Physiological traits and metabolic profiles of contrasting rice cultivars under mild salinity stress during the seedling stage
This study aimed to investigate the effects of mild salinity stress on some physiological traits and metabolic profiles of rice during the seedling stage. Three rice cultivars differing in salinity stress response, ‘KDML105’ (salt-sensitive, local Thai jasmine rice), ‘IR29’ (salt-sensitive rice), a...
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| Format: | Article |
| Language: | English |
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AcademicPres
2023-06-01
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| Series: | Notulae Botanicae Horti Agrobotanici Cluj-Napoca |
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| Online Access: | https://www.notulaebotanicae.ro/index.php/nbha/article/view/13211 |
| _version_ | 1827912514927591424 |
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| author | Thitiwan JUMPA Jutarop PHETCHARABURANIN Manida SUKSAWAT Wattana PATTANAGUL |
| author_facet | Thitiwan JUMPA Jutarop PHETCHARABURANIN Manida SUKSAWAT Wattana PATTANAGUL |
| author_sort | Thitiwan JUMPA |
| collection | DOAJ |
| description |
This study aimed to investigate the effects of mild salinity stress on some physiological traits and metabolic profiles of rice during the seedling stage. Three rice cultivars differing in salinity stress response, ‘KDML105’ (salt-sensitive, local Thai jasmine rice), ‘IR29’ (salt-sensitive rice), and ‘Pokkali’ (salt-tolerant rice), were subjected to mild salinity stress by flood-irrigated with 40 mM NaCl. Salinity stress significantly reduces the growth and biomass of the sensitive cultivars. Electrolyte leakage in ‘KDML105’ and ‘IR29’ also increased significantly while the relative water content (RWC) decreased. However, salinity stress did not affect growth, electrolyte leakage, and RWC in the tolerant cultivar. Salinity significantly increased Na+/K+ ratio in shoot and root of all cultivars. Nevertheless, the increase of Na+/K+ ratio in ‘KDML105’ and ‘IR29’ was higher than that of ‘Pokkali’. The metabolic profile analysis of ‘KDML105’ showed that uracil, L-proline, malonate, L-carnitine, L-lactate, and guanine were significantly increased, but the levels of dihydroxyacetone and glycine were significantly decreased under salinity conditions. In ‘IR29’, L-proline, melatonin, gamma-aminobutyrate, and acetate were significantly increased. In ‘Pokkali’, however, sugar alcohols and sugars were slightly increased when exposed to salinity conditions. The metabolic pathway analysis revealed one involved metabolic pathway of ‘KDML105’: aminoacyl-tRNA biosynthesis, and three involved those of ‘IR29’: arginine proline metabolism, sulfur metabolism, and butanoate metabolism. The difference in the metabolic profile of the salt-tolerance salt-sensitive cultivars provides new insights to identify additional novel biomarkers that will help better understand the mechanisms for salt tolerance in rice.
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| id | doaj.art-3b0801e33d0f4468a4e1d208b157235b |
| institution | Directory Open Access Journal |
| issn | 0255-965X 1842-4309 |
| language | English |
| last_indexed | 2024-03-13T02:17:51Z |
| publishDate | 2023-06-01 |
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| series | Notulae Botanicae Horti Agrobotanici Cluj-Napoca |
| spelling | doaj.art-3b0801e33d0f4468a4e1d208b157235b2023-06-30T11:03:47ZengAcademicPresNotulae Botanicae Horti Agrobotanici Cluj-Napoca0255-965X1842-43092023-06-0151210.15835/nbha51213211Physiological traits and metabolic profiles of contrasting rice cultivars under mild salinity stress during the seedling stage Thitiwan JUMPA0Jutarop PHETCHARABURANIN1Manida SUKSAWAT2Wattana PATTANAGUL3Khon Kaen University, Faculty of Sciences, Department of Biology, 123 Moo 16 Mittapap Rd., Nai-Muang, Muang District, 40002, Khon KaenKhon Kaen University, Faculty of Medicine, Department of Systems Biosciences and Computational Medicine, 123 Moo 16 Mittapap Rd., Nai-Muang, Muang District, 40002, Khon KaenKhon Kaen University, Faculty of Medicine, Department of Systems Biosciences and Computational Medicine, 123 Moo 16 Mittapap Rd., Nai-Muang, Muang District, 40002, Khon KaenKhon Kaen University, Faculty of Sciences, Department of Biology, 123 Moo 16 Mittapap Rd., Nai-Muang, Muang District, 40002, Khon Kaen This study aimed to investigate the effects of mild salinity stress on some physiological traits and metabolic profiles of rice during the seedling stage. Three rice cultivars differing in salinity stress response, ‘KDML105’ (salt-sensitive, local Thai jasmine rice), ‘IR29’ (salt-sensitive rice), and ‘Pokkali’ (salt-tolerant rice), were subjected to mild salinity stress by flood-irrigated with 40 mM NaCl. Salinity stress significantly reduces the growth and biomass of the sensitive cultivars. Electrolyte leakage in ‘KDML105’ and ‘IR29’ also increased significantly while the relative water content (RWC) decreased. However, salinity stress did not affect growth, electrolyte leakage, and RWC in the tolerant cultivar. Salinity significantly increased Na+/K+ ratio in shoot and root of all cultivars. Nevertheless, the increase of Na+/K+ ratio in ‘KDML105’ and ‘IR29’ was higher than that of ‘Pokkali’. The metabolic profile analysis of ‘KDML105’ showed that uracil, L-proline, malonate, L-carnitine, L-lactate, and guanine were significantly increased, but the levels of dihydroxyacetone and glycine were significantly decreased under salinity conditions. In ‘IR29’, L-proline, melatonin, gamma-aminobutyrate, and acetate were significantly increased. In ‘Pokkali’, however, sugar alcohols and sugars were slightly increased when exposed to salinity conditions. The metabolic pathway analysis revealed one involved metabolic pathway of ‘KDML105’: aminoacyl-tRNA biosynthesis, and three involved those of ‘IR29’: arginine proline metabolism, sulfur metabolism, and butanoate metabolism. The difference in the metabolic profile of the salt-tolerance salt-sensitive cultivars provides new insights to identify additional novel biomarkers that will help better understand the mechanisms for salt tolerance in rice. https://www.notulaebotanicae.ro/index.php/nbha/article/view/132111H-NMRmetabolic profilesphysiological traitsricesalinity stress |
| spellingShingle | Thitiwan JUMPA Jutarop PHETCHARABURANIN Manida SUKSAWAT Wattana PATTANAGUL Physiological traits and metabolic profiles of contrasting rice cultivars under mild salinity stress during the seedling stage Notulae Botanicae Horti Agrobotanici Cluj-Napoca 1H-NMR metabolic profiles physiological traits rice salinity stress |
| title | Physiological traits and metabolic profiles of contrasting rice cultivars under mild salinity stress during the seedling stage |
| title_full | Physiological traits and metabolic profiles of contrasting rice cultivars under mild salinity stress during the seedling stage |
| title_fullStr | Physiological traits and metabolic profiles of contrasting rice cultivars under mild salinity stress during the seedling stage |
| title_full_unstemmed | Physiological traits and metabolic profiles of contrasting rice cultivars under mild salinity stress during the seedling stage |
| title_short | Physiological traits and metabolic profiles of contrasting rice cultivars under mild salinity stress during the seedling stage |
| title_sort | physiological traits and metabolic profiles of contrasting rice cultivars under mild salinity stress during the seedling stage |
| topic | 1H-NMR metabolic profiles physiological traits rice salinity stress |
| url | https://www.notulaebotanicae.ro/index.php/nbha/article/view/13211 |
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