Surface and Extracellular Proteome of the Emerging Pathogen Corynebacterium ulcerans
Corynebacterium ulcerans is an emerging pathogen, which is increasingly recognized as an etiological agent of diphtheria, but can also evoke ulcers of the skin and systemic infections in humans. Besides man, the bacteria can colonize a wide variety of different animals, including cattle and pet anim...
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-04-01
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| Series: | Proteomes |
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| Online Access: | http://www.mdpi.com/2227-7382/6/2/18 |
| _version_ | 1818000357428559872 |
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| author | Miriam Bittel Susanne Gastiger Bushra Amin Jörg Hofmann Andreas Burkovski |
| author_facet | Miriam Bittel Susanne Gastiger Bushra Amin Jörg Hofmann Andreas Burkovski |
| author_sort | Miriam Bittel |
| collection | DOAJ |
| description | Corynebacterium ulcerans is an emerging pathogen, which is increasingly recognized as an etiological agent of diphtheria, but can also evoke ulcers of the skin and systemic infections in humans. Besides man, the bacteria can colonize a wide variety of different animals, including cattle and pet animals, which might serve as a reservoir for human infections. In this study, surface-located proteins and the exoproteome of two Corynebacterium ulcerans strains were analyzed, since these may have key roles in the interaction of the pathogen with host cells. Strain 809 was isolated from a fatal case of human respiratory tract infection, while strain BR-AD22 was isolated from a nasal swap of an asymptomatic dog. While a very similar pattern of virulence factors was observed in the culture supernatant and surface protein fractions of the two strains, proteome analyses revealed a higher stability of 809 cells compared to strain BR-AD22. During exponential growth, 17% of encoded proteins of strain 809 were detectable in the medium, while 38% of the predicted proteins encoded by the BR-AD22 chromosome were found. Furthermore, the data indicate differential expression of phospholipase D and a cell wall-associated hydrolase, since these were only detected in strain BR-AD22. |
| first_indexed | 2024-04-14T03:20:38Z |
| format | Article |
| id | doaj.art-3b2c36e818a74498a26acf01a29842b2 |
| institution | Directory Open Access Journal |
| issn | 2227-7382 |
| language | English |
| last_indexed | 2024-04-14T03:20:38Z |
| publishDate | 2018-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Proteomes |
| spelling | doaj.art-3b2c36e818a74498a26acf01a29842b22022-12-22T02:15:19ZengMDPI AGProteomes2227-73822018-04-01621810.3390/proteomes6020018proteomes6020018Surface and Extracellular Proteome of the Emerging Pathogen Corynebacterium ulceransMiriam Bittel0Susanne Gastiger1Bushra Amin2Jörg Hofmann3Andreas Burkovski4Professur für Mikrobiologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Staudtstr. 5, 91058 Erlangen, GermanyProfessur für Mikrobiologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Staudtstr. 5, 91058 Erlangen, GermanyLehrstuhl für Biochemie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Staudtstr. 5, 91058 Erlangen, GermanyLehrstuhl für Biochemie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Staudtstr. 5, 91058 Erlangen, GermanyProfessur für Mikrobiologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Staudtstr. 5, 91058 Erlangen, GermanyCorynebacterium ulcerans is an emerging pathogen, which is increasingly recognized as an etiological agent of diphtheria, but can also evoke ulcers of the skin and systemic infections in humans. Besides man, the bacteria can colonize a wide variety of different animals, including cattle and pet animals, which might serve as a reservoir for human infections. In this study, surface-located proteins and the exoproteome of two Corynebacterium ulcerans strains were analyzed, since these may have key roles in the interaction of the pathogen with host cells. Strain 809 was isolated from a fatal case of human respiratory tract infection, while strain BR-AD22 was isolated from a nasal swap of an asymptomatic dog. While a very similar pattern of virulence factors was observed in the culture supernatant and surface protein fractions of the two strains, proteome analyses revealed a higher stability of 809 cells compared to strain BR-AD22. During exponential growth, 17% of encoded proteins of strain 809 were detectable in the medium, while 38% of the predicted proteins encoded by the BR-AD22 chromosome were found. Furthermore, the data indicate differential expression of phospholipase D and a cell wall-associated hydrolase, since these were only detected in strain BR-AD22.http://www.mdpi.com/2227-7382/6/2/18diphtheriaexoproteomegenomicshost-pathogen interactionsurface proteomevirulence factors |
| spellingShingle | Miriam Bittel Susanne Gastiger Bushra Amin Jörg Hofmann Andreas Burkovski Surface and Extracellular Proteome of the Emerging Pathogen Corynebacterium ulcerans Proteomes diphtheria exoproteome genomics host-pathogen interaction surface proteome virulence factors |
| title | Surface and Extracellular Proteome of the Emerging Pathogen Corynebacterium ulcerans |
| title_full | Surface and Extracellular Proteome of the Emerging Pathogen Corynebacterium ulcerans |
| title_fullStr | Surface and Extracellular Proteome of the Emerging Pathogen Corynebacterium ulcerans |
| title_full_unstemmed | Surface and Extracellular Proteome of the Emerging Pathogen Corynebacterium ulcerans |
| title_short | Surface and Extracellular Proteome of the Emerging Pathogen Corynebacterium ulcerans |
| title_sort | surface and extracellular proteome of the emerging pathogen corynebacterium ulcerans |
| topic | diphtheria exoproteome genomics host-pathogen interaction surface proteome virulence factors |
| url | http://www.mdpi.com/2227-7382/6/2/18 |
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