Physiological responses to folate overproduction in <it>Lactobacillus plantarum </it>WCFS1
<p>Abstract</p> <p>Background</p> <p>Using a functional genomics approach we addressed the impact of folate overproduction on metabolite formation and gene expression in <it>Lactobacillus plantarum </it>WCFS1. We focused specifically on the mechanism that re...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2010-12-01
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| Series: | Microbial Cell Factories |
| Online Access: | http://www.microbialcellfactories.com/content/9/1/100 |
| _version_ | 1811246492441116672 |
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| author | de Vos Ric CH Molenaar Douwe Faijes Magda Mars Astrid E Wegkamp Arno Klaus Sebastian MJ Hanson Andrew D de Vos Willem M Smid Eddy J |
| author_facet | de Vos Ric CH Molenaar Douwe Faijes Magda Mars Astrid E Wegkamp Arno Klaus Sebastian MJ Hanson Andrew D de Vos Willem M Smid Eddy J |
| author_sort | de Vos Ric CH |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Using a functional genomics approach we addressed the impact of folate overproduction on metabolite formation and gene expression in <it>Lactobacillus plantarum </it>WCFS1. We focused specifically on the mechanism that reduces growth rates in folate-overproducing cells.</p> <p>Results</p> <p>Metabolite formation and gene expression were determined in a folate-overproducing- and wild-type strain. Differential metabolomics analysis of intracellular metabolite pools indicated that the pool sizes of 18 metabolites differed significantly between these strains. The gene expression profile was determined for both strains in pH-regulated chemostat culture and batch culture. Apart from the expected overexpression of the 6 genes of the folate gene cluster, no other genes were found to be differentially expressed both in continuous and batch cultures. The discrepancy between the low transcriptome and metabolome response and the 25% growth rate reduction of the folate overproducing strain was further investigated. Folate production per se could be ruled out as a contributing factor, since in the absence of folate production the growth rate of the overproducer was also reduced by 25%. The higher metabolic costs for DNA and RNA biosynthesis in the folate overproducing strain were also ruled out. However, it was demonstrated that folate-specific mRNAs and proteins constitute 8% and 4% of the total mRNA and protein pool, respectively.</p> <p>Conclusion</p> <p>Folate overproduction leads to very little change in metabolite levels or overall transcript profile, while at the same time the growth rate is reduced drastically. This shows that <it>Lactobacillus plantarum </it>WCFS1 is unable to respond to this growth rate reduction, most likely because the growth-related transcripts and proteins are diluted by the enormous amount of gratuitous folate-related transcripts and proteins.</p> |
| first_indexed | 2024-04-12T14:54:09Z |
| format | Article |
| id | doaj.art-3b1394978ec64790b85c0f038a6fea5b |
| institution | Directory Open Access Journal |
| issn | 1475-2859 |
| language | English |
| last_indexed | 2024-04-12T14:54:09Z |
| publishDate | 2010-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj.art-3b1394978ec64790b85c0f038a6fea5b2022-12-22T03:28:18ZengBMCMicrobial Cell Factories1475-28592010-12-019110010.1186/1475-2859-9-100Physiological responses to folate overproduction in <it>Lactobacillus plantarum </it>WCFS1de Vos Ric CHMolenaar DouweFaijes MagdaMars Astrid EWegkamp ArnoKlaus Sebastian MJHanson Andrew Dde Vos Willem MSmid Eddy J<p>Abstract</p> <p>Background</p> <p>Using a functional genomics approach we addressed the impact of folate overproduction on metabolite formation and gene expression in <it>Lactobacillus plantarum </it>WCFS1. We focused specifically on the mechanism that reduces growth rates in folate-overproducing cells.</p> <p>Results</p> <p>Metabolite formation and gene expression were determined in a folate-overproducing- and wild-type strain. Differential metabolomics analysis of intracellular metabolite pools indicated that the pool sizes of 18 metabolites differed significantly between these strains. The gene expression profile was determined for both strains in pH-regulated chemostat culture and batch culture. Apart from the expected overexpression of the 6 genes of the folate gene cluster, no other genes were found to be differentially expressed both in continuous and batch cultures. The discrepancy between the low transcriptome and metabolome response and the 25% growth rate reduction of the folate overproducing strain was further investigated. Folate production per se could be ruled out as a contributing factor, since in the absence of folate production the growth rate of the overproducer was also reduced by 25%. The higher metabolic costs for DNA and RNA biosynthesis in the folate overproducing strain were also ruled out. However, it was demonstrated that folate-specific mRNAs and proteins constitute 8% and 4% of the total mRNA and protein pool, respectively.</p> <p>Conclusion</p> <p>Folate overproduction leads to very little change in metabolite levels or overall transcript profile, while at the same time the growth rate is reduced drastically. This shows that <it>Lactobacillus plantarum </it>WCFS1 is unable to respond to this growth rate reduction, most likely because the growth-related transcripts and proteins are diluted by the enormous amount of gratuitous folate-related transcripts and proteins.</p>http://www.microbialcellfactories.com/content/9/1/100 |
| spellingShingle | de Vos Ric CH Molenaar Douwe Faijes Magda Mars Astrid E Wegkamp Arno Klaus Sebastian MJ Hanson Andrew D de Vos Willem M Smid Eddy J Physiological responses to folate overproduction in <it>Lactobacillus plantarum </it>WCFS1 Microbial Cell Factories |
| title | Physiological responses to folate overproduction in <it>Lactobacillus plantarum </it>WCFS1 |
| title_full | Physiological responses to folate overproduction in <it>Lactobacillus plantarum </it>WCFS1 |
| title_fullStr | Physiological responses to folate overproduction in <it>Lactobacillus plantarum </it>WCFS1 |
| title_full_unstemmed | Physiological responses to folate overproduction in <it>Lactobacillus plantarum </it>WCFS1 |
| title_short | Physiological responses to folate overproduction in <it>Lactobacillus plantarum </it>WCFS1 |
| title_sort | physiological responses to folate overproduction in it lactobacillus plantarum it wcfs1 |
| url | http://www.microbialcellfactories.com/content/9/1/100 |
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