Phytotoxic Action of Silver Nanoparticles on <i>Lemna minor</i>: Multi-Parameter Analysis of Different Physiological Processes
Considering the widespread use of silver nanoparticles (AgNPs) and their consequent build-up in waterways, there is a concern about the hazardous effect of AgNPs for aquatic ecosystems. The aim of this study was to clarify the mechanism of the action of AgNPs on duckweed (<i>Lemna minor</i&...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/2/343 |
| _version_ | 1797437632168329216 |
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| author | Katarina Glavaš Ljubimir Ana-Marija Domijan Sandra Radić Brkanac |
| author_facet | Katarina Glavaš Ljubimir Ana-Marija Domijan Sandra Radić Brkanac |
| author_sort | Katarina Glavaš Ljubimir |
| collection | DOAJ |
| description | Considering the widespread use of silver nanoparticles (AgNPs) and their consequent build-up in waterways, there is a concern about the hazardous effect of AgNPs for aquatic ecosystems. The aim of this study was to clarify the mechanism of the action of AgNPs on duckweed (<i>Lemna minor</i> L.) by evaluating multiple parameters in different physiological processes. Duckweed was treated with AgNPs in a concentration range of 0.5 to 5 mg/L over a 7-day period. The analysis revealed that the AgNP-treated duckweed accumulated Ag in accordance with increasing AgNP concentrations. Furthermore, higher concentrations (2 and 5 mg/L) of AgNPs negatively affected N, P and especially K and Mg levels in the plant tissue. Accordingly, the plant growth and photosynthetic parameters were more inhibited in response to higher concentrations of AgNPs. Nanosilver significantly increased the generation of ROS at higher concentrations, although lipid peroxidation was significant even at the lowest concentration of AgNPs. However, defense mechanisms were able to counteract AgNP-induced oxidative stress and balance the intracellular redox status, as evidenced by increased activities of the main detoxification enzymes. With this experimental setting, AgNPs exhibited a relatively weak phytotoxicity at 0.5 and 1 mg/L; nevertheless, silver in a nano form poses a hazard for plants, considering its continuous release into aquatic environments. |
| first_indexed | 2024-03-09T11:25:08Z |
| format | Article |
| id | doaj.art-3b3f8161537d42b69229527fef746d81 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T11:25:08Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-3b3f8161537d42b69229527fef746d812023-12-01T00:05:16ZengMDPI AGPlants2223-77472023-01-0112234310.3390/plants12020343Phytotoxic Action of Silver Nanoparticles on <i>Lemna minor</i>: Multi-Parameter Analysis of Different Physiological ProcessesKatarina Glavaš Ljubimir0Ana-Marija Domijan1Sandra Radić Brkanac2Croatian Waters, Main Water Management Laboratory, 21 000 Split, CroatiaDepartment of Pharmaceutical Botany, Faculty of Pharmacy and Biochemistry, University of Zagreb, 10 000 Zagreb, CroatiaDepartment of Biology, Faculty of Science, University of Zagreb, 10 000 Zagreb, CroatiaConsidering the widespread use of silver nanoparticles (AgNPs) and their consequent build-up in waterways, there is a concern about the hazardous effect of AgNPs for aquatic ecosystems. The aim of this study was to clarify the mechanism of the action of AgNPs on duckweed (<i>Lemna minor</i> L.) by evaluating multiple parameters in different physiological processes. Duckweed was treated with AgNPs in a concentration range of 0.5 to 5 mg/L over a 7-day period. The analysis revealed that the AgNP-treated duckweed accumulated Ag in accordance with increasing AgNP concentrations. Furthermore, higher concentrations (2 and 5 mg/L) of AgNPs negatively affected N, P and especially K and Mg levels in the plant tissue. Accordingly, the plant growth and photosynthetic parameters were more inhibited in response to higher concentrations of AgNPs. Nanosilver significantly increased the generation of ROS at higher concentrations, although lipid peroxidation was significant even at the lowest concentration of AgNPs. However, defense mechanisms were able to counteract AgNP-induced oxidative stress and balance the intracellular redox status, as evidenced by increased activities of the main detoxification enzymes. With this experimental setting, AgNPs exhibited a relatively weak phytotoxicity at 0.5 and 1 mg/L; nevertheless, silver in a nano form poses a hazard for plants, considering its continuous release into aquatic environments.https://www.mdpi.com/2223-7747/12/2/343antioxidative enzymeglutathionemalondialdehydenanosilverphotosystem II |
| spellingShingle | Katarina Glavaš Ljubimir Ana-Marija Domijan Sandra Radić Brkanac Phytotoxic Action of Silver Nanoparticles on <i>Lemna minor</i>: Multi-Parameter Analysis of Different Physiological Processes Plants antioxidative enzyme glutathione malondialdehyde nanosilver photosystem II |
| title | Phytotoxic Action of Silver Nanoparticles on <i>Lemna minor</i>: Multi-Parameter Analysis of Different Physiological Processes |
| title_full | Phytotoxic Action of Silver Nanoparticles on <i>Lemna minor</i>: Multi-Parameter Analysis of Different Physiological Processes |
| title_fullStr | Phytotoxic Action of Silver Nanoparticles on <i>Lemna minor</i>: Multi-Parameter Analysis of Different Physiological Processes |
| title_full_unstemmed | Phytotoxic Action of Silver Nanoparticles on <i>Lemna minor</i>: Multi-Parameter Analysis of Different Physiological Processes |
| title_short | Phytotoxic Action of Silver Nanoparticles on <i>Lemna minor</i>: Multi-Parameter Analysis of Different Physiological Processes |
| title_sort | phytotoxic action of silver nanoparticles on i lemna minor i multi parameter analysis of different physiological processes |
| topic | antioxidative enzyme glutathione malondialdehyde nanosilver photosystem II |
| url | https://www.mdpi.com/2223-7747/12/2/343 |
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