Delivery of platelet TPM3 mRNA into breast cancer cells via microvesicles enhances metastasis
Platelets are implicated in the pathophysiology of breast and other cancers through their role in exchanging biomolecules with tumor cells in the tumor microenvironment. Such exchange results in tumor‐educated platelets with altered RNA expression profiles. Multiple lines of evidence indicate that p...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019-12-01
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| Series: | FEBS Open Bio |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/2211-5463.12759 |
| _version_ | 1797542870474817536 |
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| author | Bing Yao Shuang Qu Ruifeng Hu Wen Gao Shidai Jin Junyi Ju Quan Zhao |
| author_facet | Bing Yao Shuang Qu Ruifeng Hu Wen Gao Shidai Jin Junyi Ju Quan Zhao |
| author_sort | Bing Yao |
| collection | DOAJ |
| description | Platelets are implicated in the pathophysiology of breast and other cancers through their role in exchanging biomolecules with tumor cells in the tumor microenvironment. Such exchange results in tumor‐educated platelets with altered RNA expression profiles. Multiple lines of evidence indicate that platelet RNA profiles may be suitable as diagnostic biomarkers for cancer‐related biological processes. In this study, we characterized the gene expression signatures of platelets in breast cancer (BC) by high‐throughput sequencing and quantitative real‐time RT‐PCR. Our results indicate that the expression of TPM3 (tropomyosin 3) mRNA is significantly elevated in platelets from patients with BC compared with age‐matched healthy control subjects. Furthermore, up‐regulation of TPM3 mRNA in platelets was found to be significantly correlated with metastasis in patients with BC. Finally, we report that platelet TPM3 mRNA is delivered into BC cells through microvesicles and leads to enhanced migrative phenotype of BC cells. In summary, our findings suggest that the transfer of platelet TPM3 mRNA into cancer cells via microvesicles promotes cancer cell migration, and thus platelet‐derived TPM3 mRNA may be a suitable biomarker for early diagnosis of metastatic BC. |
| first_indexed | 2024-03-10T13:37:33Z |
| format | Article |
| id | doaj.art-3af745915af542e780e9287aa73d0800 |
| institution | Directory Open Access Journal |
| issn | 2211-5463 |
| language | English |
| last_indexed | 2024-03-10T13:37:33Z |
| publishDate | 2019-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj.art-3af745915af542e780e9287aa73d08002023-11-21T07:01:44ZengWileyFEBS Open Bio2211-54632019-12-019122159216910.1002/2211-5463.12759Delivery of platelet TPM3 mRNA into breast cancer cells via microvesicles enhances metastasisBing Yao0Shuang Qu1Ruifeng Hu2Wen Gao3Shidai Jin4Junyi Ju5Quan Zhao6The State Key Laboratory of Pharmaceutical Biotechnology School of Life Sciences Nanjing University ChinaThe State Key Laboratory of Pharmaceutical Biotechnology School of Life Sciences Nanjing University ChinaThe State Key Laboratory of Pharmaceutical Biotechnology School of Life Sciences Nanjing University ChinaDepartment of Oncology The First Affiliated Hospital of Nanjing Medical University ChinaDepartment of Oncology The First Affiliated Hospital of Nanjing Medical University ChinaThe State Key Laboratory of Pharmaceutical Biotechnology School of Life Sciences Nanjing University ChinaThe State Key Laboratory of Pharmaceutical Biotechnology School of Life Sciences Nanjing University ChinaPlatelets are implicated in the pathophysiology of breast and other cancers through their role in exchanging biomolecules with tumor cells in the tumor microenvironment. Such exchange results in tumor‐educated platelets with altered RNA expression profiles. Multiple lines of evidence indicate that platelet RNA profiles may be suitable as diagnostic biomarkers for cancer‐related biological processes. In this study, we characterized the gene expression signatures of platelets in breast cancer (BC) by high‐throughput sequencing and quantitative real‐time RT‐PCR. Our results indicate that the expression of TPM3 (tropomyosin 3) mRNA is significantly elevated in platelets from patients with BC compared with age‐matched healthy control subjects. Furthermore, up‐regulation of TPM3 mRNA in platelets was found to be significantly correlated with metastasis in patients with BC. Finally, we report that platelet TPM3 mRNA is delivered into BC cells through microvesicles and leads to enhanced migrative phenotype of BC cells. In summary, our findings suggest that the transfer of platelet TPM3 mRNA into cancer cells via microvesicles promotes cancer cell migration, and thus platelet‐derived TPM3 mRNA may be a suitable biomarker for early diagnosis of metastatic BC.https://doi.org/10.1002/2211-5463.12759biomarkerbreast cancerplateletRNATPM3 |
| spellingShingle | Bing Yao Shuang Qu Ruifeng Hu Wen Gao Shidai Jin Junyi Ju Quan Zhao Delivery of platelet TPM3 mRNA into breast cancer cells via microvesicles enhances metastasis FEBS Open Bio biomarker breast cancer platelet RNA TPM3 |
| title | Delivery of platelet TPM3 mRNA into breast cancer cells via microvesicles enhances metastasis |
| title_full | Delivery of platelet TPM3 mRNA into breast cancer cells via microvesicles enhances metastasis |
| title_fullStr | Delivery of platelet TPM3 mRNA into breast cancer cells via microvesicles enhances metastasis |
| title_full_unstemmed | Delivery of platelet TPM3 mRNA into breast cancer cells via microvesicles enhances metastasis |
| title_short | Delivery of platelet TPM3 mRNA into breast cancer cells via microvesicles enhances metastasis |
| title_sort | delivery of platelet tpm3 mrna into breast cancer cells via microvesicles enhances metastasis |
| topic | biomarker breast cancer platelet RNA TPM3 |
| url | https://doi.org/10.1002/2211-5463.12759 |
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