Assays to Monitor Autophagy Progression in Cell Cultures
The vast number of implications of autophagy in multiple areas of life sciences and medicine has attracted the interest of numerous scientists that aim to unveil the role of this process in specific physiological and pathological contexts. Cell cultures are one of the most frequently used experiment...
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| Format: | Article |
| Language: | English |
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MDPI AG
2017-07-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/6/3/20 |
| _version_ | 1797763766230712320 |
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| author | Idil Orhon Fulvio Reggiori |
| author_facet | Idil Orhon Fulvio Reggiori |
| author_sort | Idil Orhon |
| collection | DOAJ |
| description | The vast number of implications of autophagy in multiple areas of life sciences and medicine has attracted the interest of numerous scientists that aim to unveil the role of this process in specific physiological and pathological contexts. Cell cultures are one of the most frequently used experimental setup for the investigation of autophagy. As a result, it is essential to assess this highly regulated molecular pathway with efficient and reliable methods. Each method has its own advantages and disadvantages. Here, we present a review summarizing the most established assays used to monitor autophagy induction and progression in cell cultures, in order to guide researchers in the selection of the most optimal solution for their experimental setup and design. |
| first_indexed | 2024-03-12T19:47:02Z |
| format | Article |
| id | doaj.art-3b1a0a7e400045ea9b715c2228c2c6cd |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-12T19:47:02Z |
| publishDate | 2017-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-3b1a0a7e400045ea9b715c2228c2c6cd2023-08-02T03:28:21ZengMDPI AGCells2073-44092017-07-01632010.3390/cells6030020cells6030020Assays to Monitor Autophagy Progression in Cell CulturesIdil Orhon0Fulvio Reggiori1Department of Cell Biology, University of Groningen, University Medical Center Groningen, A. Deusinglaan 1, 9713 AV Groningen, The NetherlandsDepartment of Cell Biology, University of Groningen, University Medical Center Groningen, A. Deusinglaan 1, 9713 AV Groningen, The NetherlandsThe vast number of implications of autophagy in multiple areas of life sciences and medicine has attracted the interest of numerous scientists that aim to unveil the role of this process in specific physiological and pathological contexts. Cell cultures are one of the most frequently used experimental setup for the investigation of autophagy. As a result, it is essential to assess this highly regulated molecular pathway with efficient and reliable methods. Each method has its own advantages and disadvantages. Here, we present a review summarizing the most established assays used to monitor autophagy induction and progression in cell cultures, in order to guide researchers in the selection of the most optimal solution for their experimental setup and design.https://www.mdpi.com/2073-4409/6/3/20autophagyautophagosomephagophoreautophagic fluxmeasurementassaymethod |
| spellingShingle | Idil Orhon Fulvio Reggiori Assays to Monitor Autophagy Progression in Cell Cultures Cells autophagy autophagosome phagophore autophagic flux measurement assay method |
| title | Assays to Monitor Autophagy Progression in Cell Cultures |
| title_full | Assays to Monitor Autophagy Progression in Cell Cultures |
| title_fullStr | Assays to Monitor Autophagy Progression in Cell Cultures |
| title_full_unstemmed | Assays to Monitor Autophagy Progression in Cell Cultures |
| title_short | Assays to Monitor Autophagy Progression in Cell Cultures |
| title_sort | assays to monitor autophagy progression in cell cultures |
| topic | autophagy autophagosome phagophore autophagic flux measurement assay method |
| url | https://www.mdpi.com/2073-4409/6/3/20 |
| work_keys_str_mv | AT idilorhon assaystomonitorautophagyprogressionincellcultures AT fulvioreggiori assaystomonitorautophagyprogressionincellcultures |