Stability and Degradation of Caffeoylquinic Acids under Different Storage Conditions Studied by High-Performance Liquid Chromatography with Photo Diode Array Detection and High-Performance Liquid Chromatography with Electrospray Ionization Collision-Induced Dissociation Tandem Mass Spectrometry
Caffeoylquinic acids (CQAs) are main constituents in many herbal medicines with various biological and pharmacological effects. However, CQAs will degrade or isomerize when affected by temperature, pH, light, etc. In this study, high-performance liquid chromatography with photodiode array detection...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2016-07-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1420-3049/21/7/948 |
| _version_ | 1818297172224901120 |
|---|---|
| author | Meng Xue Hang Shi Jiao Zhang Qing-Quan Liu Jun Guan Jia-Yu Zhang Qun Ma |
| author_facet | Meng Xue Hang Shi Jiao Zhang Qing-Quan Liu Jun Guan Jia-Yu Zhang Qun Ma |
| author_sort | Meng Xue |
| collection | DOAJ |
| description | Caffeoylquinic acids (CQAs) are main constituents in many herbal medicines with various biological and pharmacological effects. However, CQAs will degrade or isomerize when affected by temperature, pH, light, etc. In this study, high-performance liquid chromatography with photodiode array detection (HPLC-PDA) and high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) was utilized to study the stability and degradation of CQAs (three mono-acyl CQAs and four di-acyl CQAs) under various ordinary storage conditions (involving different temperatures, solvents, and light irradiation). The results indicated that the stability of CQAs was mainly affected by temperature and light irradiation, while solvents did not affect it in any obvious way under the conditions studied. Mono-acyl CQAs were generally much more stable than di-acyl CQAs under the same conditions. Meanwhile, the chemical structures of 30 degradation products were also characterized by HPLC-MSn, inferring that isomerization, methylation, and hydrolysis were three major degradation pathways. The result provides a meaningful clue for the storage conditions of CQAs standard substances and samples. |
| first_indexed | 2024-12-13T04:15:12Z |
| format | Article |
| id | doaj.art-3b0ae1ba06e4412196befe41d3188573 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-13T04:15:12Z |
| publishDate | 2016-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-3b0ae1ba06e4412196befe41d31885732022-12-21T23:59:55ZengMDPI AGMolecules1420-30492016-07-0121794810.3390/molecules21070948molecules21070948Stability and Degradation of Caffeoylquinic Acids under Different Storage Conditions Studied by High-Performance Liquid Chromatography with Photo Diode Array Detection and High-Performance Liquid Chromatography with Electrospray Ionization Collision-Induced Dissociation Tandem Mass SpectrometryMeng Xue0Hang Shi1Jiao Zhang2Qing-Quan Liu3Jun Guan4Jia-Yu Zhang5Qun Ma6School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, ChinaSchool of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, ChinaSchool of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, ChinaBeijing Hospital of Traditional Chinese Medicine, Beijing 100010, ChinaSchool of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, ChinaBeijing Institution of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, ChinaSchool of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, ChinaCaffeoylquinic acids (CQAs) are main constituents in many herbal medicines with various biological and pharmacological effects. However, CQAs will degrade or isomerize when affected by temperature, pH, light, etc. In this study, high-performance liquid chromatography with photodiode array detection (HPLC-PDA) and high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) was utilized to study the stability and degradation of CQAs (three mono-acyl CQAs and four di-acyl CQAs) under various ordinary storage conditions (involving different temperatures, solvents, and light irradiation). The results indicated that the stability of CQAs was mainly affected by temperature and light irradiation, while solvents did not affect it in any obvious way under the conditions studied. Mono-acyl CQAs were generally much more stable than di-acyl CQAs under the same conditions. Meanwhile, the chemical structures of 30 degradation products were also characterized by HPLC-MSn, inferring that isomerization, methylation, and hydrolysis were three major degradation pathways. The result provides a meaningful clue for the storage conditions of CQAs standard substances and samples.http://www.mdpi.com/1420-3049/21/7/948caffeoylquinic acids (CQAs)stabilitytemperaturelight irradiationsolventHPLC-MSn |
| spellingShingle | Meng Xue Hang Shi Jiao Zhang Qing-Quan Liu Jun Guan Jia-Yu Zhang Qun Ma Stability and Degradation of Caffeoylquinic Acids under Different Storage Conditions Studied by High-Performance Liquid Chromatography with Photo Diode Array Detection and High-Performance Liquid Chromatography with Electrospray Ionization Collision-Induced Dissociation Tandem Mass Spectrometry Molecules caffeoylquinic acids (CQAs) stability temperature light irradiation solvent HPLC-MSn |
| title | Stability and Degradation of Caffeoylquinic Acids under Different Storage Conditions Studied by High-Performance Liquid Chromatography with Photo Diode Array Detection and High-Performance Liquid Chromatography with Electrospray Ionization Collision-Induced Dissociation Tandem Mass Spectrometry |
| title_full | Stability and Degradation of Caffeoylquinic Acids under Different Storage Conditions Studied by High-Performance Liquid Chromatography with Photo Diode Array Detection and High-Performance Liquid Chromatography with Electrospray Ionization Collision-Induced Dissociation Tandem Mass Spectrometry |
| title_fullStr | Stability and Degradation of Caffeoylquinic Acids under Different Storage Conditions Studied by High-Performance Liquid Chromatography with Photo Diode Array Detection and High-Performance Liquid Chromatography with Electrospray Ionization Collision-Induced Dissociation Tandem Mass Spectrometry |
| title_full_unstemmed | Stability and Degradation of Caffeoylquinic Acids under Different Storage Conditions Studied by High-Performance Liquid Chromatography with Photo Diode Array Detection and High-Performance Liquid Chromatography with Electrospray Ionization Collision-Induced Dissociation Tandem Mass Spectrometry |
| title_short | Stability and Degradation of Caffeoylquinic Acids under Different Storage Conditions Studied by High-Performance Liquid Chromatography with Photo Diode Array Detection and High-Performance Liquid Chromatography with Electrospray Ionization Collision-Induced Dissociation Tandem Mass Spectrometry |
| title_sort | stability and degradation of caffeoylquinic acids under different storage conditions studied by high performance liquid chromatography with photo diode array detection and high performance liquid chromatography with electrospray ionization collision induced dissociation tandem mass spectrometry |
| topic | caffeoylquinic acids (CQAs) stability temperature light irradiation solvent HPLC-MSn |
| url | http://www.mdpi.com/1420-3049/21/7/948 |
| work_keys_str_mv | AT mengxue stabilityanddegradationofcaffeoylquinicacidsunderdifferentstorageconditionsstudiedbyhighperformanceliquidchromatographywithphotodiodearraydetectionandhighperformanceliquidchromatographywithelectrosprayionizationcollisioninduceddissociationtandemmassspectr AT hangshi stabilityanddegradationofcaffeoylquinicacidsunderdifferentstorageconditionsstudiedbyhighperformanceliquidchromatographywithphotodiodearraydetectionandhighperformanceliquidchromatographywithelectrosprayionizationcollisioninduceddissociationtandemmassspectr AT jiaozhang stabilityanddegradationofcaffeoylquinicacidsunderdifferentstorageconditionsstudiedbyhighperformanceliquidchromatographywithphotodiodearraydetectionandhighperformanceliquidchromatographywithelectrosprayionizationcollisioninduceddissociationtandemmassspectr AT qingquanliu stabilityanddegradationofcaffeoylquinicacidsunderdifferentstorageconditionsstudiedbyhighperformanceliquidchromatographywithphotodiodearraydetectionandhighperformanceliquidchromatographywithelectrosprayionizationcollisioninduceddissociationtandemmassspectr AT junguan stabilityanddegradationofcaffeoylquinicacidsunderdifferentstorageconditionsstudiedbyhighperformanceliquidchromatographywithphotodiodearraydetectionandhighperformanceliquidchromatographywithelectrosprayionizationcollisioninduceddissociationtandemmassspectr AT jiayuzhang stabilityanddegradationofcaffeoylquinicacidsunderdifferentstorageconditionsstudiedbyhighperformanceliquidchromatographywithphotodiodearraydetectionandhighperformanceliquidchromatographywithelectrosprayionizationcollisioninduceddissociationtandemmassspectr AT qunma stabilityanddegradationofcaffeoylquinicacidsunderdifferentstorageconditionsstudiedbyhighperformanceliquidchromatographywithphotodiodearraydetectionandhighperformanceliquidchromatographywithelectrosprayionizationcollisioninduceddissociationtandemmassspectr |