Next-generation genetic sexing strain establishment in the agricultural pest Ceratitis capitata
Abstract Tephritid fruit fly pests pose an increasing threat to the agricultural industry due to their global dispersion and a highly invasive nature. Here we showcase the feasibility of an early-detection SEPARATOR sex sorting approach through using the non-model Tephritid pest, Ceratitis capitata....
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-47276-5 |
| _version_ | 1827723367542685696 |
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| author | Serafima Davydova Junru Liu Nikolay P. Kandul W. Evan Braswell Omar S. Akbari Angela Meccariello |
| author_facet | Serafima Davydova Junru Liu Nikolay P. Kandul W. Evan Braswell Omar S. Akbari Angela Meccariello |
| author_sort | Serafima Davydova |
| collection | DOAJ |
| description | Abstract Tephritid fruit fly pests pose an increasing threat to the agricultural industry due to their global dispersion and a highly invasive nature. Here we showcase the feasibility of an early-detection SEPARATOR sex sorting approach through using the non-model Tephritid pest, Ceratitis capitata. This system relies on female-only fluorescent marker expression, accomplished through the use of a sex-specific intron of the highly-conserved transformer gene from C. capitata and Anastrepha ludens. The herein characterized strains have 100% desired phenotype outcomes, allowing accurate male–female separation during early development. Overall, we describe an antibiotic and temperature-independent sex-sorting system in C. capitata, which, moving forward, may be implemented in other non-model Tephritid pest species. This strategy can facilitate the establishment of genetic sexing systems with endogenous elements exclusively, which, on a wider scale, can improve pest population control strategies like sterile insect technique. |
| first_indexed | 2024-03-10T22:00:16Z |
| format | Article |
| id | doaj.art-3b0e3f82d1ac4a55b28f3198a93de13c |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-10T22:00:16Z |
| publishDate | 2023-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-3b0e3f82d1ac4a55b28f3198a93de13c2023-11-19T12:59:01ZengNature PortfolioScientific Reports2045-23222023-11-011311810.1038/s41598-023-47276-5Next-generation genetic sexing strain establishment in the agricultural pest Ceratitis capitataSerafima Davydova0Junru Liu1Nikolay P. Kandul2W. Evan Braswell3Omar S. Akbari4Angela Meccariello5Department of Life Sciences, Imperial College LondonDepartment of Cell and Developmental Biology, School of Biological Sciences, University of California, San DiegoDepartment of Cell and Developmental Biology, School of Biological Sciences, University of California, San DiegoUSDA APHIS PPQ Science and Technology Insect Management and Molecular Diagnostic LaboratoryDepartment of Cell and Developmental Biology, School of Biological Sciences, University of California, San DiegoDepartment of Life Sciences, Imperial College LondonAbstract Tephritid fruit fly pests pose an increasing threat to the agricultural industry due to their global dispersion and a highly invasive nature. Here we showcase the feasibility of an early-detection SEPARATOR sex sorting approach through using the non-model Tephritid pest, Ceratitis capitata. This system relies on female-only fluorescent marker expression, accomplished through the use of a sex-specific intron of the highly-conserved transformer gene from C. capitata and Anastrepha ludens. The herein characterized strains have 100% desired phenotype outcomes, allowing accurate male–female separation during early development. Overall, we describe an antibiotic and temperature-independent sex-sorting system in C. capitata, which, moving forward, may be implemented in other non-model Tephritid pest species. This strategy can facilitate the establishment of genetic sexing systems with endogenous elements exclusively, which, on a wider scale, can improve pest population control strategies like sterile insect technique.https://doi.org/10.1038/s41598-023-47276-5 |
| spellingShingle | Serafima Davydova Junru Liu Nikolay P. Kandul W. Evan Braswell Omar S. Akbari Angela Meccariello Next-generation genetic sexing strain establishment in the agricultural pest Ceratitis capitata Scientific Reports |
| title | Next-generation genetic sexing strain establishment in the agricultural pest Ceratitis capitata |
| title_full | Next-generation genetic sexing strain establishment in the agricultural pest Ceratitis capitata |
| title_fullStr | Next-generation genetic sexing strain establishment in the agricultural pest Ceratitis capitata |
| title_full_unstemmed | Next-generation genetic sexing strain establishment in the agricultural pest Ceratitis capitata |
| title_short | Next-generation genetic sexing strain establishment in the agricultural pest Ceratitis capitata |
| title_sort | next generation genetic sexing strain establishment in the agricultural pest ceratitis capitata |
| url | https://doi.org/10.1038/s41598-023-47276-5 |
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