Sulphate Reducing Bacteria in Biofilms on Thermosetting Polymers/Zn Composite Layers

Bacterial adhesion to surfaces is the first step in the formation of a biofilm and has been studied extensively over the past decades in many diverse applications. Sulphate Reducing Bacteria (SRB) is a group of phylogenetically diverse anaerobic microorganisms that were first discovered by Beijerinc...

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Main Authors: Alina CIUBOTARIU, Lidia BENEA, Wolfgang SAND
Format: Article
Language:English
Published: Galati University Press 2011-03-01
Series:The Annals of “Dunarea de Jos” University of Galati. Fascicle IX, Metallurgy and Materials Science
Subjects:
Online Access:https://www.gup.ugal.ro/ugaljournals/index.php/mms/article/view/2967
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author Alina CIUBOTARIU
Lidia BENEA
Wolfgang SAND
author_facet Alina CIUBOTARIU
Lidia BENEA
Wolfgang SAND
author_sort Alina CIUBOTARIU
collection DOAJ
description Bacterial adhesion to surfaces is the first step in the formation of a biofilm and has been studied extensively over the past decades in many diverse applications. Sulphate Reducing Bacteria (SRB) is a group of phylogenetically diverse anaerobic microorganisms that were first discovered by Beijerinck in 1895. This work investigates the attachment of Sulphate Reducing Bacteria and the modification of roughness before and after the attachments on the surfaces of zinc and thermosetting polymers/zinc composite layers obtained by electro co-deposition. There were used two types of thermosetting polymers: phenol – formaldehyde resin (type NOVOLAC) and epoxi resin. For investigations of the surfaces were used atomic force and epifluorescence microscopy methods (AFM and EFM, respectively). Sessile bacteria on coupons were stained with 4’, 6-diamidino-2-phenylindol (DAPI) and visualized by EFM as well as AFM. The best imaging conditions for AFM were assessed.
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spelling doaj.art-3b229b2cf3794c78a25ff2baf9d687072022-12-21T20:13:45ZengGalati University PressThe Annals of “Dunarea de Jos” University of Galati. Fascicle IX, Metallurgy and Materials Science2668-47482668-47562011-03-013412967Sulphate Reducing Bacteria in Biofilms on Thermosetting Polymers/Zn Composite LayersAlina CIUBOTARIU0Lidia BENEA1Wolfgang SAND2"Dunarea de Jos" University of Galati, Romania"Dunarea de Jos" University of Galati, RomaniaUniversity of Duisburg Essen, Biofilm Centre, Aquatic Biotechnology, GermanyBacterial adhesion to surfaces is the first step in the formation of a biofilm and has been studied extensively over the past decades in many diverse applications. Sulphate Reducing Bacteria (SRB) is a group of phylogenetically diverse anaerobic microorganisms that were first discovered by Beijerinck in 1895. This work investigates the attachment of Sulphate Reducing Bacteria and the modification of roughness before and after the attachments on the surfaces of zinc and thermosetting polymers/zinc composite layers obtained by electro co-deposition. There were used two types of thermosetting polymers: phenol – formaldehyde resin (type NOVOLAC) and epoxi resin. For investigations of the surfaces were used atomic force and epifluorescence microscopy methods (AFM and EFM, respectively). Sessile bacteria on coupons were stained with 4’, 6-diamidino-2-phenylindol (DAPI) and visualized by EFM as well as AFM. The best imaging conditions for AFM were assessed.https://www.gup.ugal.ro/ugaljournals/index.php/mms/article/view/2967biofilmcomposite layersSulphate Reducing BacteriaAtomic Force MicroscopyEpifluorescence Microscopyroughness
spellingShingle Alina CIUBOTARIU
Lidia BENEA
Wolfgang SAND
Sulphate Reducing Bacteria in Biofilms on Thermosetting Polymers/Zn Composite Layers
The Annals of “Dunarea de Jos” University of Galati. Fascicle IX, Metallurgy and Materials Science
biofilm
composite layers
Sulphate Reducing Bacteria
Atomic Force Microscopy
Epifluorescence Microscopy
roughness
title Sulphate Reducing Bacteria in Biofilms on Thermosetting Polymers/Zn Composite Layers
title_full Sulphate Reducing Bacteria in Biofilms on Thermosetting Polymers/Zn Composite Layers
title_fullStr Sulphate Reducing Bacteria in Biofilms on Thermosetting Polymers/Zn Composite Layers
title_full_unstemmed Sulphate Reducing Bacteria in Biofilms on Thermosetting Polymers/Zn Composite Layers
title_short Sulphate Reducing Bacteria in Biofilms on Thermosetting Polymers/Zn Composite Layers
title_sort sulphate reducing bacteria in biofilms on thermosetting polymers zn composite layers
topic biofilm
composite layers
Sulphate Reducing Bacteria
Atomic Force Microscopy
Epifluorescence Microscopy
roughness
url https://www.gup.ugal.ro/ugaljournals/index.php/mms/article/view/2967
work_keys_str_mv AT alinaciubotariu sulphatereducingbacteriainbiofilmsonthermosettingpolymerszncompositelayers
AT lidiabenea sulphatereducingbacteriainbiofilmsonthermosettingpolymerszncompositelayers
AT wolfgangsand sulphatereducingbacteriainbiofilmsonthermosettingpolymerszncompositelayers