Preservation and maintenance of Pseudomonas aeruginosa for use in the laboratory
The purpose of preserving and maintaining bacterial cultures in a university setting is to ensure they remain viable for laboratory classes and research, and generate consistent laboratory test results over time. Pseudomonas aeruginosa can be preserved by refrigeration at 4°C, but this is only suita...
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| Format: | Article |
| Language: | Thai |
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Mahasarakham University
2019-02-01
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| Series: | Warasan Witthayasat Lae Theknoloyi Mahawitthayalai Mahasarakham |
| Subjects: | |
| Online Access: | http://journal.msu.ac.th/upload/articles/article2427_80695.pdf |
| _version_ | 1818021044845281280 |
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| author | Sujitra Yahom |
| author_facet | Sujitra Yahom |
| author_sort | Sujitra Yahom |
| collection | DOAJ |
| description | The purpose of preserving and maintaining bacterial cultures in a university setting is to ensure they remain viable for laboratory classes and research, and generate consistent laboratory test results over time. Pseudomonas aeruginosa can be preserved by refrigeration at 4°C, but this is only suitable for short-term storage (2-3 weeks). Longer-term storage can be achieved if the bacteria being stored at 4°C are periodically sub-cultured. This is known as the
‘periodic transfer method’, but it has the disadvantage of failing to prevent changes in the characteristics of the strain. Changes can arise due to genetic drift or contamination. In the present study, we investigated three categories of method for preserving P. aeruginosa: (a) periodic transfer to fresh media and refrigeration and (b) storage in mineral oil or liquid paraffin and refrigeration and (c) freezing with 15% (v/v) glycerol, followed by retrieval of bacterial culture from frozen stock. Biochemical tests were performed on recovered cultures. P. aeruginosa was found to remain viable for up to 2 months on nutrient agar and Luria-Bertani agar, but just 1 month on brain heart infusion agar and skimmed
milk agar. By contrast, P. aeruginosa suspended in mineral oil or liquid paraffin and frozen with 15% (v/v) glycerol remained viable for over 12 months. Data presented here will help laboratorians make a more informed choice about how they preserve and maintain their bacterial cultures. |
| first_indexed | 2024-04-14T08:12:39Z |
| format | Article |
| id | doaj.art-3b26e87ac47146aba30c19453dd4200c |
| institution | Directory Open Access Journal |
| issn | 1686-9664 |
| language | Thai |
| last_indexed | 2024-04-14T08:12:39Z |
| publishDate | 2019-02-01 |
| publisher | Mahasarakham University |
| record_format | Article |
| series | Warasan Witthayasat Lae Theknoloyi Mahawitthayalai Mahasarakham |
| spelling | doaj.art-3b26e87ac47146aba30c19453dd4200c2022-12-22T02:04:29ZthaMahasarakham UniversityWarasan Witthayasat Lae Theknoloyi Mahawitthayalai Mahasarakham1686-96642019-02-0138117Preservation and maintenance of Pseudomonas aeruginosa for use in the laboratorySujitra Yahom0Faculty of Medicine, Mahasarakham University, Muang District, Maha Sarakham 44000, ThailandThe purpose of preserving and maintaining bacterial cultures in a university setting is to ensure they remain viable for laboratory classes and research, and generate consistent laboratory test results over time. Pseudomonas aeruginosa can be preserved by refrigeration at 4°C, but this is only suitable for short-term storage (2-3 weeks). Longer-term storage can be achieved if the bacteria being stored at 4°C are periodically sub-cultured. This is known as the ‘periodic transfer method’, but it has the disadvantage of failing to prevent changes in the characteristics of the strain. Changes can arise due to genetic drift or contamination. In the present study, we investigated three categories of method for preserving P. aeruginosa: (a) periodic transfer to fresh media and refrigeration and (b) storage in mineral oil or liquid paraffin and refrigeration and (c) freezing with 15% (v/v) glycerol, followed by retrieval of bacterial culture from frozen stock. Biochemical tests were performed on recovered cultures. P. aeruginosa was found to remain viable for up to 2 months on nutrient agar and Luria-Bertani agar, but just 1 month on brain heart infusion agar and skimmed milk agar. By contrast, P. aeruginosa suspended in mineral oil or liquid paraffin and frozen with 15% (v/v) glycerol remained viable for over 12 months. Data presented here will help laboratorians make a more informed choice about how they preserve and maintain their bacterial cultures.http://journal.msu.ac.th/upload/articles/article2427_80695.pdfpreservationmaintenancepseudomonas aeruginosa |
| spellingShingle | Sujitra Yahom Preservation and maintenance of Pseudomonas aeruginosa for use in the laboratory Warasan Witthayasat Lae Theknoloyi Mahawitthayalai Mahasarakham preservation maintenance pseudomonas aeruginosa |
| title | Preservation and maintenance of Pseudomonas aeruginosa for use in the laboratory |
| title_full | Preservation and maintenance of Pseudomonas aeruginosa for use in the laboratory |
| title_fullStr | Preservation and maintenance of Pseudomonas aeruginosa for use in the laboratory |
| title_full_unstemmed | Preservation and maintenance of Pseudomonas aeruginosa for use in the laboratory |
| title_short | Preservation and maintenance of Pseudomonas aeruginosa for use in the laboratory |
| title_sort | preservation and maintenance of pseudomonas aeruginosa for use in the laboratory |
| topic | preservation maintenance pseudomonas aeruginosa |
| url | http://journal.msu.ac.th/upload/articles/article2427_80695.pdf |
| work_keys_str_mv | AT sujitrayahom preservationandmaintenanceofpseudomonasaeruginosaforuseinthelaboratory |