Assessment of centrifugation using for accelerated immunological microarray analysis for blood cells investigation
Phase of incubation microarray with cell suspension is prolonged when cells are investigated. It takes from 20 to 60 min if cell sedimentation on the surface of microarray is the result of gravity . Decrease of this stage duration is possible due to centrifugation. In th is article influence of cent...
| Main Authors: | , , |
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| Format: | Article |
| Language: | Russian |
| Published: |
ABV-press
2014-07-01
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| Series: | Онкогематология |
| Subjects: | |
| Online Access: | https://oncohematology.abvpress.ru/ongm/article/view/93 |
| _version_ | 1797855531135664128 |
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| author | A. V. Shishkin N. G. Ovchinina S. S. Bessmeltsev |
| author_facet | A. V. Shishkin N. G. Ovchinina S. S. Bessmeltsev |
| author_sort | A. V. Shishkin |
| collection | DOAJ |
| description | Phase of incubation microarray with cell suspension is prolonged when cells are investigated. It takes from 20 to 60 min if cell sedimentation on the surface of microarray is the result of gravity . Decrease of this stage duration is possible due to centrifugation. In th is article influence of centrifugation on results of analysis is considered. Changes of morphological description of cells are estimated when they a re precipitatedwith different acceleration. Also availability of centrifugation using when it is necessary to obtain the high density of cell binding in test regions of microarray if cells concentration in sample is small is demonstrated. |
| first_indexed | 2024-04-09T20:25:15Z |
| format | Article |
| id | doaj.art-3b062479faf045d1a8380e70fdc57c18 |
| institution | Directory Open Access Journal |
| issn | 1818-8346 2413-4023 |
| language | Russian |
| last_indexed | 2024-04-09T20:25:15Z |
| publishDate | 2014-07-01 |
| publisher | ABV-press |
| record_format | Article |
| series | Онкогематология |
| spelling | doaj.art-3b062479faf045d1a8380e70fdc57c182023-03-30T20:15:10ZrusABV-pressОнкогематология1818-83462413-40232014-07-0163768110.17650/1818-8346-2011-6-3-76-81108Assessment of centrifugation using for accelerated immunological microarray analysis for blood cells investigationA. V. Shishkin0N. G. Ovchinina1S. S. Bessmeltsev2Izhevsk State Medical AcademyIzhevsk State Medical AcademyRussian Research Institute of Hematology and TransfusiologyPhase of incubation microarray with cell suspension is prolonged when cells are investigated. It takes from 20 to 60 min if cell sedimentation on the surface of microarray is the result of gravity . Decrease of this stage duration is possible due to centrifugation. In th is article influence of centrifugation on results of analysis is considered. Changes of morphological description of cells are estimated when they a re precipitatedwith different acceleration. Also availability of centrifugation using when it is necessary to obtain the high density of cell binding in test regions of microarray if cells concentration in sample is small is demonstrated.https://oncohematology.abvpress.ru/ongm/article/view/93immunological microarraysurface cell antigens detectioncell sedimentation |
| spellingShingle | A. V. Shishkin N. G. Ovchinina S. S. Bessmeltsev Assessment of centrifugation using for accelerated immunological microarray analysis for blood cells investigation Онкогематология immunological microarray surface cell antigens detection cell sedimentation |
| title | Assessment of centrifugation using for accelerated immunological microarray analysis for blood cells investigation |
| title_full | Assessment of centrifugation using for accelerated immunological microarray analysis for blood cells investigation |
| title_fullStr | Assessment of centrifugation using for accelerated immunological microarray analysis for blood cells investigation |
| title_full_unstemmed | Assessment of centrifugation using for accelerated immunological microarray analysis for blood cells investigation |
| title_short | Assessment of centrifugation using for accelerated immunological microarray analysis for blood cells investigation |
| title_sort | assessment of centrifugation using for accelerated immunological microarray analysis for blood cells investigation |
| topic | immunological microarray surface cell antigens detection cell sedimentation |
| url | https://oncohematology.abvpress.ru/ongm/article/view/93 |
| work_keys_str_mv | AT avshishkin assessmentofcentrifugationusingforacceleratedimmunologicalmicroarrayanalysisforbloodcellsinvestigation AT ngovchinina assessmentofcentrifugationusingforacceleratedimmunologicalmicroarrayanalysisforbloodcellsinvestigation AT ssbessmeltsev assessmentofcentrifugationusingforacceleratedimmunologicalmicroarrayanalysisforbloodcellsinvestigation |