Generation of inflammation-responsive astrocytes from glial progenitors derived from human pluripotent stem cells
Summary: Astrocytes are an essential component of the central nervous system for neuronal support and response to injury and disease. Here, we present a protocol to generate glial progenitor cells (GPCs) from human-induced pluripotent stem cells (iPSCs) that can be further differentiated into inflam...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-06-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722001411 |
| _version_ | 1818646517830909952 |
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| author | Renata Santos Arianna Mei Maria C. Marchetto |
| author_facet | Renata Santos Arianna Mei Maria C. Marchetto |
| author_sort | Renata Santos |
| collection | DOAJ |
| description | Summary: Astrocytes are an essential component of the central nervous system for neuronal support and response to injury and disease. Here, we present a protocol to generate glial progenitor cells (GPCs) from human-induced pluripotent stem cells (iPSCs) that can be further differentiated into inflammation-responsive astrocytes. This two-step protocol has the advantage of reducing the time of astrocyte differentiation since GPCs can be frozen and stored.For complete details on the use and execution of this protocol, please refer to Santos et al. (2017) and Vadodaria et al. (2021). |
| first_indexed | 2024-12-17T00:47:54Z |
| format | Article |
| id | doaj.art-3b3c4adc4f0946c0b6f06a31bb67dbb2 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-17T00:47:54Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-3b3c4adc4f0946c0b6f06a31bb67dbb22022-12-21T22:09:50ZengElsevierSTAR Protocols2666-16672022-06-0132101261Generation of inflammation-responsive astrocytes from glial progenitors derived from human pluripotent stem cellsRenata Santos0Arianna Mei1Maria C. Marchetto2Laboratory of Genetics, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA; Université Paris Cité, INSERM U1266, Institute of Psychiatry and Neuroscience of Paris, Laboratory of Dynamics of Neuronal Structure in Health and Disease, 102 rue de la Santé, 75014 Paris, France; Corresponding authorLaboratory of Genetics, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USALaboratory of Genetics, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA; Department of Anthropology, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA; Corresponding authorSummary: Astrocytes are an essential component of the central nervous system for neuronal support and response to injury and disease. Here, we present a protocol to generate glial progenitor cells (GPCs) from human-induced pluripotent stem cells (iPSCs) that can be further differentiated into inflammation-responsive astrocytes. This two-step protocol has the advantage of reducing the time of astrocyte differentiation since GPCs can be frozen and stored.For complete details on the use and execution of this protocol, please refer to Santos et al. (2017) and Vadodaria et al. (2021).http://www.sciencedirect.com/science/article/pii/S2666166722001411Cell cultureNeuroscienceStem CellsCell Differentiation |
| spellingShingle | Renata Santos Arianna Mei Maria C. Marchetto Generation of inflammation-responsive astrocytes from glial progenitors derived from human pluripotent stem cells STAR Protocols Cell culture Neuroscience Stem Cells Cell Differentiation |
| title | Generation of inflammation-responsive astrocytes from glial progenitors derived from human pluripotent stem cells |
| title_full | Generation of inflammation-responsive astrocytes from glial progenitors derived from human pluripotent stem cells |
| title_fullStr | Generation of inflammation-responsive astrocytes from glial progenitors derived from human pluripotent stem cells |
| title_full_unstemmed | Generation of inflammation-responsive astrocytes from glial progenitors derived from human pluripotent stem cells |
| title_short | Generation of inflammation-responsive astrocytes from glial progenitors derived from human pluripotent stem cells |
| title_sort | generation of inflammation responsive astrocytes from glial progenitors derived from human pluripotent stem cells |
| topic | Cell culture Neuroscience Stem Cells Cell Differentiation |
| url | http://www.sciencedirect.com/science/article/pii/S2666166722001411 |
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