RNA localization in cytoplasm and nucleus of human embryonic stem cells
Objective To investigate the localization of RNA in cytoplasm and nucleus of human embryonic stem cells (hESCs). Methods After separating cytoplasm and nucleus of hESCs, RNAs were extracted for sequencing, and the localization characteristics of RNAs were analyzed by bioinformatics. RT-qPCR was used...
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| Format: | Article |
| Language: | zho |
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2020-06-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a200233.pdf |
| _version_ | 1797365707402379264 |
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| author | ZHOU Fan-qi, FENG Wen-xin, TAN Pu-wen, MA Yan-ni, WANG Dong, YU Jia |
| author_facet | ZHOU Fan-qi, FENG Wen-xin, TAN Pu-wen, MA Yan-ni, WANG Dong, YU Jia |
| author_sort | ZHOU Fan-qi, FENG Wen-xin, TAN Pu-wen, MA Yan-ni, WANG Dong, YU Jia |
| collection | DOAJ |
| description | Objective To investigate the localization of RNA in cytoplasm and nucleus of human embryonic stem cells (hESCs). Methods After separating cytoplasm and nucleus of hESCs, RNAs were extracted for sequencing, and the localization characteristics of RNAs were analyzed by bioinformatics. RT-qPCR was used to verify the localization of these RNAs. Results More RNA types were found in the cytoplasm than nucleus, as well as specifically locational RNAs (2 952 in the cytoplasm and 634 in the nucleus, respectively). Conclusions The localization map of RNA in cytoplasm and nucleus of hESCs is described,and the localization of many RNAs is verified. |
| first_indexed | 2024-03-08T16:52:47Z |
| format | Article |
| id | doaj.art-3b0f22d2ad7b44aca6ad5b2e4acb529b |
| institution | Directory Open Access Journal |
| issn | 1001-6325 |
| language | zho |
| last_indexed | 2024-03-08T16:52:47Z |
| publishDate | 2020-06-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj.art-3b0f22d2ad7b44aca6ad5b2e4acb529b2024-01-05T03:15:51ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252020-06-01406765770RNA localization in cytoplasm and nucleus of human embryonic stem cellsZHOU Fan-qi, FENG Wen-xin, TAN Pu-wen, MA Yan-ni, WANG Dong, YU Jia01. State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences CAMS, School of Basic Medicine PUMC, Beijing 100005;;2. Henan Geology Vocational College, Zhengzhou 450000;;3. Department of Bioinformatics, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510000, ChinaObjective To investigate the localization of RNA in cytoplasm and nucleus of human embryonic stem cells (hESCs). Methods After separating cytoplasm and nucleus of hESCs, RNAs were extracted for sequencing, and the localization characteristics of RNAs were analyzed by bioinformatics. RT-qPCR was used to verify the localization of these RNAs. Results More RNA types were found in the cytoplasm than nucleus, as well as specifically locational RNAs (2 952 in the cytoplasm and 634 in the nucleus, respectively). Conclusions The localization map of RNA in cytoplasm and nucleus of hESCs is described,and the localization of many RNAs is verified.http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a200233.pdfhuman embryonic stem cells (hescs)|rna localization |
| spellingShingle | ZHOU Fan-qi, FENG Wen-xin, TAN Pu-wen, MA Yan-ni, WANG Dong, YU Jia RNA localization in cytoplasm and nucleus of human embryonic stem cells Jichu yixue yu linchuang human embryonic stem cells (hescs)|rna localization |
| title | RNA localization in cytoplasm and nucleus of human embryonic stem cells |
| title_full | RNA localization in cytoplasm and nucleus of human embryonic stem cells |
| title_fullStr | RNA localization in cytoplasm and nucleus of human embryonic stem cells |
| title_full_unstemmed | RNA localization in cytoplasm and nucleus of human embryonic stem cells |
| title_short | RNA localization in cytoplasm and nucleus of human embryonic stem cells |
| title_sort | rna localization in cytoplasm and nucleus of human embryonic stem cells |
| topic | human embryonic stem cells (hescs)|rna localization |
| url | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a200233.pdf |
| work_keys_str_mv | AT zhoufanqifengwenxintanpuwenmayanniwangdongyujia rnalocalizationincytoplasmandnucleusofhumanembryonicstemcells |