A Systemic Study of Subcellular Localization of Porcine Epidemic Diarrhea Virus Proteins
Porcine epidemic diarrhea virus (PEDV), a highly pathogenic enteric coronavirus, is regarded as one of the most severe porcine pathogens. To date, there are still no commercial vaccines or drugs that can provide full protection against the epidemic strains. A better understanding of the subcellular...
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MDPI AG
2022-12-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/11/12/1555 |
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| author | Huixin Zhu Zitong Li Juan Bai Ping Jiang Xianwei Wang Xing Liu |
| author_facet | Huixin Zhu Zitong Li Juan Bai Ping Jiang Xianwei Wang Xing Liu |
| author_sort | Huixin Zhu |
| collection | DOAJ |
| description | Porcine epidemic diarrhea virus (PEDV), a highly pathogenic enteric coronavirus, is regarded as one of the most severe porcine pathogens. To date, there are still no commercial vaccines or drugs that can provide full protection against the epidemic strains. A better understanding of the subcellular location of individual proteins could benefit from studying the protein functions and mechanisms of how the virus regulates key cellular processes, finally leading to the development of antiviral agents. In this study, we characterized the subcellular localization of PEDV proteins using multi-labeled fluorescent immunocytochemistry. As a result, 11 proteins showed cytoplasmic distribution and 10 proteins showed both cytoplasmic and nuclear distribution. Furthermore, we demonstrated that four proteins (Nsp3, Nsp4, Nsp6, and S1) were co-localized in the endoplasmic reticulum (ER), while four proteins (Nsp2, S2, N, and ORF3) were partially observed in the ER, two proteins (E and M) were co-localized in the Golgi apparatus, and two proteins (Nsp2 and E) were partially co-localized with the mitochondria. These viral proteins may perform specific functions at specific cellular locations. Together, these results describe a subcellular localization map of PEDV proteins, which will help to characterize the functions of these proteins in the future. |
| first_indexed | 2024-03-09T15:59:11Z |
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| id | doaj.art-3b28ad10efa24940817e41170a03afd6 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T15:59:11Z |
| publishDate | 2022-12-01 |
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| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-3b28ad10efa24940817e41170a03afd62023-11-24T17:14:44ZengMDPI AGPathogens2076-08172022-12-011112155510.3390/pathogens11121555A Systemic Study of Subcellular Localization of Porcine Epidemic Diarrhea Virus ProteinsHuixin Zhu0Zitong Li1Juan Bai2Ping Jiang3Xianwei Wang4Xing Liu5Key Laboratory of Animal Disease Diagnostics and Immunology, Ministry of Agriculture, MOE International Joint Collaborative Research Laboratory for Animal Health & Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, ChinaKey Laboratory of Animal Disease Diagnostics and Immunology, Ministry of Agriculture, MOE International Joint Collaborative Research Laboratory for Animal Health & Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, ChinaKey Laboratory of Animal Disease Diagnostics and Immunology, Ministry of Agriculture, MOE International Joint Collaborative Research Laboratory for Animal Health & Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, ChinaKey Laboratory of Animal Disease Diagnostics and Immunology, Ministry of Agriculture, MOE International Joint Collaborative Research Laboratory for Animal Health & Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, ChinaKey Laboratory of Animal Disease Diagnostics and Immunology, Ministry of Agriculture, MOE International Joint Collaborative Research Laboratory for Animal Health & Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, ChinaKey Laboratory of Animal Disease Diagnostics and Immunology, Ministry of Agriculture, MOE International Joint Collaborative Research Laboratory for Animal Health & Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, ChinaPorcine epidemic diarrhea virus (PEDV), a highly pathogenic enteric coronavirus, is regarded as one of the most severe porcine pathogens. To date, there are still no commercial vaccines or drugs that can provide full protection against the epidemic strains. A better understanding of the subcellular location of individual proteins could benefit from studying the protein functions and mechanisms of how the virus regulates key cellular processes, finally leading to the development of antiviral agents. In this study, we characterized the subcellular localization of PEDV proteins using multi-labeled fluorescent immunocytochemistry. As a result, 11 proteins showed cytoplasmic distribution and 10 proteins showed both cytoplasmic and nuclear distribution. Furthermore, we demonstrated that four proteins (Nsp3, Nsp4, Nsp6, and S1) were co-localized in the endoplasmic reticulum (ER), while four proteins (Nsp2, S2, N, and ORF3) were partially observed in the ER, two proteins (E and M) were co-localized in the Golgi apparatus, and two proteins (Nsp2 and E) were partially co-localized with the mitochondria. These viral proteins may perform specific functions at specific cellular locations. Together, these results describe a subcellular localization map of PEDV proteins, which will help to characterize the functions of these proteins in the future.https://www.mdpi.com/2076-0817/11/12/1555PEDV proteinssubcellular localizationendoplasmic reticulum (ER)Golgi apparatusmitochondria |
| spellingShingle | Huixin Zhu Zitong Li Juan Bai Ping Jiang Xianwei Wang Xing Liu A Systemic Study of Subcellular Localization of Porcine Epidemic Diarrhea Virus Proteins Pathogens PEDV proteins subcellular localization endoplasmic reticulum (ER) Golgi apparatus mitochondria |
| title | A Systemic Study of Subcellular Localization of Porcine Epidemic Diarrhea Virus Proteins |
| title_full | A Systemic Study of Subcellular Localization of Porcine Epidemic Diarrhea Virus Proteins |
| title_fullStr | A Systemic Study of Subcellular Localization of Porcine Epidemic Diarrhea Virus Proteins |
| title_full_unstemmed | A Systemic Study of Subcellular Localization of Porcine Epidemic Diarrhea Virus Proteins |
| title_short | A Systemic Study of Subcellular Localization of Porcine Epidemic Diarrhea Virus Proteins |
| title_sort | systemic study of subcellular localization of porcine epidemic diarrhea virus proteins |
| topic | PEDV proteins subcellular localization endoplasmic reticulum (ER) Golgi apparatus mitochondria |
| url | https://www.mdpi.com/2076-0817/11/12/1555 |
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