issn | 0717-6287 |
| language | English |
| last_indexed | 2024-12-22T15:30:14Z |
| publishDate | 2018-11-01 |
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| record_format | Article |
| series | Biological Research |
| spelling | doaj.art-3af6593045b14bb3adac4130c1bd6dbf2022-12-21T18:21:23ZengBMCBiological Research0717-62872018-11-015111710.1186/s40659-018-0199-yMiR-219-5p inhibits growth and metastasis of ovarian cancer cells by targeting HMGA2Feng Xing0Zhijiao Song1Yuanying He2Department of Obstetrics and Gynecology, Shanghai Tenth People’s Hospital of Tongji University, Tongji University School of MedicineDepartment of Obstetrics and Gynecology, Shanghai Tenth People’s Hospital of Tongji University, Tongji University School of MedicineDepartment of Obstetrics and Gynecology, Shanghai Tenth People’s Hospital of Tongji University, Tongji University School of MedicineAbstract Background Accumulating studies have demonstrated that high-mobility group A2 (HMGA2), an oncofetal protein, plays a role in tumor development and progression. However, the molecular role of HMGA2 in ovarian carcinoma is yet to be established. MicroRNAs (miRNAs), a group of small noncoding RNAs, negatively regulate gene expression and their dysregulation has been implicated in tumorigenesis. The aim of this study was to investigate the potential involvement of a specific miRNA, miR-219-5p, in HMGA2-induced ovarian cancer. Methods The ovarian cancer cell line, SKOV3, was employed, and miR-219-5p and HMGA2 overexpression vectors constructed. The CCK-8 kit was used to determine cell proliferation and the Transwell® assay used to measure cell invasion and migration. RT-PCR and western blot analyses were applied to analyze the expression of miR-219-5p and HMGA2, and the luciferase reporter assay used to examine the interactions between miR-219-5p and HMGA2. Nude mice were employed to characterize in vivo tumor growth regulation. Results Expression of miR-219-5p led to suppression of proliferation, invasion and migration of the ovarian cancer cell line, SKOV3, by targeting HMGA2. The inhibitory effects of miR-219-5p were reversed upon overexpression of HMGA2. Data from the luciferase reporter assay showed that miR-219-5p downregulates HMGA2 via direct integration with its 3′-UTR. Consistent with in vitro findings, expression of miR-219-5p led to significant inhibition of tumor growth in vivo. Conclusion Our results collectively suggest that miR-219-5p inhibits tumor growth and metastasis by targeting HMGA2.http://link.springer.com/article/10.1186/s40659-018-0199-yOvarian cancermiR-219-5pHMGA2GrowthMetastasis |
| spellingShingle | Feng Xing Zhijiao Song Yuanying He MiR-219-5p inhibits growth and metastasis of ovarian cancer cells by targeting HMGA2 Biological Research Ovarian cancer miR-219-5p HMGA2 Growth Metastasis |
| title | MiR-219-5p inhibits growth and metastasis of ovarian cancer cells by targeting HMGA2 |
| title_full | MiR-219-5p inhibits growth and metastasis of ovarian cancer cells by targeting HMGA2 |
| title_fullStr | MiR-219-5p inhibits growth and metastasis of ovarian cancer cells by targeting HMGA2 |
| title_full_unstemmed | MiR-219-5p inhibits growth and metastasis of ovarian cancer cells by targeting HMGA2 |
| title_short | MiR-219-5p inhibits growth and metastasis of ovarian cancer cells by targeting HMGA2 |
| title_sort | mir 219 5p inhibits growth and metastasis of ovarian cancer cells by targeting hmga2 |
| topic | Ovarian cancer miR-219-5p HMGA2 Growth Metastasis |
| url | http://link.springer.com/article/10.1186/s40659-018-0199-y |
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