Characterising the KMP-11 and HSP-70 recombinant antigens' humoral immune response profile in chagasic patients
<p>Abstract</p> <p>Background</p> <p>Antigen specificity and IgG subclass could be significant in the natural history of Chagas' disease. The relationship between the different stages of human Chagas' disease and the profiles of total IgG and its subclasses we...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2009-01-01
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| Series: | BMC Infectious Diseases |
| Online Access: | http://www.biomedcentral.com/1471-2334/9/186 |
| _version_ | 1818662253813039104 |
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| author | Steindel Mario Cuellar Adriana González John Rosas Fernando Velasco Víctor Cucunubá Zulma Thomas María López Manuel Flechas Ivonne Puerta Concepción |
| author_facet | Steindel Mario Cuellar Adriana González John Rosas Fernando Velasco Víctor Cucunubá Zulma Thomas María López Manuel Flechas Ivonne Puerta Concepción |
| author_sort | Steindel Mario |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Antigen specificity and IgG subclass could be significant in the natural history of Chagas' disease. The relationship between the different stages of human Chagas' disease and the profiles of total IgG and its subclasses were thus analysed here; they were directed against a crude <it>T. cruzi </it>extract and three recombinant antigens: the <it>T. cruzi </it>kinetoplastid membrane protein-11 (rKMP-11), an internal fragment of the <it>T. cruzi </it>HSP-70 protein<sub>192-433</sub>, and the entire <it>Trypanosoma rangeli </it>HSP-70 protein.</p> <p>Methods</p> <p>Seventeen Brazilian acute chagasic patients, 50 Colombian chronic chagasic patients (21 indeterminate and 29 cardiopathic patients) and 30 healthy individuals were included. Total IgG and its subtypes directed against the above-mentioned recombinant antigens were determined by ELISA tests.</p> <p>Results</p> <p>The <it>T. cruzi </it>KMP-11 and <it>T. rangeli </it>HSP-70 recombinant proteins were able to distinguish both acute from chronic chagasic patients and infected people from healthy individuals. Specific antibodies to <it>T. cruzi </it>crude antigen in acute patients came from IgG3 and IgG4 subclasses whereas IgG1 and IgG3 were the prevalent isotypes in indeterminate and chronic chagasic patients. By contrast, the specific prominent antibodies in all disease stages against <it>T. cruzi </it>KMP-11 and <it>T. rangeli </it>HSP-70 recombinant antigens were the IgG1 subclass.</p> <p>Conclusion</p> <p><it>T. cruzi </it>KMP-11 and the <it>T. rangeli </it>HSP-70 recombinant proteins may be explored together in the immunodiagnosis of Chagas' disease.</p> <p>Polarising the IgG1 subclass of the IgG response to <it>T. cruzi </it>KMP-11 and <it>T. rangeli </it>HSP-70 recombinant proteins could have important biological effects, taking into account that this is a complement fixing antibody.</p> |
| first_indexed | 2024-12-17T04:58:01Z |
| format | Article |
| id | doaj.art-3b2f7080c4834cd5be185a9a1090f7b7 |
| institution | Directory Open Access Journal |
| issn | 1471-2334 |
| language | English |
| last_indexed | 2024-12-17T04:58:01Z |
| publishDate | 2009-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Infectious Diseases |
| spelling | doaj.art-3b2f7080c4834cd5be185a9a1090f7b72022-12-21T22:02:39ZengBMCBMC Infectious Diseases1471-23342009-01-0191186Characterising the KMP-11 and HSP-70 recombinant antigens' humoral immune response profile in chagasic patientsSteindel MarioCuellar AdrianaGonzález JohnRosas FernandoVelasco VíctorCucunubá ZulmaThomas MaríaLópez ManuelFlechas IvonnePuerta Concepción<p>Abstract</p> <p>Background</p> <p>Antigen specificity and IgG subclass could be significant in the natural history of Chagas' disease. The relationship between the different stages of human Chagas' disease and the profiles of total IgG and its subclasses were thus analysed here; they were directed against a crude <it>T. cruzi </it>extract and three recombinant antigens: the <it>T. cruzi </it>kinetoplastid membrane protein-11 (rKMP-11), an internal fragment of the <it>T. cruzi </it>HSP-70 protein<sub>192-433</sub>, and the entire <it>Trypanosoma rangeli </it>HSP-70 protein.</p> <p>Methods</p> <p>Seventeen Brazilian acute chagasic patients, 50 Colombian chronic chagasic patients (21 indeterminate and 29 cardiopathic patients) and 30 healthy individuals were included. Total IgG and its subtypes directed against the above-mentioned recombinant antigens were determined by ELISA tests.</p> <p>Results</p> <p>The <it>T. cruzi </it>KMP-11 and <it>T. rangeli </it>HSP-70 recombinant proteins were able to distinguish both acute from chronic chagasic patients and infected people from healthy individuals. Specific antibodies to <it>T. cruzi </it>crude antigen in acute patients came from IgG3 and IgG4 subclasses whereas IgG1 and IgG3 were the prevalent isotypes in indeterminate and chronic chagasic patients. By contrast, the specific prominent antibodies in all disease stages against <it>T. cruzi </it>KMP-11 and <it>T. rangeli </it>HSP-70 recombinant antigens were the IgG1 subclass.</p> <p>Conclusion</p> <p><it>T. cruzi </it>KMP-11 and the <it>T. rangeli </it>HSP-70 recombinant proteins may be explored together in the immunodiagnosis of Chagas' disease.</p> <p>Polarising the IgG1 subclass of the IgG response to <it>T. cruzi </it>KMP-11 and <it>T. rangeli </it>HSP-70 recombinant proteins could have important biological effects, taking into account that this is a complement fixing antibody.</p>http://www.biomedcentral.com/1471-2334/9/186 |
| spellingShingle | Steindel Mario Cuellar Adriana González John Rosas Fernando Velasco Víctor Cucunubá Zulma Thomas María López Manuel Flechas Ivonne Puerta Concepción Characterising the KMP-11 and HSP-70 recombinant antigens' humoral immune response profile in chagasic patients BMC Infectious Diseases |
| title | Characterising the KMP-11 and HSP-70 recombinant antigens' humoral immune response profile in chagasic patients |
| title_full | Characterising the KMP-11 and HSP-70 recombinant antigens' humoral immune response profile in chagasic patients |
| title_fullStr | Characterising the KMP-11 and HSP-70 recombinant antigens' humoral immune response profile in chagasic patients |
| title_full_unstemmed | Characterising the KMP-11 and HSP-70 recombinant antigens' humoral immune response profile in chagasic patients |
| title_short | Characterising the KMP-11 and HSP-70 recombinant antigens' humoral immune response profile in chagasic patients |
| title_sort | characterising the kmp 11 and hsp 70 recombinant antigens humoral immune response profile in chagasic patients |
| url | http://www.biomedcentral.com/1471-2334/9/186 |
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