Detection of human pathogenic bacteria in rectal DNA samples from Zalophus californianus in the Gulf of California, Mexico
Abstract Human intrusions into undisturbed wildlife areas greatly contribute to the emergence of infectious diseases. To minimize the impacts of novel emerging infectious diseases (EIDs) on human health, a comprehensive understanding of the microbial species that reside within wildlife species is re...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-09-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-18903-4 |
| _version_ | 1798032542662656000 |
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| author | Francesco Cicala David Ramírez-Delgado Ricardo Gómez-Reyes Marcel Martínez-Porchas Jorge Rojas-Vargas Liliana Pardo-López Alexei F. Licea-Navarro |
| author_facet | Francesco Cicala David Ramírez-Delgado Ricardo Gómez-Reyes Marcel Martínez-Porchas Jorge Rojas-Vargas Liliana Pardo-López Alexei F. Licea-Navarro |
| author_sort | Francesco Cicala |
| collection | DOAJ |
| description | Abstract Human intrusions into undisturbed wildlife areas greatly contribute to the emergence of infectious diseases. To minimize the impacts of novel emerging infectious diseases (EIDs) on human health, a comprehensive understanding of the microbial species that reside within wildlife species is required. The Gulf of California (GoC) is an example of an undisturbed ecosystem. However, in recent decades, anthropogenic activities within the GoC have increased. Zalophus californianus has been proposed as the main sentinel species in the GoC; hence, an assessment of sea lion bacterial microbiota may reveal hidden risks for human health. We evaluated the presence of potential human pathogenic bacterial species from the gastrointestinal (GI) tracts of wild sea lions through a metabarcoding approach. To comprehensively evaluate this bacterial consortium, we considered the genetic information of six hypervariable regions of 16S rRNA. Potential human pathogenic bacteria were identified down to the species level by integrating the RDP and Pplacer classifier outputs. The combined genetic information from all analyzed regions suggests the presence of at least 44 human pathogenic bacterial species, including Shigella dysenteriae and Bacillus anthracis. Therefore, the risks of EIDs from this area should be not underestimated. |
| first_indexed | 2024-04-11T20:15:43Z |
| format | Article |
| id | doaj.art-3b2f82f42ca34e40b50f9dd35eda7de6 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-11T20:15:43Z |
| publishDate | 2022-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-3b2f82f42ca34e40b50f9dd35eda7de62022-12-22T04:04:59ZengNature PortfolioScientific Reports2045-23222022-09-0112111010.1038/s41598-022-18903-4Detection of human pathogenic bacteria in rectal DNA samples from Zalophus californianus in the Gulf of California, MexicoFrancesco Cicala0David Ramírez-Delgado1Ricardo Gómez-Reyes2Marcel Martínez-Porchas3Jorge Rojas-Vargas4Liliana Pardo-López5Alexei F. Licea-Navarro6Department of Biomedical Innovation, Ensenada Center for Scientific Research and Higher EducationDepartment of Marine Ecology, Ensenada Center for Scientific Research and Higher EducationOceanology Research Institute. Autonomous University of Baja CaliforniaLaboratory of Experimental Biology, Center for Research in Food and Development, A.C.Department of Molecular Microbiology, Institute of Biotechnology, National Autonomous University of MexicoDepartment of Molecular Microbiology, Institute of Biotechnology, National Autonomous University of MexicoDepartment of Biomedical Innovation, Ensenada Center for Scientific Research and Higher EducationAbstract Human intrusions into undisturbed wildlife areas greatly contribute to the emergence of infectious diseases. To minimize the impacts of novel emerging infectious diseases (EIDs) on human health, a comprehensive understanding of the microbial species that reside within wildlife species is required. The Gulf of California (GoC) is an example of an undisturbed ecosystem. However, in recent decades, anthropogenic activities within the GoC have increased. Zalophus californianus has been proposed as the main sentinel species in the GoC; hence, an assessment of sea lion bacterial microbiota may reveal hidden risks for human health. We evaluated the presence of potential human pathogenic bacterial species from the gastrointestinal (GI) tracts of wild sea lions through a metabarcoding approach. To comprehensively evaluate this bacterial consortium, we considered the genetic information of six hypervariable regions of 16S rRNA. Potential human pathogenic bacteria were identified down to the species level by integrating the RDP and Pplacer classifier outputs. The combined genetic information from all analyzed regions suggests the presence of at least 44 human pathogenic bacterial species, including Shigella dysenteriae and Bacillus anthracis. Therefore, the risks of EIDs from this area should be not underestimated.https://doi.org/10.1038/s41598-022-18903-4 |
| spellingShingle | Francesco Cicala David Ramírez-Delgado Ricardo Gómez-Reyes Marcel Martínez-Porchas Jorge Rojas-Vargas Liliana Pardo-López Alexei F. Licea-Navarro Detection of human pathogenic bacteria in rectal DNA samples from Zalophus californianus in the Gulf of California, Mexico Scientific Reports |
| title | Detection of human pathogenic bacteria in rectal DNA samples from Zalophus californianus in the Gulf of California, Mexico |
| title_full | Detection of human pathogenic bacteria in rectal DNA samples from Zalophus californianus in the Gulf of California, Mexico |
| title_fullStr | Detection of human pathogenic bacteria in rectal DNA samples from Zalophus californianus in the Gulf of California, Mexico |
| title_full_unstemmed | Detection of human pathogenic bacteria in rectal DNA samples from Zalophus californianus in the Gulf of California, Mexico |
| title_short | Detection of human pathogenic bacteria in rectal DNA samples from Zalophus californianus in the Gulf of California, Mexico |
| title_sort | detection of human pathogenic bacteria in rectal dna samples from zalophus californianus in the gulf of california mexico |
| url | https://doi.org/10.1038/s41598-022-18903-4 |
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