Storage of plasma-derived exosomes: evaluation of anticoagulant use and preserving temperatures
AbstractExosomes carry large cargo of proteins, lipids, and nucleic acids, serving as versatile biomarkers for disease diagnosis and vehicles for drug delivery. However, up to date, no well recognized standard procedures for exosome storage were available for clinical application. This study aimed t...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Platelets |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/09537104.2024.2337255 |
| _version_ | 1797202379488100352 |
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| author | Caiting Yang Jie Han Hai Liu Yuyu He Zhenhua Zhang Xiaochun Liu Farooq Waqas Lizhong Zhang Huiping Duan Jing He Li Dong |
| author_facet | Caiting Yang Jie Han Hai Liu Yuyu He Zhenhua Zhang Xiaochun Liu Farooq Waqas Lizhong Zhang Huiping Duan Jing He Li Dong |
| author_sort | Caiting Yang |
| collection | DOAJ |
| description | AbstractExosomes carry large cargo of proteins, lipids, and nucleic acids, serving as versatile biomarkers for disease diagnosis and vehicles for drug delivery. However, up to date, no well recognized standard procedures for exosome storage were available for clinical application. This study aimed to determine the optimal storage conditions and the anticoagulants for plasma-derived exosome isolation. Fresh whole blood samples were collected from healthy participants and preserved in four different anticoagulants including sodium citrate (SC1/4), sodium citrate (SC1/9), lithium heparin (LH), or Ethylenediamine tetraacetic acid (EDTA), respectively. Exosomes were extracted from the plasma by differential ultracentrifugation and stored at three different temperatures, 4°C, −20°C or − 80°C for a duration ranging from one week to six months. All plasma samples for storage conditions comparison were pretreated with LH anticoagulant. Exosome features including morphological characteristics, pariticles size diameter, and surface protein profiles (TSG101, CD63, CD81, CD9, CALNEXIN) were assessed by transmission electron microscopy, Nanoparticle Tracking Analysis, and Western Blotting, respectively. Exosomes preserved in LH and SC1/4 group tended to remain intact microstructure with highly abundant protein biomarkers. Exosomes stored at 4°C for short time were prone to be more stable compared to thos at −80°C. Exosomes stored in plasma were superior in terms of ultrastructure, size diameter and surface protein expression to those stored in PBS. In conclusion, plasma-dervied exosome characteristics strictly depend on the anticoagulants and storage temperature and duration. |
| first_indexed | 2024-04-24T08:02:30Z |
| format | Article |
| id | doaj.art-3b13b13584d841349a22ad8c31176841 |
| institution | Directory Open Access Journal |
| issn | 0953-7104 1369-1635 |
| language | English |
| last_indexed | 2024-04-24T08:02:30Z |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Platelets |
| spelling | doaj.art-3b13b13584d841349a22ad8c311768412024-04-17T14:24:41ZengTaylor & Francis GroupPlatelets0953-71041369-16352024-12-0135110.1080/09537104.2024.2337255Storage of plasma-derived exosomes: evaluation of anticoagulant use and preserving temperaturesCaiting Yang0Jie Han1Hai Liu2Yuyu He3Zhenhua Zhang4Xiaochun Liu5Farooq Waqas6Lizhong Zhang7Huiping Duan8Jing He9Li Dong10Shanxi Provincial Key Laboratory for Medical Molecular Cell Biology, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education and Institute of Biomedical Sciences, Shanxi University, Taiyuan, ChinaCenter for Biomechanics and Bioengineering, Key Laboratory of Microgravity (National Microgravity Laboratory), and Beijing Key Laboratory of Engineered Construction and Mechanobiology, Institute of Mechanics, Chinese Academy of Sciences, Beijing, ChinaShanxi Provincial Key Laboratory for Medical Molecular Cell Biology, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education and Institute of Biomedical Sciences, Shanxi University, Taiyuan, ChinaShanxi Provincial Key Laboratory for Medical Molecular Cell Biology, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education and Institute of Biomedical Sciences, Shanxi University, Taiyuan, ChinaShanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital, Third Hospital of Shanxi Medical University, Taiyuan, ChinaShanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital, Third Hospital of Shanxi Medical University, Taiyuan, ChinaShanxi Provincial Key Laboratory for Medical Molecular Cell Biology, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education and Institute of Biomedical Sciences, Shanxi University, Taiyuan, ChinaShanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital, Third Hospital of Shanxi Medical University, Taiyuan, ChinaTuberculosis Department, The Fourth People’s Hospital of Taiyuan, Taiyuan, ChinaShanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital, Third Hospital of Shanxi Medical University, Taiyuan, ChinaShanxi Provincial Key Laboratory for Medical Molecular Cell Biology, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education and Institute of Biomedical Sciences, Shanxi University, Taiyuan, ChinaAbstractExosomes carry large cargo of proteins, lipids, and nucleic acids, serving as versatile biomarkers for disease diagnosis and vehicles for drug delivery. However, up to date, no well recognized standard procedures for exosome storage were available for clinical application. This study aimed to determine the optimal storage conditions and the anticoagulants for plasma-derived exosome isolation. Fresh whole blood samples were collected from healthy participants and preserved in four different anticoagulants including sodium citrate (SC1/4), sodium citrate (SC1/9), lithium heparin (LH), or Ethylenediamine tetraacetic acid (EDTA), respectively. Exosomes were extracted from the plasma by differential ultracentrifugation and stored at three different temperatures, 4°C, −20°C or − 80°C for a duration ranging from one week to six months. All plasma samples for storage conditions comparison were pretreated with LH anticoagulant. Exosome features including morphological characteristics, pariticles size diameter, and surface protein profiles (TSG101, CD63, CD81, CD9, CALNEXIN) were assessed by transmission electron microscopy, Nanoparticle Tracking Analysis, and Western Blotting, respectively. Exosomes preserved in LH and SC1/4 group tended to remain intact microstructure with highly abundant protein biomarkers. Exosomes stored at 4°C for short time were prone to be more stable compared to thos at −80°C. Exosomes stored in plasma were superior in terms of ultrastructure, size diameter and surface protein expression to those stored in PBS. In conclusion, plasma-dervied exosome characteristics strictly depend on the anticoagulants and storage temperature and duration.https://www.tandfonline.com/doi/10.1080/09537104.2024.2337255exosomeanticoagulantplasmatemperatureduration |
| spellingShingle | Caiting Yang Jie Han Hai Liu Yuyu He Zhenhua Zhang Xiaochun Liu Farooq Waqas Lizhong Zhang Huiping Duan Jing He Li Dong Storage of plasma-derived exosomes: evaluation of anticoagulant use and preserving temperatures Platelets exosome anticoagulant plasma temperature duration |
| title | Storage of plasma-derived exosomes: evaluation of anticoagulant use and preserving temperatures |
| title_full | Storage of plasma-derived exosomes: evaluation of anticoagulant use and preserving temperatures |
| title_fullStr | Storage of plasma-derived exosomes: evaluation of anticoagulant use and preserving temperatures |
| title_full_unstemmed | Storage of plasma-derived exosomes: evaluation of anticoagulant use and preserving temperatures |
| title_short | Storage of plasma-derived exosomes: evaluation of anticoagulant use and preserving temperatures |
| title_sort | storage of plasma derived exosomes evaluation of anticoagulant use and preserving temperatures |
| topic | exosome anticoagulant plasma temperature duration |
| url | https://www.tandfonline.com/doi/10.1080/09537104.2024.2337255 |
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