Cloning and Sequence Analysis of Gene Encoding psbZ from Silybum marianum (L.) Gaertn
Photosystem II is known as the core of photosynthesis and among of the photosystem II protein complex, PsbZ protein is very important because of its role in connection PSII and LHCII. For identifying and sequencing of the psbZ gene in Silybum marianum plant, seeds were prepared from botanical garden...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Bulgarian Academy of Sciences
2016-09-01
|
| Series: | International Journal Bioautomation |
| Subjects: | |
| Online Access: | http://www.biomed.bas.bg/bioautomation/2016/vol_20.3/files/20.3_04.pdf |
| _version_ | 1818160229700861952 |
|---|---|
| author | Yousef Mohammadi Ahmad Razban Haghighi Shiva Gheytaranpour Sehrigh |
| author_facet | Yousef Mohammadi Ahmad Razban Haghighi Shiva Gheytaranpour Sehrigh |
| author_sort | Yousef Mohammadi |
| collection | DOAJ |
| description | Photosystem II is known as the core of photosynthesis and among of the photosystem II protein complex, PsbZ protein is very important because of its role in connection PSII and LHCII. For identifying and sequencing of the psbZ gene in Silybum marianum plant, seeds were prepared from botanical garden in Tabriz, Iran. DNA was extracted by CTAB method and by using the PCR amplification the fragment length was estimated to be 705 bp. The amplified fragment was cloned in pGEM-T vector and E.coli transformation was carried out. The plasmid was extracted and the sequence was recorded at NCBI with accession number of GQ225868. BLAST of psbZ, glycine tRNA and psbZ-tRNA GLY intergenic spacer showed that each region is highly conserved among species. BLAST of predicted PsbZ protein also represents a severe conservation among species. Because of the importance of PsbZ protein in photosynthesis, it is necessary to create targeted mutations to determine the active sites of proteins. |
| first_indexed | 2024-12-11T15:58:34Z |
| format | Article |
| id | doaj.art-3b193b5c161e4401aaac614df28db1bd |
| institution | Directory Open Access Journal |
| issn | 1314-1902 1314-2321 |
| language | English |
| last_indexed | 2024-12-11T15:58:34Z |
| publishDate | 2016-09-01 |
| publisher | Bulgarian Academy of Sciences |
| record_format | Article |
| series | International Journal Bioautomation |
| spelling | doaj.art-3b193b5c161e4401aaac614df28db1bd2022-12-22T00:59:22ZengBulgarian Academy of SciencesInternational Journal Bioautomation1314-19021314-23212016-09-01203351358Cloning and Sequence Analysis of Gene Encoding psbZ from Silybum marianum (L.) GaertnYousef Mohammadi0Ahmad Razban HaghighiShiva Gheytaranpour SehrighDeptartment of Plant Breeding and Biotechnology, University of Tabriz, IranPhotosystem II is known as the core of photosynthesis and among of the photosystem II protein complex, PsbZ protein is very important because of its role in connection PSII and LHCII. For identifying and sequencing of the psbZ gene in Silybum marianum plant, seeds were prepared from botanical garden in Tabriz, Iran. DNA was extracted by CTAB method and by using the PCR amplification the fragment length was estimated to be 705 bp. The amplified fragment was cloned in pGEM-T vector and E.coli transformation was carried out. The plasmid was extracted and the sequence was recorded at NCBI with accession number of GQ225868. BLAST of psbZ, glycine tRNA and psbZ-tRNA GLY intergenic spacer showed that each region is highly conserved among species. BLAST of predicted PsbZ protein also represents a severe conservation among species. Because of the importance of PsbZ protein in photosynthesis, it is necessary to create targeted mutations to determine the active sites of proteins.http://www.biomed.bas.bg/bioautomation/2016/vol_20.3/files/20.3_04.pdfChloroplastPhotosynthesisPhotosystemIISequencingTransformationSilybum marianum |
| spellingShingle | Yousef Mohammadi Ahmad Razban Haghighi Shiva Gheytaranpour Sehrigh Cloning and Sequence Analysis of Gene Encoding psbZ from Silybum marianum (L.) Gaertn International Journal Bioautomation Chloroplast Photosynthesis PhotosystemII Sequencing Transformation Silybum marianum |
| title | Cloning and Sequence Analysis of Gene Encoding psbZ from Silybum marianum (L.) Gaertn |
| title_full | Cloning and Sequence Analysis of Gene Encoding psbZ from Silybum marianum (L.) Gaertn |
| title_fullStr | Cloning and Sequence Analysis of Gene Encoding psbZ from Silybum marianum (L.) Gaertn |
| title_full_unstemmed | Cloning and Sequence Analysis of Gene Encoding psbZ from Silybum marianum (L.) Gaertn |
| title_short | Cloning and Sequence Analysis of Gene Encoding psbZ from Silybum marianum (L.) Gaertn |
| title_sort | cloning and sequence analysis of gene encoding psbz from silybum marianum l gaertn |
| topic | Chloroplast Photosynthesis PhotosystemII Sequencing Transformation Silybum marianum |
| url | http://www.biomed.bas.bg/bioautomation/2016/vol_20.3/files/20.3_04.pdf |
| work_keys_str_mv | AT yousefmohammadi cloningandsequenceanalysisofgeneencodingpsbzfromsilybummarianumlgaertn AT ahmadrazbanhaghighi cloningandsequenceanalysisofgeneencodingpsbzfromsilybummarianumlgaertn AT shivagheytaranpoursehrigh cloningandsequenceanalysisofgeneencodingpsbzfromsilybummarianumlgaertn |