Molecular characterization of dengue virus reveals regional diversification of serotype 2 in Colombia
Abstract Dengue is hyperendemic in Colombia, where a cyclic behavior of serotype replacement leading to periodic epidemics has been observed for decades. This level of endemicity favors accumulation of dengue virus genetic diversity and could be linked to disease outcome. To assess the genetic diver...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-05-01
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| Series: | Virology Journal |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12985-019-1170-4 |
| _version_ | 1818525379304882176 |
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| author | Katherine Laiton-Donato Diego A. Alvarez Dioselina Peláez-Carvajal Marcela Mercado Nadim J. Ajami Irene Bosch José A. Usme-Ciro |
| author_facet | Katherine Laiton-Donato Diego A. Alvarez Dioselina Peláez-Carvajal Marcela Mercado Nadim J. Ajami Irene Bosch José A. Usme-Ciro |
| author_sort | Katherine Laiton-Donato |
| collection | DOAJ |
| description | Abstract Dengue is hyperendemic in Colombia, where a cyclic behavior of serotype replacement leading to periodic epidemics has been observed for decades. This level of endemicity favors accumulation of dengue virus genetic diversity and could be linked to disease outcome. To assess the genetic diversity of dengue virus type 2 in Colombia, we sequenced the envelope gene of 24 virus isolates from acute cases of dengue or severe dengue fever during the period 2013–2016. The phylogenetic analysis revealed the circulation of the Asian-American genotype of dengue virus type 2 in Colombia during that period, the intra-genotype variability leading to divergence in two recently circulating lineages with differential geographic distribution, as well as the presence of nonsynonymous substitutions accompanying their emergence and diversification. |
| first_indexed | 2024-12-11T06:08:35Z |
| format | Article |
| id | doaj.art-3b265e9f2e1e49c487fad6ed484c2312 |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-12-11T06:08:35Z |
| publishDate | 2019-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-3b265e9f2e1e49c487fad6ed484c23122022-12-22T01:18:13ZengBMCVirology Journal1743-422X2019-05-011611710.1186/s12985-019-1170-4Molecular characterization of dengue virus reveals regional diversification of serotype 2 in ColombiaKatherine Laiton-Donato0Diego A. Alvarez1Dioselina Peláez-Carvajal2Marcela Mercado3Nadim J. Ajami4Irene Bosch5José A. Usme-Ciro6Grupo de Virología, Dirección de Redes en Salud Pública, Instituto Nacional de SaludGrupo de Virología, Dirección de Redes en Salud Pública, Instituto Nacional de SaludGrupo de Virología, Dirección de Redes en Salud Pública, Instituto Nacional de SaludDirección de Vigilancia y Análisis del Riesgo en Salud Pública, Instituto Nacional de SaludAlkek Center for Metagenomics and Microbiome Research, Baylor College of MedicineInstitute for Medical Engineering and Science, Massachusetts Institute of TechnologyGrupo de Virología, Dirección de Redes en Salud Pública, Instituto Nacional de SaludAbstract Dengue is hyperendemic in Colombia, where a cyclic behavior of serotype replacement leading to periodic epidemics has been observed for decades. This level of endemicity favors accumulation of dengue virus genetic diversity and could be linked to disease outcome. To assess the genetic diversity of dengue virus type 2 in Colombia, we sequenced the envelope gene of 24 virus isolates from acute cases of dengue or severe dengue fever during the period 2013–2016. The phylogenetic analysis revealed the circulation of the Asian-American genotype of dengue virus type 2 in Colombia during that period, the intra-genotype variability leading to divergence in two recently circulating lineages with differential geographic distribution, as well as the presence of nonsynonymous substitutions accompanying their emergence and diversification.http://link.springer.com/article/10.1186/s12985-019-1170-4Dengue virusMolecular characterizationPhylogenyEnvelopeEvolution |
| spellingShingle | Katherine Laiton-Donato Diego A. Alvarez Dioselina Peláez-Carvajal Marcela Mercado Nadim J. Ajami Irene Bosch José A. Usme-Ciro Molecular characterization of dengue virus reveals regional diversification of serotype 2 in Colombia Virology Journal Dengue virus Molecular characterization Phylogeny Envelope Evolution |
| title | Molecular characterization of dengue virus reveals regional diversification of serotype 2 in Colombia |
| title_full | Molecular characterization of dengue virus reveals regional diversification of serotype 2 in Colombia |
| title_fullStr | Molecular characterization of dengue virus reveals regional diversification of serotype 2 in Colombia |
| title_full_unstemmed | Molecular characterization of dengue virus reveals regional diversification of serotype 2 in Colombia |
| title_short | Molecular characterization of dengue virus reveals regional diversification of serotype 2 in Colombia |
| title_sort | molecular characterization of dengue virus reveals regional diversification of serotype 2 in colombia |
| topic | Dengue virus Molecular characterization Phylogeny Envelope Evolution |
| url | http://link.springer.com/article/10.1186/s12985-019-1170-4 |
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