Oral microbiome contributes to the failure of orthodontic temporary anchorage devices (TADs)
Abstract Background The stability of temporary anchorage devices (TADs) is critical in orthodontic clinics. The failure of TADs is multifactorial, and the role of the oral microbiome has not been clearly defined. Herein, we attempted to analyze the contribution of the oral microbiome to the failure...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-01-01
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| Series: | BMC Oral Health |
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| Online Access: | https://doi.org/10.1186/s12903-023-02715-7 |
| _version_ | 1828056631692230656 |
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| author | Ningrui Zhao Qian Zhang Yanning Guo Shengjie Cui Yajing Tian Yidan Zhang Yanheng Zhou Xuedong Wang |
| author_facet | Ningrui Zhao Qian Zhang Yanning Guo Shengjie Cui Yajing Tian Yidan Zhang Yanheng Zhou Xuedong Wang |
| author_sort | Ningrui Zhao |
| collection | DOAJ |
| description | Abstract Background The stability of temporary anchorage devices (TADs) is critical in orthodontic clinics. The failure of TADs is multifactorial, and the role of the oral microbiome has not been clearly defined. Herein, we attempted to analyze the contribution of the oral microbiome to the failure of TADs. Methods Next-generation sequencing was adopted for analyzing the microbiome on the TADs from orthodontic patients. 29 TADs (15 failed TADs and 14 successful TADs) were used for 16S rRNA gene sequencing. A total of 135 TADs (62 failed TADs and 73 successful TADs) were collected to conduct metagenomic sequencing. Additionally, 34 verified samples (18 failed TADs and 16 successful TADs) were collected for quantitative real-time polymerase chain reaction analysis (qRT-PCR). Results Successful and failed TADs demonstrated discrepancies in microbiome structure, composition, and function. Clear separations were found in β-diversity in 16S rRNA gene sequencing as well as metagenomic sequencing (p < 0.05). Metagenomic sequencing showed that Prevotella intermedia , Eikenella corrodens, Parvimonas spp., Neisseria elongata, and Catonella morbi were enriched in the failed groups. qRT-PCR also demonstrated that the absolute bacteria load of Prevotella intermedia was higher in failed TADs (p < 0.05). Considering functional aspects, the failed group showed enriched genes involved in flagellar assembly, bacterial chemotaxis, and oxidative phosphorylation. Conclusions This study illustrated the compositional and functional differences of microorganisms found on successful and failed TADs, indicating that controlling bacterial adhesion on the surface of TADs is essential for their success rate. |
| first_indexed | 2024-04-10T20:58:39Z |
| format | Article |
| id | doaj.art-3b0268fa14e544778fd1c15ab1325e4f |
| institution | Directory Open Access Journal |
| issn | 1472-6831 |
| language | English |
| last_indexed | 2024-04-10T20:58:39Z |
| publishDate | 2023-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Oral Health |
| spelling | doaj.art-3b0268fa14e544778fd1c15ab1325e4f2023-01-22T12:27:07ZengBMCBMC Oral Health1472-68312023-01-0123111410.1186/s12903-023-02715-7Oral microbiome contributes to the failure of orthodontic temporary anchorage devices (TADs)Ningrui Zhao0Qian Zhang1Yanning Guo2Shengjie Cui3Yajing Tian4Yidan Zhang5Yanheng Zhou6Xuedong Wang7Department of Orthodontics, Peking University School and Hospital of Stomatology & National Center of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology & Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health & NMPA Key Laboratory for Dental MaterialsCentral Laboratory, Peking University School and Hospital of StomatologyDepartment of Orthodontics, Peking University School and Hospital of Stomatology & National Center of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology & Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health & NMPA Key Laboratory for Dental MaterialsDepartment of Orthodontics, Peking University School and Hospital of Stomatology & National Center of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology & Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health & NMPA Key Laboratory for Dental MaterialsDepartment of Orthodontics, Peking University School and Hospital of Stomatology & National Center of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology & Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health & NMPA Key Laboratory for Dental MaterialsDepartment of Orthodontics, Peking University School and Hospital of Stomatology & National Center of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology & Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health & NMPA Key Laboratory for Dental MaterialsDepartment of Orthodontics, Peking University School and Hospital of Stomatology & National Center of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology & Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health & NMPA Key Laboratory for Dental MaterialsDepartment of Orthodontics, Peking University School and Hospital of Stomatology & National Center of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology & Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health & NMPA Key Laboratory for Dental MaterialsAbstract Background The stability of temporary anchorage devices (TADs) is critical in orthodontic clinics. The failure of TADs is multifactorial, and the role of the oral microbiome has not been clearly defined. Herein, we attempted to analyze the contribution of the oral microbiome to the failure of TADs. Methods Next-generation sequencing was adopted for analyzing the microbiome on the TADs from orthodontic patients. 29 TADs (15 failed TADs and 14 successful TADs) were used for 16S rRNA gene sequencing. A total of 135 TADs (62 failed TADs and 73 successful TADs) were collected to conduct metagenomic sequencing. Additionally, 34 verified samples (18 failed TADs and 16 successful TADs) were collected for quantitative real-time polymerase chain reaction analysis (qRT-PCR). Results Successful and failed TADs demonstrated discrepancies in microbiome structure, composition, and function. Clear separations were found in β-diversity in 16S rRNA gene sequencing as well as metagenomic sequencing (p < 0.05). Metagenomic sequencing showed that Prevotella intermedia , Eikenella corrodens, Parvimonas spp., Neisseria elongata, and Catonella morbi were enriched in the failed groups. qRT-PCR also demonstrated that the absolute bacteria load of Prevotella intermedia was higher in failed TADs (p < 0.05). Considering functional aspects, the failed group showed enriched genes involved in flagellar assembly, bacterial chemotaxis, and oxidative phosphorylation. Conclusions This study illustrated the compositional and functional differences of microorganisms found on successful and failed TADs, indicating that controlling bacterial adhesion on the surface of TADs is essential for their success rate.https://doi.org/10.1186/s12903-023-02715-7MicrobiologyOrthodonticsTemporary anchorage devices (TADs)Next-generation sequencing |
| spellingShingle | Ningrui Zhao Qian Zhang Yanning Guo Shengjie Cui Yajing Tian Yidan Zhang Yanheng Zhou Xuedong Wang Oral microbiome contributes to the failure of orthodontic temporary anchorage devices (TADs) BMC Oral Health Microbiology Orthodontics Temporary anchorage devices (TADs) Next-generation sequencing |
| title | Oral microbiome contributes to the failure of orthodontic temporary anchorage devices (TADs) |
| title_full | Oral microbiome contributes to the failure of orthodontic temporary anchorage devices (TADs) |
| title_fullStr | Oral microbiome contributes to the failure of orthodontic temporary anchorage devices (TADs) |
| title_full_unstemmed | Oral microbiome contributes to the failure of orthodontic temporary anchorage devices (TADs) |
| title_short | Oral microbiome contributes to the failure of orthodontic temporary anchorage devices (TADs) |
| title_sort | oral microbiome contributes to the failure of orthodontic temporary anchorage devices tads |
| topic | Microbiology Orthodontics Temporary anchorage devices (TADs) Next-generation sequencing |
| url | https://doi.org/10.1186/s12903-023-02715-7 |
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