Sphingolipids in Childhood Asthma and Obesity (SOAP Study): A Protocol of a Cross-Sectional Study
Asthma and obesity are two of the most common chronic conditions in children and adolescents. There is increasing evidence that sphingolipid metabolism is altered in childhood asthma and is linked to airway hyperreactivity. Dysregulated sphingolipid metabolism is also reported in obesity. However, t...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-11-01
|
| Series: | Metabolites |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-1989/13/11/1146 |
| _version_ | 1797458430009540608 |
|---|---|
| author | Belavendra Antonisamy Harshita Shailesh Yahya Hani Lina Hayati M. Ahmed Safa Noor Salma Yahya Ahmed Mohamed Alfaki Abidan Muhayimana Shana Sunny Jacob Saroja Kotegar Balayya Oleksandr Soloviov Li Liu Lisa Sara Mathew Kun Wang Sara Tomei Alia Al Massih Rebecca Mathew Mohammed Yousuf Karim Manjunath Ramanjaneya Stefan Worgall Ibrahim A. Janahi |
| author_facet | Belavendra Antonisamy Harshita Shailesh Yahya Hani Lina Hayati M. Ahmed Safa Noor Salma Yahya Ahmed Mohamed Alfaki Abidan Muhayimana Shana Sunny Jacob Saroja Kotegar Balayya Oleksandr Soloviov Li Liu Lisa Sara Mathew Kun Wang Sara Tomei Alia Al Massih Rebecca Mathew Mohammed Yousuf Karim Manjunath Ramanjaneya Stefan Worgall Ibrahim A. Janahi |
| author_sort | Belavendra Antonisamy |
| collection | DOAJ |
| description | Asthma and obesity are two of the most common chronic conditions in children and adolescents. There is increasing evidence that sphingolipid metabolism is altered in childhood asthma and is linked to airway hyperreactivity. Dysregulated sphingolipid metabolism is also reported in obesity. However, the functional link between sphingolipid metabolism, asthma, and obesity is not completely understood. This paper describes the protocol of an ongoing study on sphingolipids that aims to examine the pathophysiology of sphingolipids in childhood asthma and obesity. In addition, this study aims to explore the novel biomarkers through a comprehensive multi-omics approach including genomics, genome-wide DNA methylation, RNA-Seq, microRNA (miRNA) profiling, lipidomics, metabolomics, and cytokine profiling. This is a cross-sectional study aiming to recruit 440 children from different groups: children with asthma and normal weight (<i>n</i> = 100), asthma with overweight or obesity (<i>n</i> = 100), overweight or obesity (<i>n</i> = 100), normal weight (<i>n</i> = 70), and siblings of asthmatic children with normal weight, overweight, or obesity (<i>n</i> = 70). These participants will be recruited from the pediatric pulmonology, pediatric endocrinology, and general pediatric outpatient clinics at Sidra Medicine, Doha, Qatar. Information will be obtained from self-reported questionnaires on asthma, quality of life, food frequency (FFQ), and a 3-day food diary that are completed by the children and their parents. Clinical measurements will include anthropometry, blood pressure, biochemistry, bioelectrical impedance, and pulmonary function tests. Blood samples will be obtained for sphingolipid analysis, serine palmitoyltransferase (SPT) assay, whole-genome sequencing (WGS), genome-wide DNA methylation study, RNA-Seq, miRNA profiling, metabolomics, lipidomics, and cytokine analysis. Group comparisons of continuous outcome variables will be carried out by a one-way analysis of variance or the Kruskal–Wallis test using an appropriate pairwise multiple comparison test. The chi-squared test or a Fisher’s exact test will be used to test the associations between categorical variables. Finally, multivariate analysis will be carried out to integrate the clinical data with multi-omics data. This study will help us to understand the role of dysregulated sphingolipid metabolism in obesity and asthma. In addition, the multi-omics data from the study will help to identify novel genetic and epigenetic signatures, inflammatory markers, and mechanistic pathways that link asthma and obesity in children. Furthermore, the integration of clinical and multi-omics data will help us to uncover the potential interactions between these diseases and to offer a new paradigm for the treatment of pediatric obesity-associated asthma. |
| first_indexed | 2024-03-09T16:37:00Z |
| format | Article |
| id | doaj.art-3b3f55e70637419aa7c7ccebe3691593 |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-09T16:37:00Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-3b3f55e70637419aa7c7ccebe36915932023-11-24T14:55:29ZengMDPI AGMetabolites2218-19892023-11-011311114610.3390/metabo13111146Sphingolipids in Childhood Asthma and Obesity (SOAP Study): A Protocol of a Cross-Sectional StudyBelavendra Antonisamy0Harshita Shailesh1Yahya Hani2Lina Hayati M. Ahmed3Safa Noor4Salma Yahya Ahmed5Mohamed Alfaki6Abidan Muhayimana7Shana Sunny Jacob8Saroja Kotegar Balayya9Oleksandr Soloviov10Li Liu11Lisa Sara Mathew12Kun Wang13Sara Tomei14Alia Al Massih15Rebecca Mathew16Mohammed Yousuf Karim17Manjunath Ramanjaneya18Stefan Worgall19Ibrahim A. Janahi20Department of Pediatric Medicine, Sidra Medicine, Doha P.O. Box 26999, QatarDepartment of Pediatric Medicine, Sidra Medicine, Doha P.O. Box 26999, QatarDepartment of Pediatric Medicine, Sidra Medicine, Doha P.O. Box 26999, QatarDepartment of Pediatric Medicine, Sidra Medicine, Doha P.O. Box 26999, QatarDepartment of Pediatric Medicine, Sidra Medicine, Doha P.O. Box 26999, QatarDepartment of Pediatric Medicine, Sidra Medicine, Doha P.O. Box 26999, QatarDepartment of Pediatric Medicine, Sidra Medicine, Doha P.O. Box 26999, QatarDepartment of Pediatric Medicine, Sidra Medicine, Doha P.O. Box 26999, QatarAnalytical Chemistry Core, Advanced Diagnostic Core Facilities, Sidra Medicine, Doha P.O. Box 26999, QatarAnalytical Chemistry Core, Advanced Diagnostic Core Facilities, Sidra Medicine, Doha P.O. Box 26999, QatarClinical Genomics Laboratory, Integrated Genomics Services, Sidra Medicine, Doha P.O. Box 26999, QatarClinical Genomics Laboratory, Integrated Genomics Services, Sidra Medicine, Doha P.O. Box 26999, QatarClinical Genomics Laboratory, Integrated Genomics Services, Sidra Medicine, Doha P.O. Box 26999, QatarClinical Genomics Laboratory, Integrated Genomics Services, Sidra Medicine, Doha P.O. Box 26999, QatarOmics Core, Integrated Genomics Services, Sidra Medicine, Doha P.O. Box 26999, QatarOmics Core, Integrated Genomics Services, Sidra Medicine, Doha P.O. Box 26999, QatarOmics Core, Integrated Genomics Services, Sidra Medicine, Doha P.O. Box 26999, QatarDepartment of Pathology, Sidra Medicine, Doha P.O. Box 26999, QatarQatar Metabolic Institute, Hamad Medical Corporation, Doha P.O. Box 3050, QatarDepartment of Pediatrics, Weill Cornell Medicine, New York, NY 10021, USADepartment of Pediatric Medicine, Sidra Medicine, Doha P.O. Box 26999, QatarAsthma and obesity are two of the most common chronic conditions in children and adolescents. There is increasing evidence that sphingolipid metabolism is altered in childhood asthma and is linked to airway hyperreactivity. Dysregulated sphingolipid metabolism is also reported in obesity. However, the functional link between sphingolipid metabolism, asthma, and obesity is not completely understood. This paper describes the protocol of an ongoing study on sphingolipids that aims to examine the pathophysiology of sphingolipids in childhood asthma and obesity. In addition, this study aims to explore the novel biomarkers through a comprehensive multi-omics approach including genomics, genome-wide DNA methylation, RNA-Seq, microRNA (miRNA) profiling, lipidomics, metabolomics, and cytokine profiling. This is a cross-sectional study aiming to recruit 440 children from different groups: children with asthma and normal weight (<i>n</i> = 100), asthma with overweight or obesity (<i>n</i> = 100), overweight or obesity (<i>n</i> = 100), normal weight (<i>n</i> = 70), and siblings of asthmatic children with normal weight, overweight, or obesity (<i>n</i> = 70). These participants will be recruited from the pediatric pulmonology, pediatric endocrinology, and general pediatric outpatient clinics at Sidra Medicine, Doha, Qatar. Information will be obtained from self-reported questionnaires on asthma, quality of life, food frequency (FFQ), and a 3-day food diary that are completed by the children and their parents. Clinical measurements will include anthropometry, blood pressure, biochemistry, bioelectrical impedance, and pulmonary function tests. Blood samples will be obtained for sphingolipid analysis, serine palmitoyltransferase (SPT) assay, whole-genome sequencing (WGS), genome-wide DNA methylation study, RNA-Seq, miRNA profiling, metabolomics, lipidomics, and cytokine analysis. Group comparisons of continuous outcome variables will be carried out by a one-way analysis of variance or the Kruskal–Wallis test using an appropriate pairwise multiple comparison test. The chi-squared test or a Fisher’s exact test will be used to test the associations between categorical variables. Finally, multivariate analysis will be carried out to integrate the clinical data with multi-omics data. This study will help us to understand the role of dysregulated sphingolipid metabolism in obesity and asthma. In addition, the multi-omics data from the study will help to identify novel genetic and epigenetic signatures, inflammatory markers, and mechanistic pathways that link asthma and obesity in children. Furthermore, the integration of clinical and multi-omics data will help us to uncover the potential interactions between these diseases and to offer a new paradigm for the treatment of pediatric obesity-associated asthma.https://www.mdpi.com/2218-1989/13/11/1146childrenasthmaobesitysphingolipidsserine palmitoyltransferase |
| spellingShingle | Belavendra Antonisamy Harshita Shailesh Yahya Hani Lina Hayati M. Ahmed Safa Noor Salma Yahya Ahmed Mohamed Alfaki Abidan Muhayimana Shana Sunny Jacob Saroja Kotegar Balayya Oleksandr Soloviov Li Liu Lisa Sara Mathew Kun Wang Sara Tomei Alia Al Massih Rebecca Mathew Mohammed Yousuf Karim Manjunath Ramanjaneya Stefan Worgall Ibrahim A. Janahi Sphingolipids in Childhood Asthma and Obesity (SOAP Study): A Protocol of a Cross-Sectional Study Metabolites children asthma obesity sphingolipids serine palmitoyltransferase |
| title | Sphingolipids in Childhood Asthma and Obesity (SOAP Study): A Protocol of a Cross-Sectional Study |
| title_full | Sphingolipids in Childhood Asthma and Obesity (SOAP Study): A Protocol of a Cross-Sectional Study |
| title_fullStr | Sphingolipids in Childhood Asthma and Obesity (SOAP Study): A Protocol of a Cross-Sectional Study |
| title_full_unstemmed | Sphingolipids in Childhood Asthma and Obesity (SOAP Study): A Protocol of a Cross-Sectional Study |
| title_short | Sphingolipids in Childhood Asthma and Obesity (SOAP Study): A Protocol of a Cross-Sectional Study |
| title_sort | sphingolipids in childhood asthma and obesity soap study a protocol of a cross sectional study |
| topic | children asthma obesity sphingolipids serine palmitoyltransferase |
| url | https://www.mdpi.com/2218-1989/13/11/1146 |
| work_keys_str_mv | AT belavendraantonisamy sphingolipidsinchildhoodasthmaandobesitysoapstudyaprotocolofacrosssectionalstudy AT harshitashailesh sphingolipidsinchildhoodasthmaandobesitysoapstudyaprotocolofacrosssectionalstudy AT yahyahani sphingolipidsinchildhoodasthmaandobesitysoapstudyaprotocolofacrosssectionalstudy AT linahayatimahmed sphingolipidsinchildhoodasthmaandobesitysoapstudyaprotocolofacrosssectionalstudy AT safanoor sphingolipidsinchildhoodasthmaandobesitysoapstudyaprotocolofacrosssectionalstudy AT salmayahyaahmed sphingolipidsinchildhoodasthmaandobesitysoapstudyaprotocolofacrosssectionalstudy AT mohamedalfaki sphingolipidsinchildhoodasthmaandobesitysoapstudyaprotocolofacrosssectionalstudy AT abidanmuhayimana sphingolipidsinchildhoodasthmaandobesitysoapstudyaprotocolofacrosssectionalstudy AT shanasunnyjacob sphingolipidsinchildhoodasthmaandobesitysoapstudyaprotocolofacrosssectionalstudy AT sarojakotegarbalayya sphingolipidsinchildhoodasthmaandobesitysoapstudyaprotocolofacrosssectionalstudy AT oleksandrsoloviov sphingolipidsinchildhoodasthmaandobesitysoapstudyaprotocolofacrosssectionalstudy AT liliu sphingolipidsinchildhoodasthmaandobesitysoapstudyaprotocolofacrosssectionalstudy AT lisasaramathew sphingolipidsinchildhoodasthmaandobesitysoapstudyaprotocolofacrosssectionalstudy AT kunwang sphingolipidsinchildhoodasthmaandobesitysoapstudyaprotocolofacrosssectionalstudy AT saratomei sphingolipidsinchildhoodasthmaandobesitysoapstudyaprotocolofacrosssectionalstudy AT aliaalmassih sphingolipidsinchildhoodasthmaandobesitysoapstudyaprotocolofacrosssectionalstudy AT rebeccamathew sphingolipidsinchildhoodasthmaandobesitysoapstudyaprotocolofacrosssectionalstudy AT mohammedyousufkarim sphingolipidsinchildhoodasthmaandobesitysoapstudyaprotocolofacrosssectionalstudy AT manjunathramanjaneya sphingolipidsinchildhoodasthmaandobesitysoapstudyaprotocolofacrosssectionalstudy AT stefanworgall sphingolipidsinchildhoodasthmaandobesitysoapstudyaprotocolofacrosssectionalstudy AT ibrahimajanahi sphingolipidsinchildhoodasthmaandobesitysoapstudyaprotocolofacrosssectionalstudy |