Effect of one-time high load exercise on skeletal muscle injury in rats of different genders: oxidative stress and mitochondrial responses
ABSTRACT Purpose: To evaluate the impact of one-time high load exercise on skeletal muscle injury and analysis its mechanism in different genders. Methods: Twenty-four male and 24 female rats were divided randomly into four groups respectively: control, 0 h, 6 h, and 24 h after exercise. The activ...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2022-12-01
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| Series: | Acta Cirúrgica Brasileira |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502022000800204&tlng=en |
| _version_ | 1811199315848200192 |
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| author | Yuan Wang Mengmeng Chen Yan Gao Kang He Zhaoyun Yang Yuewei Li Shuang Zhang Lijing Zhao |
| author_facet | Yuan Wang Mengmeng Chen Yan Gao Kang He Zhaoyun Yang Yuewei Li Shuang Zhang Lijing Zhao |
| author_sort | Yuan Wang |
| collection | DOAJ |
| description | ABSTRACT Purpose: To evaluate the impact of one-time high load exercise on skeletal muscle injury and analysis its mechanism in different genders. Methods: Twenty-four male and 24 female rats were divided randomly into four groups respectively: control, 0 h, 6 h, and 24 h after exercise. The activities of creatine kinase (CK), lactate dehydrogenase (LDH), and myohemoglobin (MYO) in serum, the expression level of oxidative stress markers, mitochondrial respiratory chain complex enzyme, and the apoptosis related protein in quadriceps were detected. Results: The results showed that the activities of CK, LDH and MYO in serum increased immediately after exercise and restored faster in female rats. More obvious structural disorder and apoptosis in male rats were showed. Malondialdehyde (MDA) and superoxide dismutase (SOD) were increased while catalase (CAT) and glutathione (GSH) were decreased in male rats. SOD, CAT and GSH were increased in female rats. Mitochondrial complex enzyme activity was decreased in males and increased in females. Conclusions: The skeletal muscle injury in both genders of rat could be induced by one-time high load exercise due to the mitochondrial respiratory enzyme dysfunction and oxidative stress, which was relatively mild and recovered quicker in female rats. |
| first_indexed | 2024-04-12T01:45:51Z |
| format | Article |
| id | doaj.art-3b0448aab6344bb4ba9ea10e31ee9d09 |
| institution | Directory Open Access Journal |
| issn | 1678-2674 |
| language | English |
| last_indexed | 2024-04-12T01:45:51Z |
| publishDate | 2022-12-01 |
| publisher | Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
| record_format | Article |
| series | Acta Cirúrgica Brasileira |
| spelling | doaj.art-3b0448aab6344bb4ba9ea10e31ee9d092022-12-22T03:53:05ZengSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaActa Cirúrgica Brasileira1678-26742022-12-0137810.1590/acb370805Effect of one-time high load exercise on skeletal muscle injury in rats of different genders: oxidative stress and mitochondrial responsesYuan Wanghttps://orcid.org/0000-0003-2881-5191Mengmeng Chenhttps://orcid.org/0000-0003-4179-0427Yan Gaohttps://orcid.org/0000-0002-6541-8577Kang Hehttps://orcid.org/0000-0001-9621-9258Zhaoyun Yanghttps://orcid.org/0000-0003-3037-8824Yuewei Lihttps://orcid.org/0000-0001-9178-643XShuang Zhanghttps://orcid.org/0000-0003-4927-7230Lijing Zhaohttps://orcid.org/0000-0002-3190-7399ABSTRACT Purpose: To evaluate the impact of one-time high load exercise on skeletal muscle injury and analysis its mechanism in different genders. Methods: Twenty-four male and 24 female rats were divided randomly into four groups respectively: control, 0 h, 6 h, and 24 h after exercise. The activities of creatine kinase (CK), lactate dehydrogenase (LDH), and myohemoglobin (MYO) in serum, the expression level of oxidative stress markers, mitochondrial respiratory chain complex enzyme, and the apoptosis related protein in quadriceps were detected. Results: The results showed that the activities of CK, LDH and MYO in serum increased immediately after exercise and restored faster in female rats. More obvious structural disorder and apoptosis in male rats were showed. Malondialdehyde (MDA) and superoxide dismutase (SOD) were increased while catalase (CAT) and glutathione (GSH) were decreased in male rats. SOD, CAT and GSH were increased in female rats. Mitochondrial complex enzyme activity was decreased in males and increased in females. Conclusions: The skeletal muscle injury in both genders of rat could be induced by one-time high load exercise due to the mitochondrial respiratory enzyme dysfunction and oxidative stress, which was relatively mild and recovered quicker in female rats.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502022000800204&tlng=enExerciseMuscleSkeletalInjuryGender |
| spellingShingle | Yuan Wang Mengmeng Chen Yan Gao Kang He Zhaoyun Yang Yuewei Li Shuang Zhang Lijing Zhao Effect of one-time high load exercise on skeletal muscle injury in rats of different genders: oxidative stress and mitochondrial responses Acta Cirúrgica Brasileira Exercise Muscle Skeletal Injury Gender |
| title | Effect of one-time high load exercise on skeletal muscle injury in rats of different genders: oxidative stress and mitochondrial responses |
| title_full | Effect of one-time high load exercise on skeletal muscle injury in rats of different genders: oxidative stress and mitochondrial responses |
| title_fullStr | Effect of one-time high load exercise on skeletal muscle injury in rats of different genders: oxidative stress and mitochondrial responses |
| title_full_unstemmed | Effect of one-time high load exercise on skeletal muscle injury in rats of different genders: oxidative stress and mitochondrial responses |
| title_short | Effect of one-time high load exercise on skeletal muscle injury in rats of different genders: oxidative stress and mitochondrial responses |
| title_sort | effect of one time high load exercise on skeletal muscle injury in rats of different genders oxidative stress and mitochondrial responses |
| topic | Exercise Muscle Skeletal Injury Gender |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502022000800204&tlng=en |
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