Occurrence and Function of the Na<sup>+</sup>-Translocating NADH:Quinone Oxidoreductase in <i>Prevotella</i> spp.
Strictly anaerobic <i>Prevotella</i> spp. are characterized by their vast metabolic potential. As members of the Prevotellaceae family, they represent the most abundant organisms in the rumen and are typically found in monogastrics such as pigs and humans. Within their largely anoxic hab...
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MDPI AG
2019-04-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/7/5/117 |
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| author | Simon Deusch Eva Bok Lena Schleicher Jana Seifert Julia Steuber |
| author_facet | Simon Deusch Eva Bok Lena Schleicher Jana Seifert Julia Steuber |
| author_sort | Simon Deusch |
| collection | DOAJ |
| description | Strictly anaerobic <i>Prevotella</i> spp. are characterized by their vast metabolic potential. As members of the Prevotellaceae family, they represent the most abundant organisms in the rumen and are typically found in monogastrics such as pigs and humans. Within their largely anoxic habitats, these bacteria are considered to rely primarily on fermentation for energy conservation. A recent study of the rumen microbiome identified multiple subunits of the Na<sup>+</sup>-translocating NADH:quinone oxidoreductase (NQR) belonging to different <i>Prevotella</i> spp. Commonly, the NQR is associated with biochemical energy generation by respiration. The existence of this Na<sup>+</sup> pump in <i>Prevotella</i> spp. may indicate an important role for electrochemical Na<sup>+</sup> gradients in their anaerobic metabolism. However, detailed information about the potential activity of the NQR in <i>Prevotella</i> spp. is not available. Here, the presence of a functioning NQR in the strictly anaerobic model organism <i>P. bryantii</i> B<sub>1</sub>4 was verified by conducting mass spectrometric, biochemical, and kinetic experiments. Our findings propose that <i>P. bryantii</i> B<sub>1</sub>4 and other <i>Prevotella</i> spp. retrieved from the rumen operate a respiratory NQR together with a fumarate reductase which suggests that these ruminal bacteria utilize a sodium motive force generated during respiratory NADH:fumarate oxidoreduction. |
| first_indexed | 2024-12-22T13:38:09Z |
| format | Article |
| id | doaj.art-3b2c7e3a183e472dbe2066a51444c396 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-12-22T13:38:09Z |
| publishDate | 2019-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-3b2c7e3a183e472dbe2066a51444c3962022-12-21T18:23:59ZengMDPI AGMicroorganisms2076-26072019-04-017511710.3390/microorganisms7050117microorganisms7050117Occurrence and Function of the Na<sup>+</sup>-Translocating NADH:Quinone Oxidoreductase in <i>Prevotella</i> spp.Simon Deusch0Eva Bok1Lena Schleicher2Jana Seifert3Julia Steuber4Institute of Animal Science, University of Hohenheim, 70599 Stuttgart, GermanyInstitute of Microbiology, University of Hohenheim, 70599 Stuttgart, GermanyInstitute of Microbiology, University of Hohenheim, 70599 Stuttgart, GermanyInstitute of Animal Science, University of Hohenheim, 70599 Stuttgart, GermanyInstitute of Microbiology, University of Hohenheim, 70599 Stuttgart, GermanyStrictly anaerobic <i>Prevotella</i> spp. are characterized by their vast metabolic potential. As members of the Prevotellaceae family, they represent the most abundant organisms in the rumen and are typically found in monogastrics such as pigs and humans. Within their largely anoxic habitats, these bacteria are considered to rely primarily on fermentation for energy conservation. A recent study of the rumen microbiome identified multiple subunits of the Na<sup>+</sup>-translocating NADH:quinone oxidoreductase (NQR) belonging to different <i>Prevotella</i> spp. Commonly, the NQR is associated with biochemical energy generation by respiration. The existence of this Na<sup>+</sup> pump in <i>Prevotella</i> spp. may indicate an important role for electrochemical Na<sup>+</sup> gradients in their anaerobic metabolism. However, detailed information about the potential activity of the NQR in <i>Prevotella</i> spp. is not available. Here, the presence of a functioning NQR in the strictly anaerobic model organism <i>P. bryantii</i> B<sub>1</sub>4 was verified by conducting mass spectrometric, biochemical, and kinetic experiments. Our findings propose that <i>P. bryantii</i> B<sub>1</sub>4 and other <i>Prevotella</i> spp. retrieved from the rumen operate a respiratory NQR together with a fumarate reductase which suggests that these ruminal bacteria utilize a sodium motive force generated during respiratory NADH:fumarate oxidoreduction.https://www.mdpi.com/2076-2607/7/5/117PrevotellaceaeNQR<i>P. bryantii</i> B<sub>1</sub>4proteomics |
| spellingShingle | Simon Deusch Eva Bok Lena Schleicher Jana Seifert Julia Steuber Occurrence and Function of the Na<sup>+</sup>-Translocating NADH:Quinone Oxidoreductase in <i>Prevotella</i> spp. Microorganisms Prevotellaceae NQR <i>P. bryantii</i> B<sub>1</sub>4 proteomics |
| title | Occurrence and Function of the Na<sup>+</sup>-Translocating NADH:Quinone Oxidoreductase in <i>Prevotella</i> spp. |
| title_full | Occurrence and Function of the Na<sup>+</sup>-Translocating NADH:Quinone Oxidoreductase in <i>Prevotella</i> spp. |
| title_fullStr | Occurrence and Function of the Na<sup>+</sup>-Translocating NADH:Quinone Oxidoreductase in <i>Prevotella</i> spp. |
| title_full_unstemmed | Occurrence and Function of the Na<sup>+</sup>-Translocating NADH:Quinone Oxidoreductase in <i>Prevotella</i> spp. |
| title_short | Occurrence and Function of the Na<sup>+</sup>-Translocating NADH:Quinone Oxidoreductase in <i>Prevotella</i> spp. |
| title_sort | occurrence and function of the na sup sup translocating nadh quinone oxidoreductase in i prevotella i spp |
| topic | Prevotellaceae NQR <i>P. bryantii</i> B<sub>1</sub>4 proteomics |
| url | https://www.mdpi.com/2076-2607/7/5/117 |
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