Salt-mediated changes in leaf mesophyll cells of Lycopersicon esculentum Mill. plants
Five-week-old tomato plants (Lycopersicon esculentum) cv. Perkoz grown in pots containing garden soil in a growth chamber were submitted to 50 or 150 mM NaCl for 1 h, 2 and 5 days. Tomato leaf anatomy generally did not change after short time salinity, except 5-day-treatment with 150 mM NaCl, where...
| Main Authors: | , |
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| Format: | Article |
| Language: | Bulgarian |
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University of Zagreb, Faculty of Agriculture
2014-09-01
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| Series: | Journal of Central European Agriculture |
| Subjects: | |
| Online Access: | http://jcea.agr.hr/articles/774329_Salt_mediated_changes_in_leaf_mesophyll_cells_of_Lycopersicon_esculentum_Mill_plants_en.pdf |
| _version_ | 1819208172578013184 |
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| author | Magdalena Gapinska Slawa Glinska |
| author_facet | Magdalena Gapinska Slawa Glinska |
| author_sort | Magdalena Gapinska |
| collection | DOAJ |
| description | Five-week-old tomato plants (Lycopersicon esculentum) cv. Perkoz grown in pots containing garden soil in a growth chamber were submitted to 50 or 150 mM NaCl for 1 h, 2 and 5 days. Tomato leaf anatomy generally did not change after short time salinity, except 5-day-treatment with 150 mM NaCl, where changed cell shape (shrunk and deformed) simultaneously with increased volume of intercellular spaces (IS) were observed. Although leaf hydration (H) depleted only 1 h after 150 mM NaCl treatment both salt concentrations generated two coexisting populations of salt-affected mesophyll cells: (i) slightly-affected (Sl-A) which showed incipient plasmolysis or slightly changed shapes, and (ii) severely-affected (Sv-A) which showed severe plasmolysis; serious deformation of cell shape or disorganization including cell degeneration. In Sl-A cells salinity changed location and shape of chloroplasts which were: more rounded, with oversized starch grains (SG) (2d) or more flat (5d). Salt-mediated changes were becoming more distinguished and pronounced with length of 150 mM NaCl treatment. The amount of salt-affected cells was changing during the experiment and depended on the salt concentration. In 50 mM-treated plants salt-affected cells appeared 1 h after treatment (~40%) and raised up to 78% on 2nd day, however the population of Sl-A cells dominated. In 150 mM NaCl-treated plants the percentage of affected cells raised during the experiment from 75% to 99%. Firstly Sl-A cells dominated, but on the 5th day the majority was Sv-A. Salt-affected cells were distributed quite evenly in palisade or spongy mesophyll, except 2 d after treatment with 50 mM NaCl, when their number was higher in the palisade mesophyll. Sv-A cells in the spongy mesophyll were located mostly near the bundle while in the palisade mesophyll more irregularly. Different susceptibility of cells to salt stress might be the consequence of an unequal distribution of osmotic stress and subsequent ionic stress or physiological state of cells. |
| first_indexed | 2024-12-23T05:35:10Z |
| format | Article |
| id | doaj.art-3b05d24de49740ada15724d7251a2359 |
| institution | Directory Open Access Journal |
| issn | 1332-9049 |
| language | Bulgarian |
| last_indexed | 2024-12-23T05:35:10Z |
| publishDate | 2014-09-01 |
| publisher | University of Zagreb, Faculty of Agriculture |
| record_format | Article |
| series | Journal of Central European Agriculture |
| spelling | doaj.art-3b05d24de49740ada15724d7251a23592022-12-21T17:58:21ZbulUniversity of Zagreb, Faculty of AgricultureJournal of Central European Agriculture1332-90492014-09-0115321923510.5513/JCEA01/15.3.1478Salt-mediated changes in leaf mesophyll cells of Lycopersicon esculentum Mill. plantsMagdalena Gapinska Slawa Glinska Five-week-old tomato plants (Lycopersicon esculentum) cv. Perkoz grown in pots containing garden soil in a growth chamber were submitted to 50 or 150 mM NaCl for 1 h, 2 and 5 days. Tomato leaf anatomy generally did not change after short time salinity, except 5-day-treatment with 150 mM NaCl, where changed cell shape (shrunk and deformed) simultaneously with increased volume of intercellular spaces (IS) were observed. Although leaf hydration (H) depleted only 1 h after 150 mM NaCl treatment both salt concentrations generated two coexisting populations of salt-affected mesophyll cells: (i) slightly-affected (Sl-A) which showed incipient plasmolysis or slightly changed shapes, and (ii) severely-affected (Sv-A) which showed severe plasmolysis; serious deformation of cell shape or disorganization including cell degeneration. In Sl-A cells salinity changed location and shape of chloroplasts which were: more rounded, with oversized starch grains (SG) (2d) or more flat (5d). Salt-mediated changes were becoming more distinguished and pronounced with length of 150 mM NaCl treatment. The amount of salt-affected cells was changing during the experiment and depended on the salt concentration. In 50 mM-treated plants salt-affected cells appeared 1 h after treatment (~40%) and raised up to 78% on 2nd day, however the population of Sl-A cells dominated. In 150 mM NaCl-treated plants the percentage of affected cells raised during the experiment from 75% to 99%. Firstly Sl-A cells dominated, but on the 5th day the majority was Sv-A. Salt-affected cells were distributed quite evenly in palisade or spongy mesophyll, except 2 d after treatment with 50 mM NaCl, when their number was higher in the palisade mesophyll. Sv-A cells in the spongy mesophyll were located mostly near the bundle while in the palisade mesophyll more irregularly. Different susceptibility of cells to salt stress might be the consequence of an unequal distribution of osmotic stress and subsequent ionic stress or physiological state of cells.http://jcea.agr.hr/articles/774329_Salt_mediated_changes_in_leaf_mesophyll_cells_of_Lycopersicon_esculentum_Mill_plants_en.pdfleaf hydrationmesophyll cellsosmotic stressplasmolysissalt stresstomato |
| spellingShingle | Magdalena Gapinska Slawa Glinska Salt-mediated changes in leaf mesophyll cells of Lycopersicon esculentum Mill. plants Journal of Central European Agriculture leaf hydration mesophyll cells osmotic stress plasmolysis salt stress tomato |
| title | Salt-mediated changes in leaf mesophyll cells of Lycopersicon esculentum Mill. plants |
| title_full | Salt-mediated changes in leaf mesophyll cells of Lycopersicon esculentum Mill. plants |
| title_fullStr | Salt-mediated changes in leaf mesophyll cells of Lycopersicon esculentum Mill. plants |
| title_full_unstemmed | Salt-mediated changes in leaf mesophyll cells of Lycopersicon esculentum Mill. plants |
| title_short | Salt-mediated changes in leaf mesophyll cells of Lycopersicon esculentum Mill. plants |
| title_sort | salt mediated changes in leaf mesophyll cells of lycopersicon esculentum mill plants |
| topic | leaf hydration mesophyll cells osmotic stress plasmolysis salt stress tomato |
| url | http://jcea.agr.hr/articles/774329_Salt_mediated_changes_in_leaf_mesophyll_cells_of_Lycopersicon_esculentum_Mill_plants_en.pdf |
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