Orostachys japonicus ethyl acetate fraction suppresses MRSA biofilm formation
Objective: To investigate the effect of Orostachys (O.) japonicus, a perennial herbaceous plant of the Family Crassulaceae, on biofilm formed by methicillin-resistant Staphylococcus aureus (MRSA). Methods: Powdered O. japonicus was extracted by 95% methanol, concentrated, and then, systematically fr...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2020-01-01
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| Series: | Asian Pacific Journal of Tropical Medicine |
| Subjects: | |
| Online Access: | http://www.apjtm.org/article.asp?issn=1995-7645;year=2020;volume=13;issue=1;spage=38;epage=45;aulast=Kim |
| _version_ | 1811266996292026368 |
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| author | Jae-Hyeon Kim Su-Yeon Han Ji-Hye Kwon Dong-Seok Lee |
| author_facet | Jae-Hyeon Kim Su-Yeon Han Ji-Hye Kwon Dong-Seok Lee |
| author_sort | Jae-Hyeon Kim |
| collection | DOAJ |
| description | Objective: To investigate the effect of Orostachys (O.) japonicus, a perennial herbaceous plant of the Family Crassulaceae, on biofilm formed by methicillin-resistant Staphylococcus aureus (MRSA).
Methods: Powdered O. japonicus was extracted by 95% methanol, concentrated, and then, systematically fractionated with n-hexane, dichloromethane (DCM), ethyl acetate (EtOAc), n-butanol, and H2O according to polarity. Among them, the flavonoid-rich EtOAc fraction demonstrated the highest antibacterial activity and was used in this study. Using the biofilm inhibition assay, cell-surface attachment assay, confocal laser scanning microscopy, latex agglutination assay, and real time qRT-PCR, we examined whether the EtOAc fraction inhibited the formation of MRSA biofilm.
Results: The EtOAc fraction exhibited distinct activity against biofilm formation and cell-surface attachment of MRSA up to 1 mg/mL through down-regulating the expression of mecA gene and the production and agglutination of penicillin-binding protein 2a as solidly observed in biofilm inhibition assay, cell-suface attachment assay, confocal laser scanning microscopy, latex agglutination assay, and real time qRT-PCR analysis.
Conclusions: These results suggest that O. japonicus could be utilized as a potential resource for the development of new anti-biofilm formation of MRSA and antibacterial agents in the future. |
| first_indexed | 2024-04-12T20:53:23Z |
| format | Article |
| id | doaj.art-3b07d41bf0fd47e8b2a65cb0305aefac |
| institution | Directory Open Access Journal |
| issn | 2352-4146 |
| language | English |
| last_indexed | 2024-04-12T20:53:23Z |
| publishDate | 2020-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Tropical Medicine |
| spelling | doaj.art-3b07d41bf0fd47e8b2a65cb0305aefac2022-12-22T03:17:03ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Medicine2352-41462020-01-01131384510.4103/1995-7645.273573Orostachys japonicus ethyl acetate fraction suppresses MRSA biofilm formationJae-Hyeon KimSu-Yeon HanJi-Hye KwonDong-Seok LeeObjective: To investigate the effect of Orostachys (O.) japonicus, a perennial herbaceous plant of the Family Crassulaceae, on biofilm formed by methicillin-resistant Staphylococcus aureus (MRSA). Methods: Powdered O. japonicus was extracted by 95% methanol, concentrated, and then, systematically fractionated with n-hexane, dichloromethane (DCM), ethyl acetate (EtOAc), n-butanol, and H2O according to polarity. Among them, the flavonoid-rich EtOAc fraction demonstrated the highest antibacterial activity and was used in this study. Using the biofilm inhibition assay, cell-surface attachment assay, confocal laser scanning microscopy, latex agglutination assay, and real time qRT-PCR, we examined whether the EtOAc fraction inhibited the formation of MRSA biofilm. Results: The EtOAc fraction exhibited distinct activity against biofilm formation and cell-surface attachment of MRSA up to 1 mg/mL through down-regulating the expression of mecA gene and the production and agglutination of penicillin-binding protein 2a as solidly observed in biofilm inhibition assay, cell-suface attachment assay, confocal laser scanning microscopy, latex agglutination assay, and real time qRT-PCR analysis. Conclusions: These results suggest that O. japonicus could be utilized as a potential resource for the development of new anti-biofilm formation of MRSA and antibacterial agents in the future.http://www.apjtm.org/article.asp?issn=1995-7645;year=2020;volume=13;issue=1;spage=38;epage=45;aulast=Kimorostachys japonicus; mrsa; biofilm formation |
| spellingShingle | Jae-Hyeon Kim Su-Yeon Han Ji-Hye Kwon Dong-Seok Lee Orostachys japonicus ethyl acetate fraction suppresses MRSA biofilm formation Asian Pacific Journal of Tropical Medicine orostachys japonicus; mrsa; biofilm formation |
| title | Orostachys japonicus ethyl acetate fraction suppresses MRSA biofilm formation |
| title_full | Orostachys japonicus ethyl acetate fraction suppresses MRSA biofilm formation |
| title_fullStr | Orostachys japonicus ethyl acetate fraction suppresses MRSA biofilm formation |
| title_full_unstemmed | Orostachys japonicus ethyl acetate fraction suppresses MRSA biofilm formation |
| title_short | Orostachys japonicus ethyl acetate fraction suppresses MRSA biofilm formation |
| title_sort | orostachys japonicus ethyl acetate fraction suppresses mrsa biofilm formation |
| topic | orostachys japonicus; mrsa; biofilm formation |
| url | http://www.apjtm.org/article.asp?issn=1995-7645;year=2020;volume=13;issue=1;spage=38;epage=45;aulast=Kim |
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