Seed imbibition and germination of Plathymenia reticulata Benth. (Fabaceae) affected by mercury: possible role of aquaporins
ABSTRACTStudies that evaluate the physiological and biochemical mechanisms of germination within forest species are needed in order to improve our understanding of such processes. Mercury and dithiothreitol are indicated as important tools in studies that assess the activity of aquaporins during imb...
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| Format: | Article |
| Language: | English |
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Sociedade Botânica do Brasil
2015-09-01
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| Series: | Acta Botânica Brasílica |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062015000300285&lng=en&tlng=en |
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| author | Amanda Ávila Cardoso Eduardo Euclydes de Lima e Borges Genaina Aparecida de Souza Cristiane Jovelina da Silva Raquel Maria de Oliveira Pires Denise Cunha Fernandes dos Santos Dias |
| author_facet | Amanda Ávila Cardoso Eduardo Euclydes de Lima e Borges Genaina Aparecida de Souza Cristiane Jovelina da Silva Raquel Maria de Oliveira Pires Denise Cunha Fernandes dos Santos Dias |
| author_sort | Amanda Ávila Cardoso |
| collection | DOAJ |
| description | ABSTRACTStudies that evaluate the physiological and biochemical mechanisms of germination within forest species are needed in order to improve our understanding of such processes. Mercury and dithiothreitol are indicated as important tools in studies that assess the activity of aquaporins during imbibition and germination of seeds. To investigate the alterations caused by mercury inPlathymenia reticulata seedsdifferent doses of mercury were used in the presence and absence of dithiothreitol. Mercury had a dose-dependent effect on the seeds; in the most dilute solutions mercury partially inhibited the imbibition process, whereas in the most concentrated solutions it caused the death of the embryos. A delay in the hydration of the seeds may have caused decreased germination as a result of the reduced functionality of the aquaporins that were oxidized by mercury. In the presence of the reducing agent dithiothreitol, the activity of these proteins was restored and the germination process was re-established. These findings indicate the importance of aquaporins in the imbibition and germination stages of P. reticulataseeds, and they provide a better understanding of these important developmental events in plants. |
| first_indexed | 2024-12-13T19:39:34Z |
| format | Article |
| id | doaj.art-3b273ad5038d4ae89b6d52b9653c290c |
| institution | Directory Open Access Journal |
| issn | 1677-941X |
| language | English |
| last_indexed | 2024-12-13T19:39:34Z |
| publishDate | 2015-09-01 |
| publisher | Sociedade Botânica do Brasil |
| record_format | Article |
| series | Acta Botânica Brasílica |
| spelling | doaj.art-3b273ad5038d4ae89b6d52b9653c290c2022-12-21T23:33:44ZengSociedade Botânica do BrasilActa Botânica Brasílica1677-941X2015-09-0129328529110.1590/0102-33062014abb0038S0102-33062015000300285Seed imbibition and germination of Plathymenia reticulata Benth. (Fabaceae) affected by mercury: possible role of aquaporinsAmanda Ávila CardosoEduardo Euclydes de Lima e BorgesGenaina Aparecida de SouzaCristiane Jovelina da SilvaRaquel Maria de Oliveira PiresDenise Cunha Fernandes dos Santos DiasABSTRACTStudies that evaluate the physiological and biochemical mechanisms of germination within forest species are needed in order to improve our understanding of such processes. Mercury and dithiothreitol are indicated as important tools in studies that assess the activity of aquaporins during imbibition and germination of seeds. To investigate the alterations caused by mercury inPlathymenia reticulata seedsdifferent doses of mercury were used in the presence and absence of dithiothreitol. Mercury had a dose-dependent effect on the seeds; in the most dilute solutions mercury partially inhibited the imbibition process, whereas in the most concentrated solutions it caused the death of the embryos. A delay in the hydration of the seeds may have caused decreased germination as a result of the reduced functionality of the aquaporins that were oxidized by mercury. In the presence of the reducing agent dithiothreitol, the activity of these proteins was restored and the germination process was re-established. These findings indicate the importance of aquaporins in the imbibition and germination stages of P. reticulataseeds, and they provide a better understanding of these important developmental events in plants.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062015000300285&lng=en&tlng=enaquaporinsdithiothreitolforest speciesmercuryseed |
| spellingShingle | Amanda Ávila Cardoso Eduardo Euclydes de Lima e Borges Genaina Aparecida de Souza Cristiane Jovelina da Silva Raquel Maria de Oliveira Pires Denise Cunha Fernandes dos Santos Dias Seed imbibition and germination of Plathymenia reticulata Benth. (Fabaceae) affected by mercury: possible role of aquaporins Acta Botânica Brasílica aquaporins dithiothreitol forest species mercury seed |
| title | Seed imbibition and germination of Plathymenia reticulata Benth. (Fabaceae) affected by mercury: possible role of aquaporins |
| title_full | Seed imbibition and germination of Plathymenia reticulata Benth. (Fabaceae) affected by mercury: possible role of aquaporins |
| title_fullStr | Seed imbibition and germination of Plathymenia reticulata Benth. (Fabaceae) affected by mercury: possible role of aquaporins |
| title_full_unstemmed | Seed imbibition and germination of Plathymenia reticulata Benth. (Fabaceae) affected by mercury: possible role of aquaporins |
| title_short | Seed imbibition and germination of Plathymenia reticulata Benth. (Fabaceae) affected by mercury: possible role of aquaporins |
| title_sort | seed imbibition and germination of plathymenia reticulata benth fabaceae affected by mercury possible role of aquaporins |
| topic | aquaporins dithiothreitol forest species mercury seed |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062015000300285&lng=en&tlng=en |
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