Investigation of the Genotoxicological Profile of Aqueous <i>Betula pendula</i> Extracts
<i>Betula pendula</i> belongs to the <i>Betulaceae</i> family and is most common in the northern hemisphere. Various birch species have exhibited antimicrobial, antioxidant and anticancer properties. In the present study, we investigated the genotoxic and cytotoxic activity a...
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MDPI AG
2022-10-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/20/2673 |
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| author | Ioanna Efthimiou Dimitris Vlastos Vassilios Triantafyllidis Antonios Eleftherianos Maria Antonopoulou |
| author_facet | Ioanna Efthimiou Dimitris Vlastos Vassilios Triantafyllidis Antonios Eleftherianos Maria Antonopoulou |
| author_sort | Ioanna Efthimiou |
| collection | DOAJ |
| description | <i>Betula pendula</i> belongs to the <i>Betulaceae</i> family and is most common in the northern hemisphere. Various birch species have exhibited antimicrobial, antioxidant and anticancer properties. In the present study, we investigated the genotoxic and cytotoxic activity as well as the antigenotoxic potential against the mutagenic agent mitomycin-C (MMC) of two commercial products, i.e., a <i>Betula pendula</i> aqueous leaf extract product (BE) and a <i>Betula pendula</i> product containing aqueous extract of birch leaves at a percentage of 94% and lemon juice at a percentage of 6% (BP) using the cytokinesis block micronucleus (CBMN) assay. The most prevalent compounds and elements of BE and BP were identified using UHPLC-MS and ICP-MS/MS, respectively. All mixtures of BE with MMC demonstrated a decrease in the MN frequencies, with the lowest and highest concentrations inducing a statistically significant antigenotoxic activity. BP lacked genotoxic potential, while it was cytotoxic in all concentrations. Its mixtures with MMC demonstrated statistically significant antigenotoxic activity only at the lowest concentration. UHPLC-MS and ICP-MS/MS showed the presence of various elements and phytochemicals. Our results reveal antigenotoxic and cytotoxic potential of both BE and BP, while the variations observed could indicate the importance of the interactions among different natural products and/or their compounds. |
| first_indexed | 2024-03-09T19:33:28Z |
| format | Article |
| id | doaj.art-3b0d3e8dd65f4b03a0e76a7894b37779 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T19:33:28Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-3b0d3e8dd65f4b03a0e76a7894b377792023-11-24T02:03:14ZengMDPI AGPlants2223-77472022-10-011120267310.3390/plants11202673Investigation of the Genotoxicological Profile of Aqueous <i>Betula pendula</i> ExtractsIoanna Efthimiou0Dimitris Vlastos1Vassilios Triantafyllidis2Antonios Eleftherianos3Maria Antonopoulou4Department of Sustainable Agriculture, University of Patras, GR-30100 Agrinio, GreeceDepartment of Biology, Section of Genetics Cell Biology and Development, University of Patras, GR-26500 Patras, GreeceDepartment of Food Science & Technology, University of Patras, GR-30100 Agrinio, GreeceAkrokeramos Sewerage Laboratory, Athens Water Supply and Sewerage Company (EYDAP SA), GR-18755 Keratsini, GreeceDepartment of Sustainable Agriculture, University of Patras, GR-30100 Agrinio, Greece<i>Betula pendula</i> belongs to the <i>Betulaceae</i> family and is most common in the northern hemisphere. Various birch species have exhibited antimicrobial, antioxidant and anticancer properties. In the present study, we investigated the genotoxic and cytotoxic activity as well as the antigenotoxic potential against the mutagenic agent mitomycin-C (MMC) of two commercial products, i.e., a <i>Betula pendula</i> aqueous leaf extract product (BE) and a <i>Betula pendula</i> product containing aqueous extract of birch leaves at a percentage of 94% and lemon juice at a percentage of 6% (BP) using the cytokinesis block micronucleus (CBMN) assay. The most prevalent compounds and elements of BE and BP were identified using UHPLC-MS and ICP-MS/MS, respectively. All mixtures of BE with MMC demonstrated a decrease in the MN frequencies, with the lowest and highest concentrations inducing a statistically significant antigenotoxic activity. BP lacked genotoxic potential, while it was cytotoxic in all concentrations. Its mixtures with MMC demonstrated statistically significant antigenotoxic activity only at the lowest concentration. UHPLC-MS and ICP-MS/MS showed the presence of various elements and phytochemicals. Our results reveal antigenotoxic and cytotoxic potential of both BE and BP, while the variations observed could indicate the importance of the interactions among different natural products and/or their compounds.https://www.mdpi.com/2223-7747/11/20/2673<i>Betula pendula</i>cytotoxicitygenotoxicityantigenotoxicityhuman lymphocytescytokinesis block micronucleus assay |
| spellingShingle | Ioanna Efthimiou Dimitris Vlastos Vassilios Triantafyllidis Antonios Eleftherianos Maria Antonopoulou Investigation of the Genotoxicological Profile of Aqueous <i>Betula pendula</i> Extracts Plants <i>Betula pendula</i> cytotoxicity genotoxicity antigenotoxicity human lymphocytes cytokinesis block micronucleus assay |
| title | Investigation of the Genotoxicological Profile of Aqueous <i>Betula pendula</i> Extracts |
| title_full | Investigation of the Genotoxicological Profile of Aqueous <i>Betula pendula</i> Extracts |
| title_fullStr | Investigation of the Genotoxicological Profile of Aqueous <i>Betula pendula</i> Extracts |
| title_full_unstemmed | Investigation of the Genotoxicological Profile of Aqueous <i>Betula pendula</i> Extracts |
| title_short | Investigation of the Genotoxicological Profile of Aqueous <i>Betula pendula</i> Extracts |
| title_sort | investigation of the genotoxicological profile of aqueous i betula pendula i extracts |
| topic | <i>Betula pendula</i> cytotoxicity genotoxicity antigenotoxicity human lymphocytes cytokinesis block micronucleus assay |
| url | https://www.mdpi.com/2223-7747/11/20/2673 |
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