Genotoxicity of PM<sub>2.5</sub> and PM<sub>1.0</sub> Particulates on Human Peripheral Blood Lymphocytes in Manila, Philippines
Urban air quality is increasingly being studied as a fraction of the world’s population is living in megacities. In this study, particulate matter (PM) along Taft Avenue, Manila, the Philippines, is investigated in terms of its ability to induce genetic damage in human peripheral blood lymphocytes (...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-12-01
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| Series: | Atmosphere |
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| Online Access: | https://www.mdpi.com/2073-4433/13/1/6 |
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| author | Ma. Katrina Gale Estonilo Joedith Anne Cazeñas Carlos Josef Villafuerte Custer Deocaris Gloriamaris Caraos Gerardo Jose Robles Maria Cecilia Galvez Celia Asaad Edgar Vallar |
| author_facet | Ma. Katrina Gale Estonilo Joedith Anne Cazeñas Carlos Josef Villafuerte Custer Deocaris Gloriamaris Caraos Gerardo Jose Robles Maria Cecilia Galvez Celia Asaad Edgar Vallar |
| author_sort | Ma. Katrina Gale Estonilo |
| collection | DOAJ |
| description | Urban air quality is increasingly being studied as a fraction of the world’s population is living in megacities. In this study, particulate matter (PM) along Taft Avenue, Manila, the Philippines, is investigated in terms of its ability to induce genetic damage in human peripheral blood lymphocytes (PBLs). Size-segregated roadside air samples were obtained from 2015–2017 near a university gate and analyzed using in vitro micronucleus (MN) and cytokinesis-block proliferation tests. While cellular proliferation was unaffected by 0–0.1 kg/m<sup>3</sup> of PM<sub>1.0</sub> and PM<sub>2.5</sub>, PBL cells treated with PM<sub>2.5</sub> displayed a significantly higher micronucleus count (<i>p</i> = 0.03) compared to the cells treated with PM<sub>1.0</sub>. Atomic absorption spectroscopy revealed greater amounts of Cd, Ca, Pb, K, Na, and Zn in PM<sub>2.5</sub> compared to PM<sub>1.0</sub>. The results indicate that the differences in composition of the two size fractions of air particulates are associated with their genotoxicities. |
| first_indexed | 2024-03-10T01:56:42Z |
| format | Article |
| id | doaj.art-3b28f25dba094c8ba4f03011ef6c486d |
| institution | Directory Open Access Journal |
| issn | 2073-4433 |
| language | English |
| last_indexed | 2024-03-10T01:56:42Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Atmosphere |
| spelling | doaj.art-3b28f25dba094c8ba4f03011ef6c486d2023-11-23T12:55:34ZengMDPI AGAtmosphere2073-44332021-12-01131610.3390/atmos13010006Genotoxicity of PM<sub>2.5</sub> and PM<sub>1.0</sub> Particulates on Human Peripheral Blood Lymphocytes in Manila, PhilippinesMa. Katrina Gale Estonilo0Joedith Anne Cazeñas1Carlos Josef Villafuerte2Custer Deocaris3Gloriamaris Caraos4Gerardo Jose Robles5Maria Cecilia Galvez6Celia Asaad7Edgar Vallar8Environment and Remote Sensing Research (EARTH) Laboratory, Department of Physics, De La Salle University, Manila 0922, PhilippinesEnvironment and Remote Sensing Research (EARTH) Laboratory, Department of Physics, De La Salle University, Manila 0922, PhilippinesEnvironment and Remote Sensing Research (EARTH) Laboratory, Department of Physics, De La Salle University, Manila 0922, PhilippinesBiomedical Research Unit, Atomic Research Division, Philippine Nuclear Research Institute, Department of Science and Technology, Diliman, Quezon City 1101, PhilippinesBiomedical Research Unit, Atomic Research Division, Philippine Nuclear Research Institute, Department of Science and Technology, Diliman, Quezon City 1101, PhilippinesBiomedical Research Unit, Atomic Research Division, Philippine Nuclear Research Institute, Department of Science and Technology, Diliman, Quezon City 1101, PhilippinesEnvironment and Remote Sensing Research (EARTH) Laboratory, Department of Physics, De La Salle University, Manila 0922, PhilippinesBiomedical Research Unit, Atomic Research Division, Philippine Nuclear Research Institute, Department of Science and Technology, Diliman, Quezon City 1101, PhilippinesEnvironment and Remote Sensing Research (EARTH) Laboratory, Department of Physics, De La Salle University, Manila 0922, PhilippinesUrban air quality is increasingly being studied as a fraction of the world’s population is living in megacities. In this study, particulate matter (PM) along Taft Avenue, Manila, the Philippines, is investigated in terms of its ability to induce genetic damage in human peripheral blood lymphocytes (PBLs). Size-segregated roadside air samples were obtained from 2015–2017 near a university gate and analyzed using in vitro micronucleus (MN) and cytokinesis-block proliferation tests. While cellular proliferation was unaffected by 0–0.1 kg/m<sup>3</sup> of PM<sub>1.0</sub> and PM<sub>2.5</sub>, PBL cells treated with PM<sub>2.5</sub> displayed a significantly higher micronucleus count (<i>p</i> = 0.03) compared to the cells treated with PM<sub>1.0</sub>. Atomic absorption spectroscopy revealed greater amounts of Cd, Ca, Pb, K, Na, and Zn in PM<sub>2.5</sub> compared to PM<sub>1.0</sub>. The results indicate that the differences in composition of the two size fractions of air particulates are associated with their genotoxicities.https://www.mdpi.com/2073-4433/13/1/6particulate mattercytokinesis-block proliferation indexair pollutionatomic absorption spectroscopyin vitro micronucleus test |
| spellingShingle | Ma. Katrina Gale Estonilo Joedith Anne Cazeñas Carlos Josef Villafuerte Custer Deocaris Gloriamaris Caraos Gerardo Jose Robles Maria Cecilia Galvez Celia Asaad Edgar Vallar Genotoxicity of PM<sub>2.5</sub> and PM<sub>1.0</sub> Particulates on Human Peripheral Blood Lymphocytes in Manila, Philippines Atmosphere particulate matter cytokinesis-block proliferation index air pollution atomic absorption spectroscopy in vitro micronucleus test |
| title | Genotoxicity of PM<sub>2.5</sub> and PM<sub>1.0</sub> Particulates on Human Peripheral Blood Lymphocytes in Manila, Philippines |
| title_full | Genotoxicity of PM<sub>2.5</sub> and PM<sub>1.0</sub> Particulates on Human Peripheral Blood Lymphocytes in Manila, Philippines |
| title_fullStr | Genotoxicity of PM<sub>2.5</sub> and PM<sub>1.0</sub> Particulates on Human Peripheral Blood Lymphocytes in Manila, Philippines |
| title_full_unstemmed | Genotoxicity of PM<sub>2.5</sub> and PM<sub>1.0</sub> Particulates on Human Peripheral Blood Lymphocytes in Manila, Philippines |
| title_short | Genotoxicity of PM<sub>2.5</sub> and PM<sub>1.0</sub> Particulates on Human Peripheral Blood Lymphocytes in Manila, Philippines |
| title_sort | genotoxicity of pm sub 2 5 sub and pm sub 1 0 sub particulates on human peripheral blood lymphocytes in manila philippines |
| topic | particulate matter cytokinesis-block proliferation index air pollution atomic absorption spectroscopy in vitro micronucleus test |
| url | https://www.mdpi.com/2073-4433/13/1/6 |
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