Trends in Asymptomatic Nasopharyngeal <i>Streptococcus pneumoniae</i> Carriage with qPCR and Culture Analysis
We previously reported trends in pneumococcal nasopharyngeal carriage in the post-PCV13 era as detected by conventional culture methods. Our current aim is to assess if there are fundamental differences in the clinical and demographic features of children who have pneumococcal carriage detected by q...
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| Format: | Article |
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MDPI AG
2022-10-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/10/2074 |
| _version_ | 1797471059656572928 |
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| author | Julie-Anne Lemay Leah J. Ricketson James D. Kellner |
| author_facet | Julie-Anne Lemay Leah J. Ricketson James D. Kellner |
| author_sort | Julie-Anne Lemay |
| collection | DOAJ |
| description | We previously reported trends in pneumococcal nasopharyngeal carriage in the post-PCV13 era as detected by conventional culture methods. Our current aim is to assess if there are fundamental differences in the clinical and demographic features of children who have pneumococcal carriage detected by qPCR compared with culture analysis. The CASPER team conducted point-prevalence surveys in 2016 in healthy children in Calgary to determine trends in overall and serotype-specific pneumococcal nasopharyngeal carriage. Being 18 months of age (<i>p</i> = 0.009), having at least one sibling under 2 years of age (<i>p</i> = 0.04), having only sibling(s) over 2 years of age (<i>p</i> = 0.001), and childcare attendance (<i>p</i> = 0.005) were associated with carriage by qPCR methods only. Having only sibling(s) older than 2 years of age was associated with carriage detected by both qPCR and culture methods (<i>p</i> = 0.001). No clinical factors were associated with carriage detected by both qPCR and culture compared to qPCR methods only. Both analyses are suitable methods to detect carriage; however, qPCR analysis is more sensitive and more cost-effective. As there are no fundamental differences in the children that have pneumococcal nasopharyngeal carriage detectable by qPCR methods compared to conventional culture methods, molecular analysis may be a preferable option for future carriage studies. |
| first_indexed | 2024-03-09T19:44:14Z |
| format | Article |
| id | doaj.art-3b1f2b5d036b43fb9d4468b9a7abad8c |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T19:44:14Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-3b1f2b5d036b43fb9d4468b9a7abad8c2023-11-24T01:27:59ZengMDPI AGMicroorganisms2076-26072022-10-011010207410.3390/microorganisms10102074Trends in Asymptomatic Nasopharyngeal <i>Streptococcus pneumoniae</i> Carriage with qPCR and Culture AnalysisJulie-Anne Lemay0Leah J. Ricketson1James D. Kellner2Department of Pediatrics, University of Calgary, Calgary, AB T2N 1N4, CanadaDepartment of Pediatrics, University of Calgary, Calgary, AB T2N 1N4, CanadaDepartment of Pediatrics, University of Calgary, Calgary, AB T2N 1N4, CanadaWe previously reported trends in pneumococcal nasopharyngeal carriage in the post-PCV13 era as detected by conventional culture methods. Our current aim is to assess if there are fundamental differences in the clinical and demographic features of children who have pneumococcal carriage detected by qPCR compared with culture analysis. The CASPER team conducted point-prevalence surveys in 2016 in healthy children in Calgary to determine trends in overall and serotype-specific pneumococcal nasopharyngeal carriage. Being 18 months of age (<i>p</i> = 0.009), having at least one sibling under 2 years of age (<i>p</i> = 0.04), having only sibling(s) over 2 years of age (<i>p</i> = 0.001), and childcare attendance (<i>p</i> = 0.005) were associated with carriage by qPCR methods only. Having only sibling(s) older than 2 years of age was associated with carriage detected by both qPCR and culture methods (<i>p</i> = 0.001). No clinical factors were associated with carriage detected by both qPCR and culture compared to qPCR methods only. Both analyses are suitable methods to detect carriage; however, qPCR analysis is more sensitive and more cost-effective. As there are no fundamental differences in the children that have pneumococcal nasopharyngeal carriage detectable by qPCR methods compared to conventional culture methods, molecular analysis may be a preferable option for future carriage studies.https://www.mdpi.com/2076-2607/10/10/2074<i>Streptococcus pneumoniae</i>13-valent pneumococcal conjugate vaccinenasopharyngeal carriage |
| spellingShingle | Julie-Anne Lemay Leah J. Ricketson James D. Kellner Trends in Asymptomatic Nasopharyngeal <i>Streptococcus pneumoniae</i> Carriage with qPCR and Culture Analysis Microorganisms <i>Streptococcus pneumoniae</i> 13-valent pneumococcal conjugate vaccine nasopharyngeal carriage |
| title | Trends in Asymptomatic Nasopharyngeal <i>Streptococcus pneumoniae</i> Carriage with qPCR and Culture Analysis |
| title_full | Trends in Asymptomatic Nasopharyngeal <i>Streptococcus pneumoniae</i> Carriage with qPCR and Culture Analysis |
| title_fullStr | Trends in Asymptomatic Nasopharyngeal <i>Streptococcus pneumoniae</i> Carriage with qPCR and Culture Analysis |
| title_full_unstemmed | Trends in Asymptomatic Nasopharyngeal <i>Streptococcus pneumoniae</i> Carriage with qPCR and Culture Analysis |
| title_short | Trends in Asymptomatic Nasopharyngeal <i>Streptococcus pneumoniae</i> Carriage with qPCR and Culture Analysis |
| title_sort | trends in asymptomatic nasopharyngeal i streptococcus pneumoniae i carriage with qpcr and culture analysis |
| topic | <i>Streptococcus pneumoniae</i> 13-valent pneumococcal conjugate vaccine nasopharyngeal carriage |
| url | https://www.mdpi.com/2076-2607/10/10/2074 |
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