Genetic similarity of Burkholderia cenocepacia from cystic fibrosis patients
Burkholderia cenocepacia may cause serious infections in patients with cystic fibrosis, and this microorganism can be highly transmissible. Pulsed-field gel electrophoresis is widely used to study the dynamics of strain spread in cystic fibrosis patients. The aim of this work was to perform pulsed-f...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2013-02-01
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| Series: | Brazilian Journal of Infectious Diseases |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000100014 |
| _version_ | 1828400602913177600 |
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| author | Luana Pretto Fernanda de-Paris Alice Beatriz Mombach Pinheiro Machado Andreza Francisco Martins Afonso Luís Barth |
| author_facet | Luana Pretto Fernanda de-Paris Alice Beatriz Mombach Pinheiro Machado Andreza Francisco Martins Afonso Luís Barth |
| author_sort | Luana Pretto |
| collection | DOAJ |
| description | Burkholderia cenocepacia may cause serious infections in patients with cystic fibrosis, and this microorganism can be highly transmissible. Pulsed-field gel electrophoresis is widely used to study the dynamics of strain spread in cystic fibrosis patients. The aim of this work was to perform pulsed-field gel electrophoresis-based molecular typing of B. cenocepacia isolates to evaluate the epidemiology of this species at our hospital. A total of 28 isolates from 23 cystic fibrosis patients were analyzed. Initially, we compared isolates obtained from the same patient at different periods of time. We then compared the pulsed-field gel electrophoresis profiles of 15 IIIA isolates, and in a third analysis, evaluated the genetic profile of 8 IIIB isolates from different patients. The pulsed-field gel electrophoresis profiles of isolates from the same patient indicated that they are genetically indistinguishable. Analysis of isolates from different patients revealed the presence of multiple clonal groups. These results do not indicate cross-transmission of a unique clone of B. cenocepacia among cystic fibrosis patients, although this has been observed in some patients. Our findings highlight the importance of adequate patient follow-up at cystic fibrosis centers and adherence to management and segregation measures in cystic fibrosis patients colonized with B. cenocepacia. |
| first_indexed | 2024-12-10T09:34:40Z |
| format | Article |
| id | doaj.art-3b24125fd0404c6780cfe814f82992c4 |
| institution | Directory Open Access Journal |
| issn | 1413-8670 1678-4391 |
| language | English |
| last_indexed | 2024-12-10T09:34:40Z |
| publishDate | 2013-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Brazilian Journal of Infectious Diseases |
| spelling | doaj.art-3b24125fd0404c6780cfe814f82992c42022-12-22T01:54:13ZengElsevierBrazilian Journal of Infectious Diseases1413-86701678-43912013-02-011718689Genetic similarity of Burkholderia cenocepacia from cystic fibrosis patientsLuana PrettoFernanda de-ParisAlice Beatriz Mombach Pinheiro MachadoAndreza Francisco MartinsAfonso Luís BarthBurkholderia cenocepacia may cause serious infections in patients with cystic fibrosis, and this microorganism can be highly transmissible. Pulsed-field gel electrophoresis is widely used to study the dynamics of strain spread in cystic fibrosis patients. The aim of this work was to perform pulsed-field gel electrophoresis-based molecular typing of B. cenocepacia isolates to evaluate the epidemiology of this species at our hospital. A total of 28 isolates from 23 cystic fibrosis patients were analyzed. Initially, we compared isolates obtained from the same patient at different periods of time. We then compared the pulsed-field gel electrophoresis profiles of 15 IIIA isolates, and in a third analysis, evaluated the genetic profile of 8 IIIB isolates from different patients. The pulsed-field gel electrophoresis profiles of isolates from the same patient indicated that they are genetically indistinguishable. Analysis of isolates from different patients revealed the presence of multiple clonal groups. These results do not indicate cross-transmission of a unique clone of B. cenocepacia among cystic fibrosis patients, although this has been observed in some patients. Our findings highlight the importance of adequate patient follow-up at cystic fibrosis centers and adherence to management and segregation measures in cystic fibrosis patients colonized with B. cenocepacia.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000100014Burkholderia cenocepaciaCystic fibrosisPFGEGenetic similarity |
| spellingShingle | Luana Pretto Fernanda de-Paris Alice Beatriz Mombach Pinheiro Machado Andreza Francisco Martins Afonso Luís Barth Genetic similarity of Burkholderia cenocepacia from cystic fibrosis patients Brazilian Journal of Infectious Diseases Burkholderia cenocepacia Cystic fibrosis PFGE Genetic similarity |
| title | Genetic similarity of Burkholderia cenocepacia from cystic fibrosis patients |
| title_full | Genetic similarity of Burkholderia cenocepacia from cystic fibrosis patients |
| title_fullStr | Genetic similarity of Burkholderia cenocepacia from cystic fibrosis patients |
| title_full_unstemmed | Genetic similarity of Burkholderia cenocepacia from cystic fibrosis patients |
| title_short | Genetic similarity of Burkholderia cenocepacia from cystic fibrosis patients |
| title_sort | genetic similarity of burkholderia cenocepacia from cystic fibrosis patients |
| topic | Burkholderia cenocepacia Cystic fibrosis PFGE Genetic similarity |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000100014 |
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