Biodistribution of Quantum Dots-Labelled Halloysite Nanotubes: A <i>Caenorhabditis elegans</i> In Vivo Study
Halloysite is a promising building block in nanoarchitectonics of functional materials, especially in the development of novel biomaterials and smart coatings. Understanding the behavior of materials produced using halloysite nanotubes within living organisms is essential for their safe applications...
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2021-09-01
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| Online Access: | https://www.mdpi.com/1996-1944/14/19/5469 |
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| author | Anna Stavitskaya Gölnur Fakhrullina Läysän Nigamatzyanova Eliza Sitmukhanova Elnara Khusnetdenova Rawil Fakhrullin Vladimir Vinokurov |
| author_facet | Anna Stavitskaya Gölnur Fakhrullina Läysän Nigamatzyanova Eliza Sitmukhanova Elnara Khusnetdenova Rawil Fakhrullin Vladimir Vinokurov |
| author_sort | Anna Stavitskaya |
| collection | DOAJ |
| description | Halloysite is a promising building block in nanoarchitectonics of functional materials, especially in the development of novel biomaterials and smart coatings. Understanding the behavior of materials produced using halloysite nanotubes within living organisms is essential for their safe applications. In this study, quantum dots of different compositions were synthesized on the surface of modified clay nanotubes, and the biodistribution of this hybrid material was monitored within <i>Caenorhabditis elegans</i> nematodes. The influence of the modification agent as well as the particles’ composition on physicochemical properties of hybrid nanomaterials was investigated. Several microscopy techniques, such as fluorescence and dark-field microscopy, were compared in monitoring the distribution of nanomaterials in nematodes’ organisms. The effects of QDs-halloysite composites on the nematodes’ life cycle were investigated in vivo. Our fluorescent hybrid probes induced no acute toxic effects in model organisms. The stable fluorescence and low toxicity towards the organisms suggest that the proposed synthesis procedure yields safe nanoarchitectonic materials that will be helpful in monitoring the behavior of nanomaterials inside living cells and organisms. |
| first_indexed | 2024-03-10T06:56:47Z |
| format | Article |
| id | doaj.art-3b3e026b2af640ca8203290e6603ff1d |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-10T06:56:47Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-3b3e026b2af640ca8203290e6603ff1d2023-11-22T16:22:20ZengMDPI AGMaterials1996-19442021-09-011419546910.3390/ma14195469Biodistribution of Quantum Dots-Labelled Halloysite Nanotubes: A <i>Caenorhabditis elegans</i> In Vivo StudyAnna Stavitskaya0Gölnur Fakhrullina1Läysän Nigamatzyanova2Eliza Sitmukhanova3Elnara Khusnetdenova4Rawil Fakhrullin5Vladimir Vinokurov6Department of Physical and Colloid Chemistry, Russian State University of Oil and Gas (National Research University), Leninsky Prospect, 65, 119991 Moscow, RussiaInstitute of Fundamental Medicine and Biology, Kazan Federal University, Kreml urami 18, 420008 Kazan, Republic of Tatarstan, RussiaInstitute of Fundamental Medicine and Biology, Kazan Federal University, Kreml urami 18, 420008 Kazan, Republic of Tatarstan, RussiaDepartment of Physical and Colloid Chemistry, Russian State University of Oil and Gas (National Research University), Leninsky Prospect, 65, 119991 Moscow, RussiaDepartment of Physical and Colloid Chemistry, Russian State University of Oil and Gas (National Research University), Leninsky Prospect, 65, 119991 Moscow, RussiaDepartment of Physical and Colloid Chemistry, Russian State University of Oil and Gas (National Research University), Leninsky Prospect, 65, 119991 Moscow, RussiaDepartment of Physical and Colloid Chemistry, Russian State University of Oil and Gas (National Research University), Leninsky Prospect, 65, 119991 Moscow, RussiaHalloysite is a promising building block in nanoarchitectonics of functional materials, especially in the development of novel biomaterials and smart coatings. Understanding the behavior of materials produced using halloysite nanotubes within living organisms is essential for their safe applications. In this study, quantum dots of different compositions were synthesized on the surface of modified clay nanotubes, and the biodistribution of this hybrid material was monitored within <i>Caenorhabditis elegans</i> nematodes. The influence of the modification agent as well as the particles’ composition on physicochemical properties of hybrid nanomaterials was investigated. Several microscopy techniques, such as fluorescence and dark-field microscopy, were compared in monitoring the distribution of nanomaterials in nematodes’ organisms. The effects of QDs-halloysite composites on the nematodes’ life cycle were investigated in vivo. Our fluorescent hybrid probes induced no acute toxic effects in model organisms. The stable fluorescence and low toxicity towards the organisms suggest that the proposed synthesis procedure yields safe nanoarchitectonic materials that will be helpful in monitoring the behavior of nanomaterials inside living cells and organisms.https://www.mdpi.com/1996-1944/14/19/5469<i>Caenorhabditis elegans</i>quantum dotsin vivo imagingreproductive toxicityhalloysite |
| spellingShingle | Anna Stavitskaya Gölnur Fakhrullina Läysän Nigamatzyanova Eliza Sitmukhanova Elnara Khusnetdenova Rawil Fakhrullin Vladimir Vinokurov Biodistribution of Quantum Dots-Labelled Halloysite Nanotubes: A <i>Caenorhabditis elegans</i> In Vivo Study Materials <i>Caenorhabditis elegans</i> quantum dots in vivo imaging reproductive toxicity halloysite |
| title | Biodistribution of Quantum Dots-Labelled Halloysite Nanotubes: A <i>Caenorhabditis elegans</i> In Vivo Study |
| title_full | Biodistribution of Quantum Dots-Labelled Halloysite Nanotubes: A <i>Caenorhabditis elegans</i> In Vivo Study |
| title_fullStr | Biodistribution of Quantum Dots-Labelled Halloysite Nanotubes: A <i>Caenorhabditis elegans</i> In Vivo Study |
| title_full_unstemmed | Biodistribution of Quantum Dots-Labelled Halloysite Nanotubes: A <i>Caenorhabditis elegans</i> In Vivo Study |
| title_short | Biodistribution of Quantum Dots-Labelled Halloysite Nanotubes: A <i>Caenorhabditis elegans</i> In Vivo Study |
| title_sort | biodistribution of quantum dots labelled halloysite nanotubes a i caenorhabditis elegans i in vivo study |
| topic | <i>Caenorhabditis elegans</i> quantum dots in vivo imaging reproductive toxicity halloysite |
| url | https://www.mdpi.com/1996-1944/14/19/5469 |
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