Bacillus spp. enzymatic activity to support circular economy
Agricultural and industrial waste represents the most abundant renewable resource in the biosphere and is readily available in substantial quantities. Recently, there has been a growing focus on harnessing the potential of these types of waste as raw materials for producing value-added p...
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| Format: | Article |
| Language: | English |
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Faculty of Technology, Novi Sad
2023-01-01
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| Series: | Acta Periodica Technologica |
| Subjects: | |
| Online Access: | https://doiserbia.nb.rs/img/doi/1450-7188/2023/1450-71882354325D.pdf |
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| author | Dujković Tatjana D. Pajčin Ivana S. Vlajkov Vanja R. Grahovac Jovana A. |
| author_facet | Dujković Tatjana D. Pajčin Ivana S. Vlajkov Vanja R. Grahovac Jovana A. |
| author_sort | Dujković Tatjana D. |
| collection | DOAJ |
| description | Agricultural and industrial waste represents the most abundant renewable
resource in the biosphere and is readily available in substantial
quantities. Recently, there has been a growing focus on harnessing the
potential of these types of waste as raw materials for producing value-added
products, such as microbial enzymes, using solid-state or submerged
fermentation systems. Among all the bacterial genera utilized in the
production of industrially important enzymes, the Bacillus genus stands out
as the most extensively employed for this purpose. In this study, the
production of various enzymes such as protease, pectinase, cellulase,
xylanase, lipase, and gelatinase was evaluated using the different Bacillus
strains isolated from vegetables’ rhizosphere. The growth zones and halos
were observed to determine production of the following enzymes: cellulases,
proteases, pectinases, xylanases, gelatinases and lipases, confirming the
breakdown of complex substrates in the medium. Bacillus Pap 3 exhibited the
highest EAI (enzymatic activity index) value (4.00) for pectinase activity,
indicating its remarkable extracellular level of pectinase performance. The
highest enzymatic activity in terms of halo zone diameters was achieved in
the case of cellulases and xylanases production by the isolates Mah 1a and
Mah 1b. Further research would be directed towards the production of the
tested enzymes by Bacillus spp. on the waste streams of food and
agroindustry, but also towards the utilization of the proven enzymatic
activity to obtain other microbial value-added products to develop viable
bioprocess solutions usable in the circular economy context. |
| first_indexed | 2024-03-09T00:07:00Z |
| format | Article |
| id | doaj.art-3b100f37db2441f4ba60c19595f8d0b2 |
| institution | Directory Open Access Journal |
| issn | 1450-7188 2406-095X |
| language | English |
| last_indexed | 2024-03-09T00:07:00Z |
| publishDate | 2023-01-01 |
| publisher | Faculty of Technology, Novi Sad |
| record_format | Article |
| series | Acta Periodica Technologica |
| spelling | doaj.art-3b100f37db2441f4ba60c19595f8d0b22023-12-12T13:06:35ZengFaculty of Technology, Novi SadActa Periodica Technologica1450-71882406-095X2023-01-0120235432533510.2298/APT2354325D1450-71882354325DBacillus spp. enzymatic activity to support circular economyDujković Tatjana D.0https://orcid.org/0009-0006-3096-7951Pajčin Ivana S.1Vlajkov Vanja R.2https://orcid.org/0000-0002-0585-3841Grahovac Jovana A.3https://orcid.org/0000-0001-9936-9665University of Novi Sad, Faculty of Technology Novi Sad, Novi Sad, SerbiaUniversity of Novi Sad, Faculty of Technology Novi Sad, Novi Sad, SerbiaUniversity of Novi Sad, Faculty of Technology Novi Sad, Novi Sad, SerbiaUniversity of Novi Sad, Faculty of Technology Novi Sad, Novi Sad, SerbiaAgricultural and industrial waste represents the most abundant renewable resource in the biosphere and is readily available in substantial quantities. Recently, there has been a growing focus on harnessing the potential of these types of waste as raw materials for producing value-added products, such as microbial enzymes, using solid-state or submerged fermentation systems. Among all the bacterial genera utilized in the production of industrially important enzymes, the Bacillus genus stands out as the most extensively employed for this purpose. In this study, the production of various enzymes such as protease, pectinase, cellulase, xylanase, lipase, and gelatinase was evaluated using the different Bacillus strains isolated from vegetables’ rhizosphere. The growth zones and halos were observed to determine production of the following enzymes: cellulases, proteases, pectinases, xylanases, gelatinases and lipases, confirming the breakdown of complex substrates in the medium. Bacillus Pap 3 exhibited the highest EAI (enzymatic activity index) value (4.00) for pectinase activity, indicating its remarkable extracellular level of pectinase performance. The highest enzymatic activity in terms of halo zone diameters was achieved in the case of cellulases and xylanases production by the isolates Mah 1a and Mah 1b. Further research would be directed towards the production of the tested enzymes by Bacillus spp. on the waste streams of food and agroindustry, but also towards the utilization of the proven enzymatic activity to obtain other microbial value-added products to develop viable bioprocess solutions usable in the circular economy context.https://doiserbia.nb.rs/img/doi/1450-7188/2023/1450-71882354325D.pdfcellulasesxylanasesproteases/gelatinasespectinaseslipases |
| spellingShingle | Dujković Tatjana D. Pajčin Ivana S. Vlajkov Vanja R. Grahovac Jovana A. Bacillus spp. enzymatic activity to support circular economy Acta Periodica Technologica cellulases xylanases proteases/gelatinases pectinases lipases |
| title | Bacillus spp. enzymatic activity to support circular economy |
| title_full | Bacillus spp. enzymatic activity to support circular economy |
| title_fullStr | Bacillus spp. enzymatic activity to support circular economy |
| title_full_unstemmed | Bacillus spp. enzymatic activity to support circular economy |
| title_short | Bacillus spp. enzymatic activity to support circular economy |
| title_sort | bacillus spp enzymatic activity to support circular economy |
| topic | cellulases xylanases proteases/gelatinases pectinases lipases |
| url | https://doiserbia.nb.rs/img/doi/1450-7188/2023/1450-71882354325D.pdf |
| work_keys_str_mv | AT dujkovictatjanad bacillussppenzymaticactivitytosupportcirculareconomy AT pajcinivanas bacillussppenzymaticactivitytosupportcirculareconomy AT vlajkovvanjar bacillussppenzymaticactivitytosupportcirculareconomy AT grahovacjovanaa bacillussppenzymaticactivitytosupportcirculareconomy |