Transcriptome analysis provides new insights into plants responses under phosphate starvation in association with chilling stress
Abstract Background Chilling temperature reduces the rate of photosynthesis in plants, which is more pronounced in association with phosphate (Pi) starvation. Previous studies showed that Pi resupply improves recovery of the rate of photosynthesis in plants much better under combination of dual stre...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-01-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-021-03381-z |
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| author | Xiaoning Gao Jinsong Dong Fatemeh Rasouli Ali Kiani Pouya Ayesha T. Tahir Jun Kang |
| author_facet | Xiaoning Gao Jinsong Dong Fatemeh Rasouli Ali Kiani Pouya Ayesha T. Tahir Jun Kang |
| author_sort | Xiaoning Gao |
| collection | DOAJ |
| description | Abstract Background Chilling temperature reduces the rate of photosynthesis in plants, which is more pronounced in association with phosphate (Pi) starvation. Previous studies showed that Pi resupply improves recovery of the rate of photosynthesis in plants much better under combination of dual stresses than in non-chilled samples. However, the underlying mechanism remains poorly understood. Results In this study, RNA-seq analysis showed the expression level of 41 photosynthetic genes in plant roots increased under phosphate starvation associated with 4 °C (-P 4 °C) compared to -P 23 °C. Moreover, iron uptake increased significantly in the stem cell niche (SCN) of wild type (WT) roots in -P 4 °C. In contrast, lower iron concentrations were found in SCN of aluminum activated malate transporter 1 (almt1) and its transcription factor, sensitive to protein rhizotoxicity 1 (stop1) mutants under -P 4 °C. The Fe content examined by ICP-MS analysis in -P 4 °C treated almt1 was 98.5 ng/µg, which was only 17% of that of seedlings grown under -P 23 °C. Average plastid number in almt1 root cells under -P 4 °C was less than -P 23 °C. Furthermore, stop1 and almt1 single mutants both exhibited increased primary root elongation than WT under combined stresses. In addition, dark treatment blocked the root elongation phenotype of stop1 and almt1. Conclusions Induction of photosynthetic gene expression and increased iron accumulation in roots is required for plant adjustment to chilling in association with phosphate starvation. |
| first_indexed | 2024-12-21T00:21:28Z |
| format | Article |
| id | doaj.art-3b3a89275be741aba6c642b26f6cb608 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-21T00:21:28Z |
| publishDate | 2022-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-3b3a89275be741aba6c642b26f6cb6082022-12-21T19:22:05ZengBMCBMC Plant Biology1471-22292022-01-0122111410.1186/s12870-021-03381-zTranscriptome analysis provides new insights into plants responses under phosphate starvation in association with chilling stressXiaoning Gao0Jinsong Dong1Fatemeh Rasouli2Ali Kiani Pouya3Ayesha T. Tahir4Jun Kang5School of life sciences, Tianjin UniversityShanghai Center for Plant Stress Biology and Center of Excellence for Molecular Plant Sciences, Chinese Academy of SciencesShanghai Center for Plant Stress Biology and Center of Excellence for Molecular Plant Sciences, Chinese Academy of SciencesShanghai Center for Plant Stress Biology and Center of Excellence for Molecular Plant Sciences, Chinese Academy of SciencesDepartment of Biosciences, COMSATS University IslamabadSchool of life sciences, Tianjin UniversityAbstract Background Chilling temperature reduces the rate of photosynthesis in plants, which is more pronounced in association with phosphate (Pi) starvation. Previous studies showed that Pi resupply improves recovery of the rate of photosynthesis in plants much better under combination of dual stresses than in non-chilled samples. However, the underlying mechanism remains poorly understood. Results In this study, RNA-seq analysis showed the expression level of 41 photosynthetic genes in plant roots increased under phosphate starvation associated with 4 °C (-P 4 °C) compared to -P 23 °C. Moreover, iron uptake increased significantly in the stem cell niche (SCN) of wild type (WT) roots in -P 4 °C. In contrast, lower iron concentrations were found in SCN of aluminum activated malate transporter 1 (almt1) and its transcription factor, sensitive to protein rhizotoxicity 1 (stop1) mutants under -P 4 °C. The Fe content examined by ICP-MS analysis in -P 4 °C treated almt1 was 98.5 ng/µg, which was only 17% of that of seedlings grown under -P 23 °C. Average plastid number in almt1 root cells under -P 4 °C was less than -P 23 °C. Furthermore, stop1 and almt1 single mutants both exhibited increased primary root elongation than WT under combined stresses. In addition, dark treatment blocked the root elongation phenotype of stop1 and almt1. Conclusions Induction of photosynthetic gene expression and increased iron accumulation in roots is required for plant adjustment to chilling in association with phosphate starvation.https://doi.org/10.1186/s12870-021-03381-zPhosphate starvationChilling stressSTOP1ALMT1Fe accumulation |
| spellingShingle | Xiaoning Gao Jinsong Dong Fatemeh Rasouli Ali Kiani Pouya Ayesha T. Tahir Jun Kang Transcriptome analysis provides new insights into plants responses under phosphate starvation in association with chilling stress BMC Plant Biology Phosphate starvation Chilling stress STOP1 ALMT1 Fe accumulation |
| title | Transcriptome analysis provides new insights into plants responses under phosphate starvation in association with chilling stress |
| title_full | Transcriptome analysis provides new insights into plants responses under phosphate starvation in association with chilling stress |
| title_fullStr | Transcriptome analysis provides new insights into plants responses under phosphate starvation in association with chilling stress |
| title_full_unstemmed | Transcriptome analysis provides new insights into plants responses under phosphate starvation in association with chilling stress |
| title_short | Transcriptome analysis provides new insights into plants responses under phosphate starvation in association with chilling stress |
| title_sort | transcriptome analysis provides new insights into plants responses under phosphate starvation in association with chilling stress |
| topic | Phosphate starvation Chilling stress STOP1 ALMT1 Fe accumulation |
| url | https://doi.org/10.1186/s12870-021-03381-z |
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