Heat Stress Directly Affects Intestinal Integrity in Broiler Chickens
A study using pair-feeding technique was conducted to determine whether heat exposure directly or indirectly (via reduced feed intake) increases intestinal mucosal damage and permeability to endotoxin in broiler chickens. Male broiler chickens (Ross 308), 27-d-old, were subjected to one of the three...
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| Format: | Article |
| Language: | English |
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Japan Poultry Science Association
2020-10-01
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| Series: | The Journal of Poultry Science |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jpsa/57/4/57_0190004/_html/-char/en |
| _version_ | 1797817055753273344 |
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| author | Fumika Nanto-Hara Motoi Kikusato Shyuichi Ohwada Masaaki Toyomizu |
| author_facet | Fumika Nanto-Hara Motoi Kikusato Shyuichi Ohwada Masaaki Toyomizu |
| author_sort | Fumika Nanto-Hara |
| collection | DOAJ |
| description | A study using pair-feeding technique was conducted to determine whether heat exposure directly or indirectly (via reduced feed intake) increases intestinal mucosal damage and permeability to endotoxin in broiler chickens. Male broiler chickens (Ross 308), 27-d-old, were subjected to one of the three treatments (n=8): 1) thermo-neutral conditions (24°C) with ad libitum feed intake, 2) heat stress conditions (33°C) with ad libitum feed intake, or 3) pair-feeding under thermo-neutral conditions, with the feed intake identical to that of heat-stressed chickens. Using these groups, two experiments were performed to evaluate temporal changes in the intestinal morphology in response to each treatment. In experiment 1, chickens were sacrificed after 24 h of exposure to the treatment conditions, while in experiment 2, chickens were sacrificed after 12 or 72 h of exposure to the treatment conditions. In experiment 1, exposure to heat stress conditions for 24 h significantly decreased both the villus height to crypt depth ratio and number of proliferating cell nuclear antigen (PCNA)-positive cells in the duodenum and increased the plasma endotoxin concentration. These findings were not observed in pair-fed chickens. In experiment 2, intestinal integrity and function were unaffected by 12 h of heat stress. On the other hand, chickens exposed to heat stress for 72 h exhibited significantly damaged intestinal morphology in the duodenum as well as increased plasma endotoxin concentration; these negative effects were not observed in pair-fed chickens. These findings suggest that the intestinal morphology and permeability changes observed in chickens that are heat-stressed for 24–72 h are due to the heat stress conditions and not due to reduced feed intake. |
| first_indexed | 2024-03-13T08:46:51Z |
| format | Article |
| id | doaj.art-3b3a80d816db4622b41507bec74c5dd6 |
| institution | Directory Open Access Journal |
| issn | 1346-7395 1349-0486 |
| language | English |
| last_indexed | 2024-03-13T08:46:51Z |
| publishDate | 2020-10-01 |
| publisher | Japan Poultry Science Association |
| record_format | Article |
| series | The Journal of Poultry Science |
| spelling | doaj.art-3b3a80d816db4622b41507bec74c5dd62023-05-30T01:08:01ZengJapan Poultry Science AssociationThe Journal of Poultry Science1346-73951349-04862020-10-0157428429010.2141/jpsa.0190004jpsaHeat Stress Directly Affects Intestinal Integrity in Broiler ChickensFumika Nanto-Hara0Motoi Kikusato1Shyuichi Ohwada2Masaaki Toyomizu3Animal Nutrition, Graduate School of Agricultural Science, Tohoku UniversityAnimal Nutrition, Graduate School of Agricultural Science, Tohoku UniversityAnimal Nutrition, Graduate School of Agricultural Science, Tohoku UniversityAnimal Nutrition, Graduate School of Agricultural Science, Tohoku UniversityA study using pair-feeding technique was conducted to determine whether heat exposure directly or indirectly (via reduced feed intake) increases intestinal mucosal damage and permeability to endotoxin in broiler chickens. Male broiler chickens (Ross 308), 27-d-old, were subjected to one of the three treatments (n=8): 1) thermo-neutral conditions (24°C) with ad libitum feed intake, 2) heat stress conditions (33°C) with ad libitum feed intake, or 3) pair-feeding under thermo-neutral conditions, with the feed intake identical to that of heat-stressed chickens. Using these groups, two experiments were performed to evaluate temporal changes in the intestinal morphology in response to each treatment. In experiment 1, chickens were sacrificed after 24 h of exposure to the treatment conditions, while in experiment 2, chickens were sacrificed after 12 or 72 h of exposure to the treatment conditions. In experiment 1, exposure to heat stress conditions for 24 h significantly decreased both the villus height to crypt depth ratio and number of proliferating cell nuclear antigen (PCNA)-positive cells in the duodenum and increased the plasma endotoxin concentration. These findings were not observed in pair-fed chickens. In experiment 2, intestinal integrity and function were unaffected by 12 h of heat stress. On the other hand, chickens exposed to heat stress for 72 h exhibited significantly damaged intestinal morphology in the duodenum as well as increased plasma endotoxin concentration; these negative effects were not observed in pair-fed chickens. These findings suggest that the intestinal morphology and permeability changes observed in chickens that are heat-stressed for 24–72 h are due to the heat stress conditions and not due to reduced feed intake.https://www.jstage.jst.go.jp/article/jpsa/57/4/57_0190004/_html/-char/enbroiler chickenheat stressintestinal morphologyintestinal permeabilitypair-fed |
| spellingShingle | Fumika Nanto-Hara Motoi Kikusato Shyuichi Ohwada Masaaki Toyomizu Heat Stress Directly Affects Intestinal Integrity in Broiler Chickens The Journal of Poultry Science broiler chicken heat stress intestinal morphology intestinal permeability pair-fed |
| title | Heat Stress Directly Affects Intestinal Integrity in Broiler Chickens |
| title_full | Heat Stress Directly Affects Intestinal Integrity in Broiler Chickens |
| title_fullStr | Heat Stress Directly Affects Intestinal Integrity in Broiler Chickens |
| title_full_unstemmed | Heat Stress Directly Affects Intestinal Integrity in Broiler Chickens |
| title_short | Heat Stress Directly Affects Intestinal Integrity in Broiler Chickens |
| title_sort | heat stress directly affects intestinal integrity in broiler chickens |
| topic | broiler chicken heat stress intestinal morphology intestinal permeability pair-fed |
| url | https://www.jstage.jst.go.jp/article/jpsa/57/4/57_0190004/_html/-char/en |
| work_keys_str_mv | AT fumikanantohara heatstressdirectlyaffectsintestinalintegrityinbroilerchickens AT motoikikusato heatstressdirectlyaffectsintestinalintegrityinbroilerchickens AT shyuichiohwada heatstressdirectlyaffectsintestinalintegrityinbroilerchickens AT masaakitoyomizu heatstressdirectlyaffectsintestinalintegrityinbroilerchickens |