Lactic Acid Bacteria-Fermented Diet Containing Bacterial Extracellular Vesicles Inhibited Pathogenic Bacteria in Striped Beakfish (<i>Oplegnathus fasciatus</i>)
In recent years, probiotics have received considerable attention for improving the health of aquaculture organisms, such as fish and shrimp, by stimulating immune activity and increasing growth rates. <i>Oplegnathus fasciatus</i> is a common and economically important cultured fish speci...
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| Format: | Article |
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MDPI AG
2024-01-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/10/1/49 |
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| author | Bao-Hong Lee Yeh-Fang Hu Yu-Ting Chu Yu-Sheng Wu Wei-Hsuan Hsu Fan-Hua Nan |
| author_facet | Bao-Hong Lee Yeh-Fang Hu Yu-Ting Chu Yu-Sheng Wu Wei-Hsuan Hsu Fan-Hua Nan |
| author_sort | Bao-Hong Lee |
| collection | DOAJ |
| description | In recent years, probiotics have received considerable attention for improving the health of aquaculture organisms, such as fish and shrimp, by stimulating immune activity and increasing growth rates. <i>Oplegnathus fasciatus</i> is a common and economically important cultured fish species in Asia. In this study, we aimed to investigate the potential of lactic acid bacteria (LAB; <i>Limosilactobacillus reuteri</i>)-fermented feed to promote growth and enhance immune function in <i>O. fasciatus</i>. The feed contained the highest proportion of LAB after <i>L. reuteri</i> fermentation for 3 days in anaerobic conditions. <i>Oplegnathus fasciatus</i> was fed LAB-fermented feed for 30 days. The administration of LAB-fermented feed (live bacteria > 10<sup>9</sup> CFU/g) significantly increased the growth rate (weight gain = 174.8%; FCR = 4.23) and intestinal probiotic levels of <i>O. fasciatus</i>. After LAB-fermented feeding, the immunity index was evaluated by superoxide anion production, the phagocytic activity of leukocytes, and bactericidal and lysozyme activities in the serum of <i>O. fasciatus.</i> We found that LAB-fermented feed treatment potentially elevated the proportions of intestinal <i>Bifidobacterium</i>, <i>Blautia</i>, and <i>Dorea</i> species and reduced pathogenic bacterial growth (<i>Acinetobacter</i>, <i>Escherichia_Shigella,</i> and <i>Megasphaera</i>) in <i>O. fasciatus</i>. This study demonstrated that LAB-fermented feed containing extracellular vesicles improves growth performance and the inhibition of pathogenic <i>Acinetobacter baumannii</i>. |
| first_indexed | 2024-03-08T10:57:05Z |
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| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-08T10:57:05Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-3b40b19af1be47a29df683899cfc83bf2024-01-26T16:23:51ZengMDPI AGFermentation2311-56372024-01-011014910.3390/fermentation10010049Lactic Acid Bacteria-Fermented Diet Containing Bacterial Extracellular Vesicles Inhibited Pathogenic Bacteria in Striped Beakfish (<i>Oplegnathus fasciatus</i>)Bao-Hong Lee0Yeh-Fang Hu1Yu-Ting Chu2Yu-Sheng Wu3Wei-Hsuan Hsu4Fan-Hua Nan5Department of Aquaculture, National Taiwan Ocean University, Keelung 202301, TaiwanDepartment of Aquaculture, National Taiwan Ocean University, Keelung 202301, TaiwanDepartment of Aquaculture, National Taiwan Ocean University, Keelung 202301, TaiwanDepartment of Aquaculture, National Pingtung University of Science and Technology, Pingtung 912301, TaiwanDepartment of Food Safety/Hygiene and Risk Management, National Cheng Kung University, Tainan 701401, TaiwanDepartment of Aquaculture, National Taiwan Ocean University, Keelung 202301, TaiwanIn recent years, probiotics have received considerable attention for improving the health of aquaculture organisms, such as fish and shrimp, by stimulating immune activity and increasing growth rates. <i>Oplegnathus fasciatus</i> is a common and economically important cultured fish species in Asia. In this study, we aimed to investigate the potential of lactic acid bacteria (LAB; <i>Limosilactobacillus reuteri</i>)-fermented feed to promote growth and enhance immune function in <i>O. fasciatus</i>. The feed contained the highest proportion of LAB after <i>L. reuteri</i> fermentation for 3 days in anaerobic conditions. <i>Oplegnathus fasciatus</i> was fed LAB-fermented feed for 30 days. The administration of LAB-fermented feed (live bacteria > 10<sup>9</sup> CFU/g) significantly increased the growth rate (weight gain = 174.8%; FCR = 4.23) and intestinal probiotic levels of <i>O. fasciatus</i>. After LAB-fermented feeding, the immunity index was evaluated by superoxide anion production, the phagocytic activity of leukocytes, and bactericidal and lysozyme activities in the serum of <i>O. fasciatus.</i> We found that LAB-fermented feed treatment potentially elevated the proportions of intestinal <i>Bifidobacterium</i>, <i>Blautia</i>, and <i>Dorea</i> species and reduced pathogenic bacterial growth (<i>Acinetobacter</i>, <i>Escherichia_Shigella,</i> and <i>Megasphaera</i>) in <i>O. fasciatus</i>. This study demonstrated that LAB-fermented feed containing extracellular vesicles improves growth performance and the inhibition of pathogenic <i>Acinetobacter baumannii</i>.https://www.mdpi.com/2311-5637/10/1/49probiotics<i>Oplegnathus fasciatus</i><i>Limosilactobacillus reuteri</i>pathogenic bacteriamicrobiota |
| spellingShingle | Bao-Hong Lee Yeh-Fang Hu Yu-Ting Chu Yu-Sheng Wu Wei-Hsuan Hsu Fan-Hua Nan Lactic Acid Bacteria-Fermented Diet Containing Bacterial Extracellular Vesicles Inhibited Pathogenic Bacteria in Striped Beakfish (<i>Oplegnathus fasciatus</i>) Fermentation probiotics <i>Oplegnathus fasciatus</i> <i>Limosilactobacillus reuteri</i> pathogenic bacteria microbiota |
| title | Lactic Acid Bacteria-Fermented Diet Containing Bacterial Extracellular Vesicles Inhibited Pathogenic Bacteria in Striped Beakfish (<i>Oplegnathus fasciatus</i>) |
| title_full | Lactic Acid Bacteria-Fermented Diet Containing Bacterial Extracellular Vesicles Inhibited Pathogenic Bacteria in Striped Beakfish (<i>Oplegnathus fasciatus</i>) |
| title_fullStr | Lactic Acid Bacteria-Fermented Diet Containing Bacterial Extracellular Vesicles Inhibited Pathogenic Bacteria in Striped Beakfish (<i>Oplegnathus fasciatus</i>) |
| title_full_unstemmed | Lactic Acid Bacteria-Fermented Diet Containing Bacterial Extracellular Vesicles Inhibited Pathogenic Bacteria in Striped Beakfish (<i>Oplegnathus fasciatus</i>) |
| title_short | Lactic Acid Bacteria-Fermented Diet Containing Bacterial Extracellular Vesicles Inhibited Pathogenic Bacteria in Striped Beakfish (<i>Oplegnathus fasciatus</i>) |
| title_sort | lactic acid bacteria fermented diet containing bacterial extracellular vesicles inhibited pathogenic bacteria in striped beakfish i oplegnathus fasciatus i |
| topic | probiotics <i>Oplegnathus fasciatus</i> <i>Limosilactobacillus reuteri</i> pathogenic bacteria microbiota |
| url | https://www.mdpi.com/2311-5637/10/1/49 |
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